Donor substrate promiscuity of the N-acetylglucosaminyltransferase activities of Pasteurella multocida heparosan synthase 2 (PmHS2) and Escherichia coli K5 KfiA

Applied Microbiology and Biotechnology

Volumn 98, Issue 3, 2014, Pages 1127-1134

  Li, Yanhong ^a   Yu, Hai ^a   Thon, Vireak ^a,b   Chen, Yi ^a   Muthana, Musleh M ^a   Qu, Jingyao ^a   Hie, Liana ^a,c   Chen, Xi ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Escherichia coli K5 KfiA; GlcNAc transferase; Heparosan synthase; Pasteurella multocida; PmHS2; Substrate specificity; UDP GlcNAc; UDP sugars

Indexed keywords

CLONE CELLS; ESCHERICHIA COLI; HYDRAULIC STRUCTURES; OLIGOSACCHARIDES; PROTEINS; SUBSTRATES; SULFUR COMPOUNDS;

GLCNAC TRANSFERASE; PASTEURELLA MULTOCIDA; PMHS2; SUBSTRATE SPECIFICITY; SYNTHASES; UDP-GLCNAC;

POTASSIUM COMPOUNDS;

BACTERIAL ENZYME; GLYCOSAMINOGLYCAN POLYSULFATE; HEPAROSAN SYNTHASE 2; HYBRID PROTEIN; N ACETYLGLUCOSAMINE; N ACETYLGLUCOSAMINYLTRANSFERASE; SYNTHETASE; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE; URIDINE DIPHOSPHATE N ACETYLGALACTOSAMINE; ESCHERICHIA COLI PROTEIN; GLYCOSYLTRANSFERASE; HEPAROSAN SYNTHASE; KFIA PROTEIN, E COLI; N-ACETYLLACTOSAMINIDE BETA-1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE;

BACTERIUM; BIOACTIVITY; CARBOHYDRATE; ENZYME ACTIVITY; GENE EXPRESSION; SUBSTRATE; SUGAR;

AFFINITY CHROMATOGRAPHY; ARTICLE; CLONING; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PASTEURELLA MULTOCIDA; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SULFATION; ENZYMOLOGY; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR CLONING;

ESCHERICHIA COLI; PASTEURELLA MULTOCIDA;

CLONING, MOLECULAR; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; GLYCOSYLTRANSFERASES; N-ACETYLGLUCOSAMINYLTRANSFERASES; PASTEURELLA MULTOCIDA; SUBSTRATE SPECIFICITY;

EID: 84898917352     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-4947-1     Document Type: Article

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