Monitoring in vivo reversible cysteine oxidation in proteins using ICAT and mass spectrometry

Nature Protocols

Volumn 9, Issue 5, 2014, Pages 1131-1145

  García Santamarina, Sarela ^a   Boronat, Susanna ^a   Domènech, Alba ^a   Ayté, José ^a   Molina, Henrik ^b   Hidalgo, Elena ^a  

^a UNIVERSITAT POMPEU FABRA   (Spain)

^b ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; IODOACETAMIDE; STABLE ISOTOPE; PROTEIN; THIOL DERIVATIVE;

ACIDIFICATION; AFFINITY LABELING; AMINO ACID ANALYSIS; AMINO ACID METABOLISM; ARTICLE; CONTROLLED STUDY; FREEZING; FUNGAL CELL CULTURE; IN VIVO STUDY; INTERMETHOD COMPARISON; ISOTOPE CODED AFFINITY TAG; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MEASUREMENT ACCURACY; NONHUMAN; OXIDATION; OXIDATION REDUCTION REACTION; OXIDATION REDUCTION STATE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN DEGRADATION; PROTEIN PURIFICATION; PROTEOMICS; SCHIZOSACCHAROMYCES POMBE; SENSITIVITY ANALYSIS; TANDEM MASS SPECTROMETRY; METABOLISM; PROCEDURES; YEAST;

CHROMATOGRAPHY, LIQUID; CYSTEINE; ISOTOPE LABELING; MASS SPECTROMETRY; OXIDATION-REDUCTION; PROTEINS; SULFHYDRYL COMPOUNDS; TANDEM MASS SPECTROMETRY; YEASTS;

EID: 84899542656     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.065     Document Type: Article

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