Structure/function analysis of Pasteurella multocida heparosan synthases: Toward defining enzyme specificity and engineering novel catalysts

Journal of Biological Chemistry

Volumn 287, Issue 10, 2012, Pages 7203-7212

  Otto, Nigel J ^a   Green, Dixy E ^a   Masuko, Sayaka ^c   Mayer, Alain ^b   Tanner, Martin E ^b   Linhardt, Robert J ^c   DeAngelis, Paul L ^a  

^a UNIVERSITY OF OKLAHOMA COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c Research Rensselaer Polytechnic Institute   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIFUNCTIONAL; CHIMERIC ENZYMES; DE NOVO SYNTHESIS; DISACCHARIDE UNITS; DONOR BINDING; DONOR MOLECULES; ENZYME SPECIFICITY; GLYCOSAMINOGLYCANS; GLYCOSYL TRANSFERASE; GLYCOSYLTRANSFERASES; IN-VITRO; KINETIC PROPERTIES; MONODISPERSE; NOVEL CATALYSTS; PASTEURELLA MULTOCIDA; POLYPEPTIDE CHAIN; STRUCTURE/FUNCTION RELATIONSHIPS; SUBSTITUTION PATTERNS; SYNTHASES; UDP-GLUCURONIC ACID; WILD-TYPE ENZYMES;

ISOENZYMES;

SUGARS;

BACTERIAL ENZYME; CARBOHYDRATE DERIVATIVE; GLYCOSYLTRANSFERASE; HEPAROSAN SYNTHASE; HEPAROSAN SYNTHASE 1; HEPAROSAN SYNTHASE 2; ISOENZYME; POLYSACCHARIDE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME BINDING; ENZYME ENGINEERING; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME SYNTHESIS; IN VITRO STUDY; ISOENZYME ANALYSIS; NONHUMAN; PASTEURELLA MULTOCIDA; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA TRANSLATION; STRUCTURE ACTIVITY RELATION; SUBSTITUTION REACTION; WILD TYPE;

BACTERIAL PROTEINS; GLYCOSYLTRANSFERASES; ISOENZYMES; PASTEURELLA MULTOCIDA; POLYSACCHARIDES, BACTERIAL; RECOMBINANT FUSION PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; URIDINE DIPHOSPHATE GLUCURONIC ACID; URIDINE DIPHOSPHATE N-ACETYLGLUCOSAMINE;

PASTEURELLA; PASTEURELLA MULTOCIDA;

EID: 84857710124     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M111.311704     Document Type: Article

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