Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS

Journal of Proteomics

Volumn 103, Issue , 2014, Pages 194-203

  Chen, Rui ^a,b   Seebun, Deeptee ^a   Ye, Mingliang ^b   Zou, Hanfa ^b   Figeys, Daniel ^a,c  

^a UNIVERSITY OF OTTAWA   (Canada)

^b DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

^c UNIVERSITY OF OTTAWA   (Canada)

Author keywords

Glycan composition; HEK 293T; Mass spectrometry; N glycoproteome

Indexed keywords

CELL MEMBRANE PROTEIN; GLYCAN; GLYCOPEPTIDE; GUANINE NUCLEOTIDE BINDING PROTEIN; OCTOXINOL; TRITON X 100; MEMBRANE PROTEIN; POLYSACCHARIDE;

AMINO ACID SEQUENCE; ARTICLE; CELL MEMBRANE; CELL STRAIN HEK293; CHEMICAL REACTION; COLLISIONALLY ACTIVATED DISSOCIATION; GLYCOSYLATION; HIGH ENERGY COLLISION DISSOCIATION; HUMAN; HUMAN CELL; HYDROPHILIC INTERACTION CHROMATOGRAPHY; KIDNEY CELL; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION; SOLID PHASE EXTRACTION; TANDEM MASS SPECTROMETRY; CHEMICAL PHENOMENA; CHEMISTRY; HEK293 CELL LINE; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; PROCEDURES; PROTEIN PROCESSING;

CHROMATOGRAPHY, LIQUID; GLYCOPEPTIDES; GLYCOSYLATION; HEK293 CELLS; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MEMBRANE PROTEINS; POLYSACCHARIDES; PROTEIN PROCESSING, POST-TRANSLATIONAL; SOLID PHASE EXTRACTION; TANDEM MASS SPECTROMETRY;

EID: 84899549399     PISSN: 18743919     EISSN: 18767737     Source Type: Journal    
DOI: 10.1016/j.jprot.2014.03.040     Document Type: Article

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