Improved detection of botulinum type e by rational design of a new peptide substrate for endopeptidase-mass spectrometry assay

Analytical Biochemistry

Volumn 456, Issue 1, 2014, Pages 50-52

  Rosen, Osnat ^a   Feldberg, Liron ^a   Gura, Sigalit ^a   Zichel, Ran ^a  

^a ISRAEL INSTITUTE FOR BIOLOGICAL RESEARCH   (Israel)

Author keywords

Botulinum neurotoxin; Detection; Mass spectrometry; Peptide substrate; UPLC

Indexed keywords

ERROR DETECTION; MASS SPECTROMETRY;

BOTULINUM NEUROTOXINS; CLEAVAGE PRODUCTS; PEPTIDE SUBSTRATES; RATIONAL DESIGN; STRUCTURAL INTERACTIONS; SUBSTRATE PEPTIDES; TOXIC SUBSTANCES; UPLC;

PEPTIDES;

ARGININE; BOTULINUM TOXIN E; ISOLEUCINE; PROTEINASE; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; BOTULINUM TOXIN; PEPTIDE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANALYTIC METHOD; ARTICLE; CARBOXY TERMINAL SEQUENCE; HUMAN; IN VITRO STUDY; MASS SPECTROMETRY; MOLECULAR INTERACTION; MOLECULAR RECOGNITION; MULTIPLE REACTION MONITORING; NONHUMAN; PRIORITY JOURNAL; PROTEIN CLEAVAGE; QUADRUPOLE MASS SPECTROMETRY; SENSITIVITY ANALYSIS; TANDEM MASS SPECTROMETRY; TIME OF FLIGHT MASS SPECTROMETRY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; ULTRAVIOLET SPECTROSCOPY; CHEMISTRY; DRUG DESIGN; IMMUNOASSAY; METABOLISM; MOLECULAR GENETICS; PROCEDURES;

AMINO ACID SEQUENCE; BOTULINUM TOXINS; DRUG DESIGN; IMMUNOASSAY; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDES;

EID: 84900440992     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.03.024     Document Type: Article

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