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Volumn 292, Issue 5520, 2001, Pages 1360-1363

Bacterial recognition of mineral surfaces: Nanoscale interactions between Shewanella and α-FeOOH

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CHARGE TRANSFER; EARTH (PLANET); ENZYMES; FORCE MEASUREMENT; MICROSCOPIC EXAMINATION; NANOSTRUCTURED MATERIALS;

EID: 0035907067     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1059567     Document Type: Article
Times cited : (396)

References (45)
  • 22
    • 0343036853 scopus 로고    scopus 로고
    • note
    • S. oneidensis (American Type Culture Collection no. 700550) was transformed with plasmid pSMC2, which codes for a constitutive, intracellular, green fluorescent protein (44). Transformed cells were grown in an anaerobic chamber (Coy Laboratory Products, Ann Arbor, MI) to midlogarithmic phase at room temperature as previously described (4) in a defined medium supplemented with 15 mM lactate and 2 mM ferric citrate (4). 23. Glass beads (Polysciences, Warrington, PA) were cleaned with hydrogen peroxide (30% solution), rinsed with ultrapure water, and functionalized with amino groups by incubation in a solution of 3-aminopropyltriethoxysilane (5 to 10% in acetone) for 6 hours. Previous studies have shown that linker molecules such as amino-silane have no effect on the measured bacterium-mineral interactions (19, 20). Cultured cells were rinsed three times in an anaerobic solution of 10 mM sodium chloride and were linked to functionalized glass beads by spinning the cell-bead mixture at 7000g for 2 min. A single bacteria-coated bead was then attached to a cantilever with epoxy (19). Scanning laser confocal microscopy was used to verify bacterial coverage of the probe by imaging the green fluorescent signal emitted from cells (19, 20). The viability of cells was assessed by inoculating an agar plate with a biologically active force probe after the force measurements.
  • 23
    • 0343472686 scopus 로고    scopus 로고
    • note
    • It is difficult to quantify the area of contact between a force probe and a sample surface. Three-dimensional confocal microscopy measurements of biologically active force probes, as described previously (79, 20), suggest that a single bacterium made contact with the mineral during force measurements.
  • 24
    • 0342602625 scopus 로고    scopus 로고
    • note
    • The region of constant compliance was not used to determine deflection, because it would have resulted in an overestimate of the measured forces because of the fact that the cells were more compliant than the cantilever (20, 45).
  • 35
    • 0343908429 scopus 로고    scopus 로고
    • note
    • On the basis of transmission electron microscopy and freeze-substitution analyses, extracellular polysaccharide, a common macromolcule on the surface of many bacteria, has not been detected on the cell wall of 5. oneidensis (A. Korenevsky and T. Beveridge, unpublished data).
  • 42
    • 0342602624 scopus 로고    scopus 로고
    • note
    • Future studies will use biological force microscopy with mutants incapable of producing and/or secreting cell wall biomolecules such as the 150-kD protein.
  • 45
    • 0342602620 scopus 로고    scopus 로고
    • note
    • We thank B. Lower, C. Myers, J. Banfield, and the anonymous reviewers for constructive comments; G. O'Toole for providing plasmid pSMC2; and J. Tak for support. This manuscript is dedicated to B. Diehl. Mineral samples were provided by the Virginia Tech Geological Sciences Museum. Financial support was provided by the U.S. Department of Energy and the NSF.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.