Effect of glucose feeding on the glycosylation quality of antibody produced by a human cell line, F2N78, in fed-batch culture

Applied Microbiology and Biotechnology

Volumn 98, Issue 8, 2014, Pages 3509-3515

  Seo, Jin Seok ^b   Min, Byung Sup ^a   Kim, Yeon Jung ^a   Cho, Jong Moon ^a   Baek, Eric ^b   Cho, Myung Sam ^a   Lee, Gyun Min ^b  

^a Celltrion Inc   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Aglycosylation; Antibody; F2N78; Glucose feeding; Human cell line; N linked glycosylation

Indexed keywords

BATCH CELL CULTURE; ENZYMES; ESTERIFICATION; FEEDING; GLUCOSE; GLYCOSYLATION; NUTRIENTS;

AGLYCOSYLATION; F2N78; GLUCOSE FEEDING; HUMAN CELL LINES; N-LINKED GLYCOSYLATION;

ANTIBODIES;

IMMUNOGLOBULIN G; ANTIBODY; CULTURE MEDIUM; GLUCOSE; RECOMBINANT PROTEIN;

ANTIBODY PRODUCTION; ARTICLE; BACTERIUM CULTURE; BIOREACTOR; DEPLETION; ELECTROSPRAY; FAST PROTEIN LIQUID CHROMATOGRAPHY; FED BATCH CULTURE; FEEDING; GEL PERMEATION CHROMATOGRAPHY; GLUCOSE DEPLETION; GLUCOSE INTAKE; GLYCOSYLATION; HUMAN; HUMAN CELL; PRECURSOR; PROTEIN EXPRESSION; WESTERN BLOTTING; BATCH CELL CULTURE; CELL LINE; CHEMISTRY; CULTURE MEDIUM; GENETICS; METABOLISM;

ANTIBODIES; BATCH CELL CULTURE TECHNIQUES; CELL LINE; CULTURE MEDIA; GLUCOSE; GLYCOSYLATION; HUMANS; RECOMBINANT PROTEINS;

EID: 84898861924     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-5462-0     Document Type: Article

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