Mutation E252C Increases Drastically the Km Value for Na + and Causes an Alkaline Shift of the pH Dependence of NhaA Na +/H+ Antiporter of Escherichia coli

Journal of Biological Chemistry

Volumn 279, Issue 5, 2004, Pages 3265-3272

  Tzubery, Tzvi ^a   Rimon, Abraham ^a   Padan, Etana ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI; PH EFFECTS; PROTEINS; SODIUM;

ANTIPORTER;

BIOCHEMISTRY;

ANTIPORTER; BACTERIAL PROTEIN; CYSTEINE; GLUTAMINE; LIGAND; MEMBRANE PROTEIN; N ETHYLMALEIMIDE; NHAA PROTEIN; PROTON; SODIUM; SODIUM PROTON EXCHANGE PROTEIN; THIOMESYLIC ACID METHYL ESTER; TRYPSIN;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; CHEMICAL MODIFICATION; CHEMISTRY; CONFORMATIONAL TRANSITION; CROSS LINKING; CRYOELECTRON MICROSCOPY; CRYSTAL STRUCTURE; DOSE RESPONSE; E252C GENE; ESCHERICHIA COLI; GENE; GENE MUTATION; GENETICS; KINETICS; LIGAND BINDING; METABOLISM; MUTATION; NONHUMAN; OLIGOMERIZATION; PH; PHYSIOLOGY; PLASMID; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN SECONDARY STRUCTURE; PROTEOLIPOSOME; SITE DIRECTED MUTAGENESIS;

ANTIPORTERS; BACTERIAL PROTEINS; CELL MEMBRANE; CYSTEINE; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HYDROGEN-ION CONCENTRATION; KINETICS; LIGANDS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PLASMIDS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTONS; SODIUM; SODIUM-HYDROGEN ANTIPORTER; TRYPSIN;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0942298125     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M309021200     Document Type: Article

References (54)

1. Padan, Padail, E., and Schuldiner, S. (1996) in The Handbook of Biological Physics (Konings, W. N., Kaback, H. R., and Lolkema, J., eds) Vol. II, pp. 501-531, Elsevier Science, The Netherlands
2. Padan, E., and Krulwich, T. A. (2000) in Bacterial Stress Responses (Storz, G., and Hengge-Aronis, R., eds) pp. 117-130, ASM Press, Washington, D. C.
3. Padan, E., Venturi, M., Gerchman, Y., and Dover, N. (2001) Biochim. Biophys. Acta 1505, 144-157
4. Padan, E., Maisler, N., Taglicht, D., Karpel, R., and Schuldiner, S. (1989) J. Biol. Chem. 264, 20297-20302
5. Pinner, E., Kotler, Y., Padan, E., and Schuldiner, S. (1993) J. Biol. Chem. 268, 1729-1734
6. Taglicht, D., Padan, E., and Schuldiner, S. (1991) J. Biol. Chem. 266, 11289-11294
7. Taglicht, D., Padan, E., and Schuldiner, S. (1993) J. Biol. Chem. 268, 5382-5387
8. Venturi, M., and Padan, E. (2002) in A Practical Guide to Membrane Protein Purification (Hunte, C., Von Jagow, G., and Schagger, H., eds) 2nd Ed., pp. 179-190, Academic Press, Amsterdam, The Netherlands
9. Rothman, A., Padan, E., and Schuldiner, S. (1996) J. Biol. Chem. 271, 32288-32292
10. Olami, Y., Rimon, A., Gerchman, Y., Rothman, A., and Padan, E. (1997) J. Biol. Chem. 272, 1761-1768
11. Williams, K. A., Geldmacher-Kaufer, U., Padan, E., Schuldiner, S., and Kuhlbrandt, W. (1999) EMBO J. 18, 3558-3563
12. Gerchman, Y., Rimon, A., Venturi, M., and Padan, E. (2001) Biochemistry 40, 3403-3412
13. Williams, K. A. (2000) Nature 403, 112-115
14. Gerchman, Y., Olami, Y., Rimon, A., Taglicht, D., Schuldiner, S., and Padan, E. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 1212-1216
15. Rimon, A., Gerchman, Y., Olami, Y., Schuldiner, S., and Padan, E. (1995) J. Biol. Chem. 270, 26813-26817
16. Rimon, A., Gerchman, Y., Kariv, Z., and Padan, E. (1998) J. Biol. Chem. 273, 26470-26476
17. Padan, E., Venturi, M., Michel, H., and Hunte, C. (1998) FEBS Lett. 441, 53-58
18. Venturi, M., Rimon, A., Gerchman, Y., Hunte, C., Padan, E., and Michel, H. (2000) J. Biol. Chem. 275, 4734-4742
19. Gerchman, Y., Rimon, A., and Padan, E. (1999) J. Biol. Chem. 274, 24617-24624
20. Davies, B., and Mingioli, E. (1950) J. Bacteriol. 60, 17-28
21. Galili, L., Rothman, A., Kozachkov, L., Rimon, A., and Padan, E. (2002) Biochemistry 41, 609-617
22. Rimon, A., Tzubery, T., Galili, L., and Padan, E. (2002) Biochemistry 41, 14897-14905
23. Ho, S., Hunt, H., Horton, R., Pullen, J., and Pease, L. (1989) Gene (Amst.) 77, 51-59
24. Rosen, B. (1986) Methods Enzymol. 125, 328-336
25. Goldberg, E. B., Arbel, T., Chen, J., Karpel, R., Mackie, G. A., Schuldiner, S., and Padan, E. (1987) Proc. Natl. Acad. Sci. U. S. A. 84, 2615-2619
26. Bradford, M. (1976) Anal. Biochem. 72, 248-254
27. Laemmli, U. (1970) Nature 227, 680-685
28. Rothman, A., Gerchman, Y., Padan, E., and Schuldiner, S. (1997) Biochemistry 36, 14572-14576
29. Tamura, N., Konishi, S., Iwaki, S., Kimura-Someya, T., Nada, S., and Yamaguchi, A. (2001) J. Biol. Chem. 276, 20330-20339
30. Kaback, H. R., Sahin-Toth, M., and Weinglass, A. B. (2001) Nat. Rev. Mol. Cell. Biol. 2, 610-620
31. Angevine, C. M., and Fillingame, R. H. (2003) J. Biol. Chem. 278, 6066-6074
32. Akabas, M., Kaufmann, C., Archdeacon, P., and Karlin, A. (1994) Neuron 13, 919-927
33. Akabas, M., Stauffer, D., Xu, M., and Karlin, A. (1992) Science 258, 307-310
34. Gorin, G., Martic, P. A., and Doughty, G. (1966) Arch. Biochem. Biophys. 115, 593-597
35. Roberts, D. D., Lewis, S. D., Ballou, D. P., Olson, S. T., and Shafer, J. A. (1986) Biochemistry 25, 5595-5601
36. Abdel-Dayem, M., Basquin, C., Pourcher, T., Cordat, E., and Leblanc, G. (2003) J. Biol. Chem. 278, 1518-1524
37. Li, J., Xu, Q., Cortes, D. M., Perozo, E., Laskey, A., and Karlin, A. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 11605-11610
38. Gupte, S. S., and Lane, L. K. (1979) J. Biol. Chem. 254, 10362-10369
39. Wu, J., Frillingos, S., Voss, J., and Kaback, H. R. (1994) Protein Sci. 3, 2294-2301
40. Haugland, R. P. (1983) Excited State of Biopolymers (Steiner, R. F., ed) pp. 29-58, Plenum Press, New York
41. Padan, E., Rimon, A., Tzubery, T., Muller, M., Herz, K., and Galili, L. (2003) in The Sodium-Hydrogen Exchange, from Molecule to its Role in Disease (Karmazyn, M., Avkiran, M., and Fliegel, L., eds) pp. 91-108, Kluwer Academic Publishers, Boston
42. Grinstein, S., Woodside, M., Sardet, C., Pouyssegur, J., and Rotin, D. (1992) J. Biol. Chem. 267, 23823-23828
43. Putney, L. K., Denker, S. P., and Barber, D. L. (2002) Annu. Rev. Pharmacol. Toxicol. 42, 527-552
44. Wakabayashi, S., Pang, T., Hisamitsu, T., and Shigekawa, M. (2003) in The Sodium-Hydrogen Exchange, from Molecule to its Role in Disease (Karmazyn, M., Avkiran, M., and Fliegel, L., eds) pp. 35-49, Kluwer Academic Publishers, Boston
45. Noumi, T., Inoue, H., Sakurai, T., Tsuchiya, T., and Kanazawa, H. (1997) J. Biochem. (Tokyo) 121, 661-670
46. Inoue, H., Tsuboi, Y., and Kanazawa, H. (2001) J. Biochem. (Tokyo) 129, 569-576
47. Tsuboi, Y., Inoue, H., Nakamura, N., and Kanazawa, H. (2003) J. Biol. Chem. 278, 21467-21473
48. Gupte, S. S., and Lane, L. K. (1983) J. Biol. Chem. 258, 5005-5012
49. Bigelow, D. J., and Inesi, G. (1991) Biochemistry 30, 2113-2125
50. Phillips, W. J., and Cerione, R. A. (1991) J. Biol. Chem. 266, 11017-11024
51. May, J. M., and Beechem, J. M. (1993) Biochemistry 32, 2907-2915
52. Haugland, R. P. (1989) Handbook of Fluorescent Probes and Research Chemicals, Molecular Probes Inc., Eugene, OR
53. Wu, J., Frillingos, S., and Kaback, H. (1995) Biochemistry 34, 8257-8263
54. Grunewald, M., Menaker, D., and Kanner, B. I. (2002) J. Biol. Chem. 277, 26074-26080