NhaA Na+/H+ antiporter mutants that hardly react to the membrane potential

PLoS ONE

Volumn 9, Issue 4, 2014, Pages

  Alkoby, Dudu ^a   Rimon, Abraham ^a   Burdak, Maral ^b   Patino Ruiz, Miyer ^b   Cǎlinescu, Octavian ^b   Fendler, Klaus ^b   Padan, Etana ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

LITHIUM ION; MUTANT PROTEIN; NHAA PROTEIN; PROTEIN VARIANT; SODIUM ION; SODIUM PROTON EXCHANGE PROTEIN; UNCLASSIFIED DRUG; ESCHERICHIA COLI PROTEIN;

ARTICLE; CATION EXCHANGE; CELL MEMBRANE POTENTIAL; CELL PH; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CRYSTAL STRUCTURE; ELECTROCHEMICAL ANALYSIS; ESCHERICHIA COLI; MOLECULAR LIBRARY; NONHUMAN; PHENOTYPE; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN ISOLATION; STOICHIOMETRY; WILD TYPE; CELL MEMBRANE; CHEMISTRY; ELECTROPHYSIOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; KINETICS; MEMBRANE POTENTIAL; METABOLISM; MUTATION; PH; PHYSIOLOGY; PROTEIN CONFORMATION; SITE DIRECTED MUTAGENESIS;

CELL MEMBRANE; ELECTROPHYSIOLOGY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HYDROGEN-ION CONCENTRATION; KINETICS; MEMBRANE POTENTIALS; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; MUTATION; PROTEIN CONFORMATION; SODIUM-HYDROGEN ANTIPORTER;

EID: 84899411188     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0093200     Document Type: Article

References (46)

1. Krulwich TA, Sachs G, Padan E (2011) Molecular aspects of bacterial pH sensing and homeostasis. Nature reviews Microbiology 9: 330-343.
2. Padan E, Kozachkov L, Herz K, Rimon A (2009) NhaA crystal structure: functional-structural insights. J Exp Biol 212: 1593-1603.
3. +/ homeostasis in cells. Bioch Biophys Acta (In press).
4. Fliegel L (2008) Molecular biology of the myocardial Na+/H+ exchanger. J Mol Cell Cardiol 44: 228-237.
5. Padan E, Bibi E, Masahiro I, Krulwich TA (2005) Alkaline pH homeostasis in bacteria: New insights. Biochim Biophys Acta 1717: 67-88. (Pubitemid 41682429)
6. + antiporters. Biochim Biophys Acta 1505: 144-157.
7. Brett CL, Donowitz M, Rao R (2005) Evolutionary origins of eukaryotic sodium/proton exchangers. Am J Physiol Cell Physiol 288: C223-239. (Pubitemid 40105332)
8. + antiporter coded by nhaA (ant) from Escherichia coli. J Biol Chem 266: 11289-11294.
9. Taglicht D, Padan E, Schuldiner S (1993) Proton-sodium stoichiometry of NhaA, an electrogenic antiporter from Escherichia coli. J Biol Chem 268: 5382-5387. (Pubitemid 23090853)
10. Orlowski J, Grinstein S (2004) Diversity of the mammalian sodium/proton exchanger SLC9 gene family. Pflugers Arch 447: 549-565. (Pubitemid 38241440)
11. Orlowski J, Grinstein S (2007) Emerging roles of alkali cation/proton exchangers in organellar homeostasis. Current opinion in cell biology 19: 483-492. (Pubitemid 47271963)
12. Putney LK, Denker SP, Barber DL (2002) The changing face of the Na+/H+ exchanger, NHE1: structure, regulation, and cellular actions. Annu Rev Pharmacol Toxicol 42: 527-552. (Pubitemid 34160535)
13. + exchangers by measurement of reverse mode reaction. J Biol Chem 278: 43580-43585.
14. Zuber D, Krause R, Venturi M, Padan E, Bamberg E, et al. (2005) Kinetics of charge translocation in the passive downhill uptake mode of the Na +/H+ antiporter NhaA of Escherichia coli. Biochim Biophys Acta 1709: 240-250. (Pubitemid 41254217)
15. Mager T, Rimon A, Padan E, Fendler K (2011) Transport mechanism and pH regulation of the Na +/H+ antiporter NhaA from Escherichia coli: an electrophysiological study. J Biol Chem 286: 23570-23581.
16. Mager T (2013) Differential effect of mutations on the transport properties of NhaA from Escherichia coli. J Biol Chem 288: 24666-24675.
17. Jardetzky O (1996) Protein dynamics and conformational transitions in allosteric proteins. Progress in biophysics and molecular biology 65: 171-219.
18. Mitchell P (1968) Chemiosmotic coupling and energy transduction (Glynn Research Ltd, Bodmin, England).
19. + antiporter and insights into mechanism of action and regulation by pH. Nature 534: 1197-1202.
20. Padan E (2008) The enlightening encounter between structure and function in the NhaA Na(+)-H(+) antiporter. Trends Biochem Sci 33: 435-443.
21. Krishnamurthy H, Piscitelli CL, Gouaux E (2009) Unlocking the molecular secrets of sodium-coupled transporters. Nature 459: 347-355.
22. Boudker O, Verdon G (2010) Structural perspectives on secondary active transporters. Trends in pharmacological sciences 31: 418-426.
23. Shi Y (2013) Common folds and transport mechanisms of secondary active transporters. Ann Rev Biophys 42: 51-72.
24. Tzubery T, Rimon A, Padan E (2008) Structure-based functional study reveals multiple roles of transmembrane segment IX and loop VIII-IX in NhaA Na+/H+ antiporter of Escherichia coli at physiological pH. J Biol Chem 283: 15975-15987.
25. Maes M, Rimon A, Kozachkov-Magrisso L, Friedler A, Padan E (2012) Revealing the Ligand Binding Site of NhaA Na+/H+ Antiporter and its pH Dependence. J Biol Chem 287: 38150-38157.
26. Radchenko MV, Tanaka K, Waditee R, Oshimi S, Matsuzaki Y, et al. (2006) Potassium/proton antiport system of Escherichia coli. J Biol Chem 281: 19822-19829. (Pubitemid 44065756)
27. + antiporter in Escherichia coli . J Biol Chem 268: 1729-1734.
28. + antiporter as well as the growth phenotype of Escherichia coli. J Biol Chem 273: 26470-26476.
29. Galili L, Herz K, Dym O, Padan E (2004) Unraveling functional and structural interactions between transmembrane domains IV and XI of NhaA Na+/H+ antiporter of Escherichia coli. J Biol Chem 279: 23104-23113. (Pubitemid 38685615)
30. + antiporter of Escherichia coli . Biochemistry 41: 609-617.
31. Schulz P, Garcia-Celma JJ, Fendler K (2008) SSM-based electrophysiology. Methods 46: 97-103.
32. Nozaki K, Kuroda T, Mizushima T, Tsuchiya T (1998) A new Na+/H+ antiporter, NhaD, of Vibrio parahaemolyticus. Biochim Biophys Acta 1369: 213-220. (Pubitemid 28132858)
33. + antiporter of Escherichia coli . J Biol Chem 279: 3265-3272.
34. Padan E, Zilberstein D, Rottenberg H (1976) The proton electrochemical gradient in Escherichia coli cells. European journal of biochemistry/FEBS 63: 533-541.
35. Kozachkov L, Herz K, Padan E (2007) Functional and structural interactions of the transmembrane domain X of NhaA, Na+/H+ antiporter of Escherichia coli, at physiological pH. Biochemistry 46: 2419-2430. (Pubitemid 46362416)
36. Rimon A, Kozachkov-Magrisso L, Padan E (2012) The unwound portion dividing helix IV of NhaA undergoes a conformational change at physiological pH and lines the cation passage. Biochemistry 51: 9560-9569.
37. + antiporter of Escherichia coli is exposed and faces the cell exterior. J Biol Chem 272: 1761-1768.
38. Ohyama T, Igarashi K, Kobayashi H (1994) Physiological role of the chaA gene in sodium and calcium circulations at a high pH in Escherichia coli. J Bacteriol 176: 4311-4315. (Pubitemid 24226468)
39. Padan E, Maisler N, Taglicht D, Karpel R, Schuldiner S (1989) Deletion of ant in Escherichia coli reveals its function in adaptation to high salinity and an alternative Na+/H+ antiporter system(s). J Biol Chem 264: 20297-20302. (Pubitemid 20009210)
40. Davies B, and Mingioli E. (1950) Mutants of Escherichia Coli requiring methionine or vitamin B12. J Bacteriol 60: 17-28.
41. Rosen BP (1986) Ion extrusion systems in E. coli. Methods Enzymol 125: 328-386.
42. + antiporter gene of Escherichia coli. Proc Natl Acad Sci USA 84: 2615-2619.
43. Schuldiner S, Fishkes H (1978) Sodium-proton antiport in isolated membrane vesicles of Escherichia coli. Biochemistry 17: 706-711. (Pubitemid 8295115)
44. + antiporter (NhaA) protein from Helicobacter pylori required for ion transport and pH sensing. J Biol Chem 278: 21467-21473.
45. Pinner E, Padan E, Schuldiner S (1994) Kinetic properties of NhaB, a Na+/H+ antiporter from Escherichia coli. J Biol Chem 269: 26274-26279. (Pubitemid 24328444)
46. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.