Label-free morphology-based prediction of multiple differentiation potentials of human mesenchymal stem cells for early evaluation of intact cells

PLoS ONE

Volumn 9, Issue 4, 2014, Pages

  Sasaki, Hiroto ^a   Takeuchi, Ichiro ^b   Okada, Mai ^a   Sawada, Rumi ^c   Kanie, Kei ^a   Kiyota, Yasujiro ^d   Honda, Hiroyuki ^a   Kato, Ryuji ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b NAGOYA INSTITUTE OF TECHNOLOGY   (Japan)

^c NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

^d NIKON CORPORATION   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ARTICLE; CASE REPORT; CD146 GENE; CD49 GENE; CDKN1A GENE; CDKN2A GENE; CELL DIFFERENTIATION; CELL LABELING; CELL POPULATION; CONTROLLED STUDY; CYTOSKELETON; FEMALE; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; MALE; MESENCHYMAL STEM CELL; PREDICTION; PTK2 GENE; STEM CELL EXPANSION; YOUNG ADULT; BIOLOGICAL MODEL; CYTOLOGY; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STROMA CELL; PHYSIOLOGY; PROCEDURES; REGENERATIVE MEDICINE;

CELL DIFFERENTIATION; GENE EXPRESSION PROFILING; HUMANS; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STROMAL CELLS; MODELS, BIOLOGICAL; REGENERATIVE MEDICINE;

EID: 84899073664     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0093952     Document Type: Article

References (43)

1. Arthur A, Zannettino A, Gronthos S (2009) The therapeutic applications of multipotential mesenchymal/stromal stem cells in skeletal tissue repair. J Cell Physiol 218: 237-245.
2. Braude P, Minger S, Warwick R (2005) Stem cell therapy: hope or hype? Safety and quality must be assured before this treatment can really benefit patients. Br Med J 330: 1159-1160.
3. Wang Y, Han ZB, Song YP, Han ZC (2012) Safety of mesenchymal stem cells for clinical application. Stem Cells Int 2012: 652034.
4. Werbowetski-Ogilvie T, Bosse M, Stewart M, Schnerch A, Ramos-Mejia V, et al. (2009) Characterization of human embryonic stem cells with features of neoplastic progression. Nat Biotechnol 27: 91-97.
5. Settleman J (2004) Tension precedes commitment - even for a stem cell. Mol Cell 14: 148-150.
6. Docheva D, Padula D, Popov C, Mutschler W, Clausen-Schaumann H, et al. (2008) Researching into the cellular shape, volume and elasticity of mesenchymal stem cells, osteoblasts and osteosarcoma cells by atomic force microscopy. J Cell Mol Med 12: 537-552. (Pubitemid 351555133)
7. Zhang L, Yu J, Pan H, Hu P, Hao Y, et al. (2007) Small molecule regulators of autophagy identified by an image-based high-throughput screen. Proc Natl Acad Sci USA 104: 19023-19028.
8. Carpenter A, Jones T, Lamprecht M, Clarke C, Kang I, et al. (2006) CellProfiler: image analysis software for identifying and quantifying cell phenotypes. Genome Biol 7: R100.
9. Futamura Y, Kawatani M, Kazami S, Tanaka K, Muroi M, et al. (2012) Morphobase, an encyclopedic cell morphology database, and its use for drug target identification. Chem Biol 19: 1620-1630.
10. Misselwitz B, Strittmatter G, Periaswamy B, Schlumberger MC, Rout S, et al. (2010) Enhanced CellClassifier: a multi-class classification tool for microscopy images. BMC Bioinformatics 11.
11. Treiser MD, Yang EH, Gordonov S, Cohen DM, Androulakis IP, et al. (2010) Cytoskeleton-based forecasting of stem cell lineage fates. Proc Natl Acad SciUS A 107: 610-615.
12. Kim JJ, Vega SL, Moghe PV (2013) A high content imaging-based approach for classifying cellular phenotypes. Methods in molecular biology 1052: 41-48.
13. Vega SL, Liu E, Patel PJ, Kulesa AB, Carlson AL, et al. (2012) High-content imaging-based screening of microenvironment-induced changes to stem cells. J Biomol Screen 17: 1151-1162.
14. Seiler C, Gazdhar A, Reyes M, Benneker LM, Geiser T, et al. (2012) Time-lapse microscopy and classification of 2D human mesenchymal stem cells based on cell shape picks up myogenic from osteogenic and adipogenic differentiation. J Tissue Eng Regen Med.
15. Friedenstein A, Gorskaja U, Kulagina N (1976) Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp Hematol 4: 267-274.
16. Caplan AI, Bruder SP (2001) Mesenchymal stem cells: building blocks for molecular medicine in the 21st century. Trends in molecular medicine 7: 259-264. (Pubitemid 32545522)
17. Matsuoka F, Takeuchi I, Agata H, Kagami H, Shiono H, et al. (2013) Morphology-based prediction of osteogenic differentiation potential of human mesenchymal stem cells. PloS ONE 8: e55082.
18. Konstantinov B, Zhou W, Golini F, Hu W-S (1994) Expert-system in the control of animal-cell culture processes - potentials, functions, and perspectives. Cytotechnology 14: 233-246.
19. Røsland GV, Svendsen A, Torsvik A, Sobala E, McCormack E, et al. (2009) Long-term cultures of bone marrow derived human mesenchymal stem cells frequeantly undergo spontaneous malignant transformation. Cancer Res 69: 5331-5339.
20. Ito T, Sawada R, Fujiwara Y, Tsuchiya T (2008) FGF-2 increases osteogenic and chondrogenic differentiation potentials of human mesenchymal stem cells by inactivation of TGF-beta signaling. Cytotechnology 56: 1-7.
21. Wang DJ, Park JS, Chu JSF, Krakowski A, Luo KX, et al. (2004) Proteomic profiling of bone marrow mesenchymal stem cells upon transforming growth factor beta 1 stimulation. J Biol Chem 279: 43725-43734. (Pubitemid 39390677)
22. Massague J, Xi Q (2012) TGF-beta control of stem cell differentiation genes. FEBS letters 586: 1953-1958.
23. Kinkel A, Fernyhough M, Helterline D, Vierck J, Oberg K, et al. (2004) Oil red-O stains non-adipogenic cells: a precautionary note. Cytotechnology 46: 49-56. (Pubitemid 40897995)
24. Gregory C, Gunn W, Peister A, Prockop D (2004) An Alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction. Anal Biochem 329: 77-84. (Pubitemid 38596903)
25. Jordan P, Brazao R, Boavida MG, Gespach C, Chastre E (1999) Cloning of a novel human Rac1b splice variant with increased expression in colorectal tumors. Oncogene 18: 6835-6839.
26. Jaffe AB, Hall A (2005) Rho GTPases: biochemistry and biology. Annu Rev Cell Dev Biol 21: 247-269. (Pubitemid 41740635)
27. McBeath R, Pirone D, Nelson C, Bhadriraju K, Chen C (2004) Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell 6: 483-495. (Pubitemid 38456178)
28. Zuckerman V, Wolyniec K, Sionov R, Haupt S, Haupt Y (2009) Tumour suppression by p53: the importance of apoptosis and cellular senescence. J Pathol 219: 3-15.
29. Motaln H, Schichor C, Lah TT (2010) Human mesenchymal stem cells and their use in cell-based therapies. Cancer 116: 2519-2530.
30. Ong CC, Jubb AM, Haverty PM, Zhou W, Tran V, et al. (2011) Targeting p21-activated kinase 1 (PAK1) to induce apoptosis of tumor cells. Proc Natl Acad Sci USA 108: 7177-7182.
31. Comer K, Dennis P, Armstrong L, Catino J, Kastan M, et al. (1998) Human smooth muscle alpha-actin gene is a transcriptional target of the p53 tumor suppressor protein. Oncogene 16: 1299-1308. (Pubitemid 28138202)
32. Weissbach L, Settleman J, Kalady M, Snijders A, Murthy A, et al. (1994) Identification of a humana RASGAP-related protein containing calmodulin-binding motifs. J Biol Chem 269: 20517-20521.
33. Jacobs J, Kieboom K, Marino S, DePinho R, van Lohuizen M (1999) The oncogene and polycomb-group gene bmi-1 regulates cell proliferation and senescence through the ink4a locus. Nature 397: 164-168. (Pubitemid 29050921)
34. Janzen V, Forkert R, Fleming HE, Saito Y, Waring MT, et al. (2006) Stem-cell ageing modified by the cyclin-dependent kinase inhibitor p16(INK4a). Nature 443: 421-426. (Pubitemid 44477274)
35. Shibata KR, Aoyama T, Shima Y, Fukiage K, Otsuka S, et al. (2007) Expression of the p16INK4A gene is associated closely with senescence of human mesenchymal stem cells and is potentially silenced by DNA methylation during in vitro expansion. Stem Cells 25: 2371-2382. (Pubitemid 47480497)
36. Bin Noh H, Ahn H, Lee W, Kwack K, Do Kwon Y (2010) The molecular signature of in vitro senescence in human mesenchymal stem cells. Genes Genomics 32: 87-93.
37. Ito T, Sawada R, Fujiwara Y, Seyama Y, Tsuchiya T (2007) FGF-2 suppresses cellular senescence of human mesenchymal stem cells by down-regulation of TGF-beta 2. Biochem Biophys Res Commun 359: 108-114. (Pubitemid 46836675)
38. Chen CS, Mrksich M, Huang S, Whitesides GM, Ingber DE (1997) Geometric control of cell life and death. Science 276: 1425-1428. (Pubitemid 27235413)
39. Roskelley CD, Desprez PY, Bissell MJ (1994) Extracellular Matrix-Dependent Tissue-Specific Gene-Expression in Mammary Epithelial-Cells Requires Both Physical and Biochemical Signal-Transduction. Proc Natl Acad Sci USA 91: 12378-12382.
40. Watt FM, Jordan PW, Oneill CH (1988) Cell-Shape Controls Terminal Differentiation of Human Epidermal-Keratinocytes. Proc Natl Acad Sci USA 85: 5576-5580.
41. Steedman H (1950) Alcian blue 8 GS - A new stain for mucin. Q J Microsc Sci 91: 477-479.
42. Ben Azouna N, Jenhani F, Regaya Z, Berraeis L, Ben Othman T, et al. (2012) Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serum. Stem Cell Res Ther 3: 6-10.
43. Tibshirani R (1996) Regression shrinkage and selection via the Lasso. J Roy Stat Soc Series B Stat Methodol 58: 267-288.