G-protein stimulatory subunit alpha and Gq/11α G-proteins are both required to maintain quiescent stem-like chondrocytes

Nature Communications

Volumn 5, Issue , 2014, Pages

  Chagin, Andrei S ^a,b   Vuppalapati, Karuna K ^a   Kobayashi, Tatsuya ^b   Guo, Jun ^b   Hirai, Takao ^b   Chen, Min ^c   Offermanns, Stefan ^d   Weinstein, Lee S ^c   Kronenberg, Henry M ^b  

^a KAROLINSKA INSTITUTE   (Sweden)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

^c NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^d MAX PLANCK INSTITUTE FOR HEART AND LUNG RESEARCH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

G11ALPHA PROTEIN; GQ PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; PARATHYROID HORMONE RELATED PROTEIN RECEPTOR; UNCLASSIFIED DRUG; PARATHYROID HORMONE RECEPTOR 1;

ANIMAL EXPERIMENT; APOPTOSIS; ARTICLE; CARTILAGE CELL; CELL DIFFERENTIATION; CELL GROWTH; CELL PROLIFERATION; INTRACELLULAR SIGNALING; MOUSE; NONHUMAN; PROTEIN MODIFICATION; STEM LIKE CHONDROCYTE; TRANSGENIC MOUSE; ANIMAL; CYTOLOGY; GENETICS; GENOTYPE; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; METABOLISM; PHYSIOLOGY;

ANIMALS; APOPTOSIS; CHONDROCYTES; GENOTYPE; GTP-BINDING PROTEIN ALPHA SUBUNITS; GTP-BINDING PROTEIN ALPHA SUBUNITS, GQ-G11; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; MICE; MICE, TRANSGENIC; RECEPTOR, PARATHYROID HORMONE, TYPE 1;

EID: 84899855525     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms4673     Document Type: Article

References (29)

1. Kember, N. F. & Lambert, B. E. Slowly cycling cells in growing bone. Cell Tissue Kinet. 14, 327-330 (1981).
2. Kember, N. F. Cell division in endochondral ossification. A study of cell proliferation in rat bones by the method of tritiated thymidine autoradiography. J. Bone Joint Surg. Br. 42B, 824-839 (1960).
3. Abad, V. et al. The role of the resting zone in growth plate chondrogenesis. Endocrinology 143, 1851-1857 (2002).
4. Mackie, E. J., Tatarczuch, L. & Mirams, M. The growth plate chondrocyte and endochondral ossification. J. Endocrinol. 211, 109-121 (2011).
5. Kronenberg, H. M. Developmental regulation of the growth plate. Nature 423, 332-336 (2003).
6. Abou-Samra, A. B. et al. Expression cloning of a common receptor for parathyroid hormone and parathyroid hormone-related peptide from rat osteoblast-like cells: a single receptor stimulates intracellular accumulation of both cAMP and inositol trisphosphates and increases intracellular free calcium. Proc. Natl Acad. Sci. USA 89, 2732-2736 (1992).
7. Singh, A. T., Gilchrist, A., Voyno-Yasenetskaya, T., Radeff-Huang, J. M. & Stern, P. H. G alpha12/G alpha13 subunits of heterotrimeric G proteins mediate parathyroid hormone activation of phospholipase D in UMR-106 osteoblastic cells. Endocrinology 146, 2171-2175 (2005).
8. Bastepe, M. et al. Stimulatory G protein directly regulates hypertrophic differentiation of growth plate cartilage in vivo. Proc. Natl Acad. Sci. USA 101, 14794-14799 (2004).
9. Guo, J., Chung, U. I., Kondo, H., Bringhurst, F. R. & Kronenberg, H. M. The PTH/PTHrP receptor can delay chondrocyte hypertrophy in vivo without activating phospholipase C. Dev. Cell 3, 183-194 (2002).
10. Hirai, T., Chagin, A. S., Kobayashi, T., Mackem, S. & Kronenberg, H. M. Parathyroid hormone/parathyroid hormone-related protein receptor signaling is required for maintenance of the growth plate in postnatal life. Proc. Natl Acad. Sci. USA 108, 191-196 (2011).
11. Sakamoto, A., Chen, M., Kobayashi, T., Kronenberg, H. M. & Weinstein, L. S. Chondrocyte-specific knockout of the G protein G(s)alpha leads to epiphyseal and growth plate abnormalities and ectopic chondrocyte formation. J. Bone Miner. Res. 20, 663-671 (2005).
12. Arnold, M. A. et al. MEF2C transcription factor controls chondrocyte hypertrophy and bone development. Dev. Cell 12, 377-389 (2007).
13. Yoon, B. S. et al. Bmpr1a and Bmpr1b have overlapping functions and are essential for chondrogenesis in vivo. Proc. Natl Acad. Sci. USA 102, 5062-5067 (2005).
14. Yoon, B. S. et al. BMPs regulate multiple aspects of growth-plate chondrogenesis through opposing actions on FGF pathways. Development 133, 4667-4678 (2006).
15. Yu, S. et al. Variable and tissue-specific hormone resistance in heterotrimeric Gs protein alpha-subunit (Gsalpha) knockout mice is due to tissue-specific imprinting of the gsalpha gene. Proc. Natl Acad. Sci. USA 95, 8715-8720 (1998).
16. Iida-Klein, A. et al. Mutations in the second cytoplasmic loop of the rat parathyroid hormone (PTH)/PTH-related protein receptor result in selective loss of PTH-stimulated phospholipase C activity. J. Biol. Chem. 272, 6882-6889 (1997).
17. Guo, J. et al. Signal-selectivity of parathyroid hormone (PTH)/PTH-related peptide receptor-mediated regulation of differentiation in conditionally immortalized growth-plate chondrocytes. Endocrinology 142, 1260-1268 (2001).
18. Beier, F. et al. TGFbeta and PTHrP control chondrocyte proliferation by activating cyclin D1 expression. Mol. Biol. Cell 12, 3852-3863 (2001).
19. Kobayashi, T. et al. Indian hedgehog stimulates periarticular chondrocyte differentiation to regulate growth plate length independently of PTHrP. J. Clin. Invest. 115, 1734-1742 (2005).
20. Tai, T. C. & Wong, D. L. Protein kinase A and protein kinase C signaling pathway interaction in phenylethanolamine N-methyltransferase gene regulation. J. Neurochem. 85, 816-829 (2003).
21. Fricke, K., Heitland, A. & Maronde, E. Cooperative activation of lipolysis by protein kinase A and protein kinase C pathways in 3T3-L1 adipocytes. Endocrinology 145, 4940-4947 (2004).
22. Motzkus, D. et al. The human PER1 gene is transcriptionally regulated by multiple signaling pathways. FEBS Lett. 486, 315-319 (2000).
23. Pawson, C. T. & Scott, J. D. Signal integration through blending, bolstering and bifurcating of intracellular information. Nat. Struct. Mol. Biol. 17, 653-658 (2010).
24. Kobayashi, T. et al. PTHrP and Indian hedgehog control differentiation of growth plate chondrocytes at multiple steps. Development 129, 2977-2986 (2002).
25. Gu, J. L., Muller, S., Mancino, V., Offermanns, S. & Simon, M. I. Interaction of G alpha(12) with G alpha(13) and G alpha(q) signaling pathways. Proc. Natl Acad. Sci. USA 99, 9352-9357 (2002).
26. Moers, A. et al. G13 is an essential mediator of platelet activation in hemostasis and thrombosis. Nat. Med. 9, 1418-1422 (2003).
27. Nakamura, E., Nguyen, M. T. & Mackem, S. Kinetics of tamoxifen-regulated Cre activity in mice using a cartilage-specific CreER(T) to assay temporal activity windows along the proximodistal limb skeleton. Dev. Dyn. 235, 2603-2612 (2006).
28. Soriano, P. Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat. Genet. 21, 70-71 (1999).
29. Vega, R. B. et al. Histone deacetylase 4 controls chondrocyte hypertrophy during skeletogenesis. Cell 119, 555-566 (2004).