A general method for site specific fluorescent labeling of recombinant chemokines

PLoS ONE

Volumn 9, Issue 1, 2014, Pages

  Kawamura, Tetsuya ^a   Stephens, Bryan ^a,b   Qin, Ling ^a   Yin, Xin ^c,d,e   Dores, Michael R ^f   Smith, Thomas H ^b,f   Grimsey, Neil ^f   Abagyan, Ruben ^a   Trejo, JoAnn ^f   Kufareva, Irina ^a   Fuster, Mark M ^d,e   Salanga, Catherina L ^a   Handel, Tracy M ^a  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c HUAIHAI INSTITUTE OF TECHNOLOGY   (China)

^d VA SAN DIEGO HEALTHCARE SYSTEM   (United States)

^e UNIVERSITY OF CALIFORNIA   (United States)

^f San Diego   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CHEMOKINE; CHEMOKINE RECEPTOR; CHEMOKINE RECEPTOR CCR2; CHEMOKINE RECEPTOR CXCR4; COENZYME A; FLUORESCENT DYE; GLYCOSAMINOGLYCAN; MONOCYTE CHEMOTACTIC PROTEIN 1; PHOSPHOPANTETHEINYL TRANSFERASE; RECOMBINANT CHEMOKINE; RECOMBINANT PROTEIN; SECONDARY LYMPHOID TISSUE CHEMOKINE; STROMAL CELL DERIVED FACTOR 1; TRANSFERASE; UNCLASSIFIED DRUG; CCR2 PROTEIN, HUMAN; CCR7 PROTEIN, HUMAN; CHEMOKINE RECEPTOR CCR7; CXCR4 PROTEIN, HUMAN;

ARTICLE; CALCIUM MOBILIZATION ASSAY; CELL MIGRATION ASSAY; CELLULAR DISTRIBUTION; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE ANALYSIS; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; HEK293 CELL LINE; HUMAN; HUMAN CELL; IMAGING; INTERNALIZATION; JURKAT CELL LINE; MUTANT; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; RECEPTOR BINDING; U937 CELL LINE; CELL LINE; CHEMISTRY; METABOLISM;

CELL LINE; CHEMOKINE CCL2; CHEMOKINE CCL21; CHEMOKINE CXCL12; CHEMOKINES; HUMANS; RECEPTORS, CCR2; RECEPTORS, CCR7; RECEPTORS, CXCR4; RECOMBINANT PROTEINS;

EID: 84900326374     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0081454     Document Type: Article

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