Droplet evaporation method as a new potential approach for highlighting the effectiveness of ultra high dilutions

Complementary Therapies in Medicine

Volumn 22, Issue 2, 2014, Pages 333-340

  Kokornaczyk, Maria Olga ^a   Trebbi, Grazia ^a   Dinelli, Giovanni ^a   Marotti, Ilaria ^a   Bregola, Valeria ^a   Nani, Daniele ^b   Borghini, Francesco ^c   Betti, Lucietta ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b ASL 2   (Italy)

^c UNIVERSITY OF CHIETI   (Italy)

Author keywords

Arsenic trioxide; Droplet evaporation method; Fluctuating asymmetry; Fractal dimension; Polycrystalline structures; Ultra high dilutions; Wheat seedling growth

Indexed keywords

ARSENIC TRIOXIDE; CRYSTALLIN; WATER; ORGANOARSENIC DERIVATIVE; OXIDE;

ARTICLE; CHEMICAL PROCEDURES; CHEMICAL STRESS; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; DILUTION; DROPLET EVAPORATION METHOD; GROWTH RATE; IN VITRO STUDY; NONHUMAN; PLANT GROWTH; PLANT SEED; PLANT STRESS; SHOOT; TRITICUM AESTIVUM; ULTRA HIGH DILUTION; CHEMICAL ANALYSIS; CHEMISTRY; CRYSTALLIZATION; DRUG EFFECTS; GERMINATION; MICROSCOPY; PROCEDURES; WHEAT;

ARSENICALS; CHEMISTRY TECHNIQUES, ANALYTICAL; CRYSTALLIZATION; GERMINATION; MICROSCOPY; OXIDES; SEEDS; TRITICUM; WATER;

EID: 84898774723     PISSN: 09652299     EISSN: 18736963     Source Type: Journal    
DOI: 10.1016/j.ctim.2014.02.005     Document Type: Article

References (56)

1. Deegan R. Pattern formation in drying drops. Physical Review E 2000, 61:475-485.
2. Schena M., Shalon D., Heller R., Chai A., Brown P.O., Davis R.W. Parallel human genome analysis: microarray-based expression monitoring of 1000 genes. Proceedings of the National Academy of Sciences of the United States of America 1996, 93:10614-10619.
3. Jing J., Reed J., Huang J., et al. Automated high resolution optical mapping using arrayed, fluid-fixed DNA molecules. Proceedings of the National Academy of Sciences of the United States of America 1998, 95:8046-8051.
4. Dugas V., Broutin J., Souteyrand E. Droplet evaporation study applied to DNA chip manufacturing. Langmuir 2005, 21:9130-9136.
5. Deng Y., Zhu X.Y., Kienlen T., Guo A. Transport at the air/water interface is the reason for rings in protein microarrays. Journal of American Chemical Society 2006, 128:2768-2769.
6. Arrabito G., Pignataro B. Inkjet printing methodologies for drug screening. Analytical Chemistry 2010, 82:3104-3107.
7. Takhistov P., Chang H.C. Complex stain morphologies. Industrial & Engineering Chemistry Research 2002, 41:6256-6269.
8. Small W.R., Walton C.D., Loos J., Panhuis M. Carbon nanotube network formation from evaporating sessile drops. The Journal of Physical Chemistry B 2006, 110:13029-13036.
9. Kawase T., Sirringhaus H., Friend R.H., Shimoda T. Inkjet printed via-hole interconnections and resistors for all-polymer transistor circuits. Advanced Materials 2001, 13:1601-1605.
10. Chakrapani N., Wei B., Carrillo A., Ajayan P.M., Kane R.S. Capillarity-driven assembly of twodimensional cellular carbon nanotube foams. Proceedings of the National Academy of Sciences of the United States of America 2004, 101:4009-4012.
11. Rapis E. A change in the physical state of a nonequilibrium blood plasma protein film in patients with carcinoma. Technical Physics 2002, 47:510-512.
12. Yakhno Y. Protein phase instability development in plasma of sick patients: clinical observations and model experiments. Natural Science 2002, 2:220-227.
13. Yakhno T.A., Sedova O.A., Sanin A.G., Pelyushenko A.S. On the existence of regular structures in liquid human blood serum (plasma) and phase transitions in the course of its drying. Technical Physics 2003, 48:399-403.
14. Denisov A.B. Algorithm for evaluation of crystal figures obtained after drying of mixed saliva. Bulletin of Experimental Biology and Medicine 2004, 138:30-33.
15. Killeen A.A., Ossina N., McGlennen R.C., et al. Protein self-organization patterns in dried serum reveal changes in B-cell disorders. Molecular Diagnosis & Therapy 2006, 10:371-380.
16. Martusevich A.K., Zimin Y., Bochkareva A. Morphology of dried blood serum specimens of viral hepatitis. Hepatitis 2007, 7(Monthly):207-210.
17. Martusevich A.K., Kamakin N.F. Crystallography of biological fluid as a method for evaluating its physicochemical characteristics. Bulletin of Experimental Biology and Medicine 2007, 143:385-388.
18. Sefiane K. On the formation of regular patterns from drying droplets and their potential use for bio-medical applications. Journal of Bionic Engineering 2010, 7:S82-S93.
19. Kokornaczyk M.O., Dinelli G., Marotti I., Benedettelli S., Nani D., Betti L. Self-organized crystallization patterns from evaporating droplets of common wheat grain leakages as a potential tool for quality analysis. The Scientific World Journal 2011, 11:1712-1725.
20. Deegan R.D., Bakajin O., Dupont T.F., Huber G., Nagel S.R., Witten T.A. Contact line deposits in an evaporating drop. Physical Review E 2000, 62:756-765.
21. Hu H., Larson R.G. Marangoni effect reverses coffee-ring depositions. The Journal of Physical Chemistry B 2006, 110:7090-7094.
22. Bhardwaj R., Fang X., Somasundaran P., Attinger D. Self-assembly of colloidal particles from evaporating droplets: role of DLVO interactions and proposition of a phase diagram. Langmuir 2010, 26:7833-7842.
23. Yakhno T., Yakhno V., Sanin A., Sanina O., Pelyushenko A. Dynamics of phase transitions in drying drops as an information parameter of liquid structure. Nonlinear Dynamics 2005, 39:369-374.
24. Chen G., Mohamed G.J. Complex protein patterns formation via salt-induced self-assembly and droplet evaporation. European Physical Journal E 2010, 33:19-26.
25. Kokornaczyk M.O., Dinelli G., Betti L. Approximate bilateral symmetry in evaporation-induced polycrystalline structures from wheat grain leakages and fluctuating asymmetry as quality indicator. Naturwissenschaften 2013, 100:111-115.
26. Betti L., Trebbi G., Majewsky V., et al. Use of homeopathic preparations in phytopathological models and in field trials: a critical review. Homeopathy 2009, 98:244-266.
27. Marschollek B., Nelle M., Wolf M., Baumgartner S., Heusser P., Wolf U. Effects of exposure to physical factors on homeopathic preparations as determined by ultraviolet light spectroscopy. The Scientific World Journal 2010, 10:49-61.
28. Belon P., Cumps J., Ennis M., et al. Histamine dilutions modulate basophil activation. Inflammation Research 2004, 53:181-188.
29. Frenkel M., Mishra B.M., Sen S., et al. Cytotoxic effects of ultra-diluted remedies on breast cancer cells. International Journal of Oncology 2010, 36:395-403.
30. Endler P.C., Matzer W., Reich C., et al. Seasonal variation of the effect of extremely diluted agitated gibberellic acid (10e-30) on wheat stalk growth: a multi researcher study. The Scientific World Journal 2011, 11:1167-1778.
31. Magnani P., Confori A., Zanoli E., Marzotto M., Bellavite P. Dose-effect study of Gelsemium sempervirens in high dilutions on anxiety-related responses in mice. Psychopharmacology (Berl.) 2010, 210:533-545.
32. Wyss E., Tamm L., Siebenwirth J., Baumgartner S. Homeopathic preparations to control the rosy apple aphid (Dysaphis plantaginea Pass.). The Scientific World Journal 2010, 10:38-48.
33. Khuda-Bukhsh A.R. Towards understanding molecular mechanisms of action of homeopathic drugs: an overview. Molecular and Cellular Biochemistry 2003, 253:339-345.
34. Khuda-Bukhsh A.R., Bhattacharryya S.S., Paul S., Datta S., Boujedaini N., Belon P. Modulation of signal proteins: a plausible mechanism to explain how a potentized drug Secale cor 30C diluted beyond Avogadro's limit combats skin papilloma in mice. Evidence-Based Complementary and Alternative Medicine 2011, 12. Article ID 286320. 10.1093/ecam/nep084.
35. Das D., De A., Dut S., Biswas R., Boujedaini N., Khuda-Bukhsh A.R. Potentized homeopathic drug Arsenicum album 30C positively modulates protein biomarkers and gene expressions in Saccharomyces cerevisiae exposed to arsenate. Journal of Chinese Integrative Medicine 2011, 9:752-760.
36. Saha S.K., Das S., Khuda-Bukhsh A.R. Phenotypic evidence of ultra-highly diluted homeopathic remedies acting at gene expression level: a novel probe on experimental phage infectivity in bacteria. Journal of Chinese Integrative Medicine 2012, 10:462-470.
37. Yinnon T.A., Elia V. Dynamics in perturbed very diluted aqueous solutions: theory and experimental evidence. International Journal of Modern Physics B 2013, 10.1142/s0217979213500057.
38. Betti L., Brizzi M., Nani D., Peruzzi M. Effect of high dilutions of Arsenicum album on wheat seedlings from seeds poisoned with the same substance. British Homeopathic Journal 1997, 86:86-89.
39. Betti L., Elia V., Napoli E., et al. Biological effects and physico-chemical properties of extremely diluted aqueous solutions as a function of aging-time. Frontiers in Life Science 2012, 10.1080/21553769.2011.638986.
40. Brizzi M., Nani D., Peruzzi M., Betti L. Statistical analysis of the effect of high dilutions of arsenic in a large data set from a wheat germination model. British Homeopathic Journal 2000, 89:63-67.
41. 3 high dilution effects on the rate and ariability of wheat seedling growth. Forschende Komplementärmedizin und klassische Naturheilkunde 2005, 12:227-283.
42. Roth C. Literature review and critical analysis on the topic of "In- and Detoxification Experiments in Homeopathy". Berlin Journal of Research in Homeopathy 1991, 1:111-117.
43. Hribar-Marko S., Graunke H., Scherer-Pongratz W., Lothaller H., Endler P.C. Prestimulation of wheat seedlings with gibberellic acid followed by application of an agitated high dilution of the same hormone. International Journal of High Dilution Research 2013, 12:26-39.
44. Baumgartner S., Doesburg P., Scherr C., Andersen J.O. Development of a biocrystallisation assay for examining effects of homeopathic preparations using cress seedlings. Evidence-Based Complementary and Alternative Medicine 2012, 14. Article ID 125945. 10.1155/2012/125945.
45. Kristalle PE, eds. Weises Buchhandlung: Dresden, Germany, 1930.
46. Kahl J., Busscher N., Doesburg P., Mergardt G., Huber M., Ploeger A. First tests of standardized biocrystallization on milk and milk products. European Food Research and Technology 2009, 229:175-178.
47. Busscher N., Kahl J., Andersen J.O., et al. Standardization of the biocrystallization method for carrot samples. Biological Agriculture and Horticulture 2010, 27:1-23.
48. Stock-Schröer B., Albrecht H., Betti L., et al. Reporting experiments in homeopathic basic research (REHBaR) - a detailed guideline for authors. Homeopathy 2009, 98(4):287-298.
49. Brizzi M., Elia V., Trebbi G., Nani D., Peruzzi M., Betti L. The effectiveness of ultramolecular aqueous dilutions on a wheat germination model as a function of heat and aging-time. Evidence-Based Complementary and Alternative Medicine 2011, 10.1093/ecam/nep217.
50. Collins T.J. ImageJ for microscopy. Biotechniques 2007, 43:25-30.
51. Karperien. FracLac for ImageJ, version 2.5. http://rsb.info.nih.gov/ij/plugins/fraclac/FLHelp./Introduction.htm.
52. Lamari L. Assess: image analysis software for plant disease quantification 2002, APS Press, St. Paul, Minnesota, USA.
53. Witt C.M., Lüdtke R., Weisshuhn T.E., Quint P., Willich S.N. The role of trace elements in homeopathic preparations and the influence of container material, storage duration, and potentisation. Forschende Komplementärmedizin 2006, 13(1):15-21.
54. Baumgartner S., Heusser P., Thurneysen A. Methodological standards and problems in preclinical homeopathic potency research. Forschende Komplementärmedizin 1998, 5:27-32.
55. Bellavite P., Marzotto M., Olioso D., Moratti E., Conforti A. High-dilution effects revisited. 1. Physicochemical aspects. Homeopathy 2014, 103(1):4-21.
56. Betti L., Trebbi G., Kokornaczyk M.O., Nani D., Peruzzi M., Brizzi M. Effectiveness of ultra high diluted arsenic is a function of succussion number as evidenced by wheat germination test and droplet evaporation method. International Journal of High Dilution Research 2013, 12(44):127-128. Proceedings of the XXVII GIRI symposium. Switzerland: Bern; September 3-4, 2013.