Evidence of TGF-β1 mediated epithelial-mesenchymal transition in immortalized benign prostatic hyperplasia cells

Molecular Membrane Biology

Volumn 31, Issue 2-3, 2014, Pages 103-110

  Hu, Shuai ^a,b   Yu, Wei ^a,b   Lv, Tian Jing ^a,b   Chang, Chawn Shang ^c   Li, Xin ^a,b   Jin, Jie ^a,b  

^a PEKING UNIVERSITY FIRST HOSPITAL   (China)

^b Institute of Urology   (China)

^c UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

Benign prostate hyperplasia; Epithelial mesenchymal transition

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; CYTOKERATIN 5; CYTOKERATIN 8; MONOCLONAL ANTIBODY; SMAD3 PROTEIN; TRANSFORMING GROWTH FACTOR BETA1; UVOMORULIN; VIMENTIN; CULTURE MEDIUM; TGFB1 PROTEIN, HUMAN;

ARTICLE; CELL CULTURE; CELL MIGRATION; CELL STRUCTURE; EPITHELIAL MESENCHYMAL TRANSITION; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; MALE; MICROENVIRONMENT; PRIORITY JOURNAL; PROSTATE HYPERTROPHY; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; SPINDLE CELL; SUPERNATANT; UPREGULATION; WESTERN BLOTTING; ANTAGONISTS AND INHIBITORS; CELL LINE; CELL MOTION; CULTURE MEDIUM; CYTOLOGY; DRUG EFFECTS; GENE EXPRESSION REGULATION; METABOLISM; PATHOLOGY; PHARMACOLOGY; SIGNAL TRANSDUCTION; STROMA CELL; TUMOR MICROENVIRONMENT;

ANTIBODIES, MONOCLONAL; CELL LINE; CELL MOVEMENT; CELLULAR MICROENVIRONMENT; CULTURE MEDIA, CONDITIONED; EPITHELIAL-MESENCHYMAL TRANSITION; GENE EXPRESSION REGULATION; HUMANS; MALE; PROSTATIC HYPERPLASIA; SIGNAL TRANSDUCTION; STROMAL CELLS; TRANSFORMING GROWTH FACTOR BETA1;

EID: 84899450068     PISSN: 09687688     EISSN: 14645203     Source Type: Journal    
DOI: 10.3109/09687688.2014.894211     Document Type: Article

References (23)

1. Alonso-Magdalena P, Brössner C, Reiner A, Cheng G, Sugiyama N. 2009. A role for epithelial-mesenchymal transition in the etiology of benign prostatic hyperplasia. Proc Natl Acad Sci USA 106: 2859-2863.
2. Ao M, Williams K, Bhowmick N, Hayward S. 2006. Transforming growth factor-{beta} promotes invasion in tumorigenic but not in nontumorigenic human prostatic epithelial cells. Cancer Res 66: 8007-8016.
3. Comijn J, Berx G, Vermassen P, Verschueren K, van Grunsven L. 2001. The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion. Mol Cell 7: 1267-1278.
4. Cunha GR. 1994. Role of mesenchymal-epithelial interactions in normal and abnormal development of the mammary gland and prostate. Cancer 74: 1030-1044.
5. Gerdes MJ, Larsen M, Dang TD, Ressler SJ, Tuxhorn JA. 2004. Regulation of rat prostate stromal cell myodifferentiation by androgen and TGF-b1b1. Prostate 58: 299-307.
6. Guarino M. 1995. Epithelial-to-mesenchymal change of differentiation. From embryogenetic mechanism to pathological patterns. Histol Histopathol 10: 171-184.
7. Huang X, Lee C. 2003. Regulation of stromal proliferation, growth arrest, differentiation and apoptosis in benign prostatic hyperplasia by TGF-b1beta. Front Biosci 8:s740-s749.
8. Lu T, Lin WJ, Izumi K. 2012. Targeting androgen receptor to suppress macrophage-induced EMT and benign prostatic hyperplasia (BPH) development. Mol Endocrinol 26: 1707-1715.
9. Kalluri R, Weinberg RA. 2009. The basics of epithelial-mesenchymal transition. J Clin Invest 119: 1420-1428.
10. Massagué J, Seoane J, Wotton D. 2005. Smad transcription factors. Genes Dev 19: 2783-2810.
11. Moustakas A, Heldin CH. 2007. Signaling networks guiding epithelialmesenchymal transitions during embryogenesis and cancer progression. Cancer Sci 98: 1512-1520.
12. Rohr HP, Bartsch G. 1980. Human benign prostatic hyperplasia: A stromal disease? New perspectives by quantitative morphology. Urology 16: 625-633.
13. Siejka A, Schally AV, Barabutis N. 2014. The effect of LHRH antagonist cetrorelix in crossover conditioned media from epithelial (BPH-1) and stromal (WPMY-1) prostate cells. Horm Metab Res 46: 21-26.
14. Sjöblom T, Jones S, Wood LD, Parsons DW, Lin J. 2006. The consensus coding sequences of human breast and colorectal cancers. Science 314: 268-274.
15. Slabáková E, Pernicová Z, Slavíč ková E, Staršíchová A, Kozubík A, Souček K. 2011. TGF- beta 1-induced EMT of non-transformed prostate hyperplasia cells is characterized by early induction of SNAI2/Slug. The Prostate 70: 1332-1343.
16. Tuxhorn JA, Ayala GE, Smith MJ, Smith VC, Dang TD, Rowley DR. 2002. Reactive stroma in human prostate cancer induction of myofibroblast phenotype and extracellular matrix remodeling. Clin Cancer Res 8: 2912-2923.
17. Untergasser G, Madersbacher S, Berger P. 2005. Benign prostatic hyperplasia: age-related tissue-remodeling. Exp Gerontol 40: 121-128.
18. van der Pluijm G. 2011. Epithelial plasticity, cancer stem cells and bone metastasis formation. Bone 48: 37-43.
19. Voulgari A, Pintzas A. 2009. Epithelial-mesenchymal transition in cancer metastasis: Mechanisms, markers and strategies to overcome drug resistance in the clinic. Biochim Biophys Acta 1796: 75-90.
20. Webber MM, Trakul N, Thraves PS, Bello-DeOcampo D, Chu WW. 1999. A human prostatic stromal myofibroblast cell line WPMY-1: A model for stromal-epithelial interactions in prostatic neoplasia. Carcinogenesis 20: 1185-1192.
21. Wong YC, Yu SQ, Wang XH, Han BM, Zhao FJ. 2011. Differences in phenotype and gene expression of prostate stromal cells from patients of varying ages and their influence on tumour formation by prostate epithelial cells. Asian J Androl 6: 1-10.
22. Zavadil J, Bottinger EP. 2005. TGF-b1beta and epithelialto- mesenchymal transitions. Oncogene 24: 5764-5774.
23. Zeisberg M, Neilson EG. 2009. Biomarkers for epithelial-mesenchymal transitions. J Clin Invest 119: 1429-1437.