Phenotypic evidence of ultra-highly diluted homeopathic remedies acting at gene expression level: A novel probe on experimental phage infectivity in bacteria

Journal of Chinese Integrative Medicine

Volumn 10, Issue 4, 2012, Pages 462-470

  Saha, Santu Kumar ^a   Das, Sreemanti ^a   Khuda Bukhsh, Anisur Rahman ^a  

^a UNIVERSITY OF KALYANI   (India)

Author keywords

Bacteriophage phi X 174; Escherichia coli; Gene expression regulation; Homeopathy; Plaque assay

Indexed keywords

AGAR; ARNICA MONTANA EXTRACT; BACTERIAL DNA; HOMEOPATHIC AGENT;

ANTIVIRAL ACTIVITY; ARNICA MONTANA; ARTICLE; ATROPA BELLADONNA; BACTERIOPHAGE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE CLUSTER; GENE EXPRESSION; GENE PRODUCT; HOMEOPATHY; NONHUMAN; PHENOTYPE; POISON IVY; VIRUS INFECTIVITY;

BACTERIOPHAGE PHI X 174; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; MATERIA MEDICA; PHENOTYPE;

EID: 84859777970     PISSN: 16721977     EISSN: None     Source Type: Journal    
DOI: 10.3736/jcim20120416     Document Type: Article

References (36)

1. Sinsheimer RL, Starman B, Nagler C, Guthrie S. The process of infection with bacteriophage ΦX174. I. Evidence for a replicative form. J Mol Biol. 1962: 142-160.
2. Arai K, Low R, Kobori J, Shlomai J, Kornberg A. Mechanism of dnaB protein action. V. Association of dnaB protein, protein n', and other repriming proteins in the primosome of DNA replication. J Biol Chem. 1981; 256(10): 5273-5280.
3. Goulian M, Kornberg A, Sinsheimer RL. Enzymatic synthesis of DNA, XTN • Synthesis of infectious phage Φ-X174 DNA. Proc Natl Acad Sei USA. 1967; 58 (6): 2321-2328.
4. Kornberg A. Ten commandments: lessons from the enzymology of DNA replication. J Bacteriol. 2000; 182 (13): 3613-3618.
5. Sanger F, Coulson AR, Friedmann T, Air GM, Barrell BG, Brown NL, Fiddes JC, Hutchison CA 3rd, Slocombe PM, Smith M. The nucleotide sequence of bacteriophage ΦX174. J Mol Biol. 1978; 125(2): 225-246.
6. Smith HO, Hutchison CA 3rd, Pfannkoch C, Venter JC. Generating a synthetic genome by whole genome assembly: (ΦX174 bacteriophage from synthetic oligonu-cleotides. Proc Natl Acad Sei USA. 2003; 100(26): 15440-15445.
7. Witte A, Lubitz W. Biochemical characterization of (ΦX174-protein-E-mediated lysis of Escherichia coli. Eur J Biochem. 1989; 180(2): 393-398.
8. Eigner J, Stouthamer AH, van der Sluys I, Cohen JA. A study of the 70 s component of bacteriophage ΦX174. J Mol Biol. 1963; 6(1): 61-84.
9. Markert A, Zillig W. Studies on the lysis of Escherichia coli C by bacteriophage ΦX174. Virology. 1965; 25: 88-97.
10. Cao Y, Lu HM, Liang J. Stochastic probability landscape model for switching efficiency, robustness, and differential threshold for induction of genetic circuit in phage X. Conf Proc IEEE Eng Med Biol Soc. 2008; 2008: 611-614.
11. Khan NC, Poddar RK. Photodynamic inactivation of antigenic determinants of single-stranded DNA bacteriophage ΦX174. J Virol. 1974; 13(5): 997-1000.
12. de Mik G, de Groot I. Mechanisms of inactivation of bacteriophage (ΦX174 and its DNA in aerosols by ozone and ozonized cyclohexene. J Hyg (Lond). 1977; 78 (2): 199-211.
13. Hsu WT, Lin EJ, Harvey RG, Weiss SB. Mechanism of phage (ΦX174 DNA inactivation by benzo(a)pyrene-7, 8-dihydrodiol-9,10-epoxide. Proc Natl Acad Sei USA. 1977; 74(8): 3335-3339.
14. Rueckert RR, Zillig W. Biosynthesis of virus protein in Escherichia coli C in vivo following infection with bacteriophage ΦX174. J Mol Biol. 1962; 5: 1-9.
15. Hayashi M, Hayashi MN, Spiegelman S. Restriction of in vivo genetic transcription to one of the complementary strands of DNA. Proc Natl Acad Sei USA. 1963; 50: 664-672.
16. Khuda-Bukhsh AR. Towards understanding molecular mechanisms of action of homeopathic drugs: an overview. Mol Cell Biochem. 2003; 253(1-2): 339-345.
17. Dulbecco R. Experiments on photoreactivation of bacte-riophages inactivated with ultraviolet radiation. J Bacteriol. 1950; 59(3): 329-347.
18. Denhardt DT, Sinsheimer RL. The process of infection with bacteriophage ΦX174. 3. Phage maturation and lysis after synchronized infection. J Mol Biol. 1965; 12 (3): 641-646.
19. Saha S, Jana B, Basu T. The two inducible responses, SOS and heat-shock, in Escherichia coli act synergistically during Weigle reactivation of the bacteriophage ΦX174. Int J Radiat Biol. 2007; 83(7): 463-469.
20. Goossens M, Laekeman G, Aertgeerts B, Buntinx F; ARCH study group. Evaluation of the quality of life after individualized homeopathic treatment for seasonal allergic rhinitis. A prospective, open, non-comparative study. Homeopathy. 2009; 98(1): 11-16.
21. Kirkby R, Herscu P. Homeopathic trial design in influenza treatment. Homeopathy. 2010; 99(1): 69-75.
22. Lockie A. The family guide to homeopathy: the safe form of medicine for the future. London: Elm Tree Books. 1989.
23. Horvath P, Barrangou R. CRISPR/Cas, the immune system of bacteria and archaea. Science. 2010; 327 (5962): 167-170.
24. Makarova KS, Wolf YI, Snir S, Koonin EV. Defense islands in bacterial and archaeal genomes and prediction of novel defense systems. J Bacteriol. 2011; 193(21): 6039-6056.
25. Khuda-Bukhsh AR, De A, Das D, Dutta S, Boujedaini N. Analysis of the capability of ultra-highly diluted glucose to increase glucose uptake in arsenite-stressed bacteria Escherichia coli. J Chin Integr Med. 2011; 9 (8): 901-912. English with abstract in Chinese.
26. De A, Das D, Dutta S, Chakraborty D, Boujedaini N, Khuda-Bukhsh AR. Potentized homeopathic drug Arsenicum Album 30C inhibits intracellular reactive oxygen species generation and up-regulates expression of arsenic resistance gene in arsenic-exposed bacteria Escherichia coli. J Chin Integr Med. 2012; 10(2): 210-227. English with abstract in Chinese.
27. Das S, Saha SK, De A, Das D, Khuda-Bukhsh AR. A study of the potential of the homeopathic remedy, Arnica Montana 30C, to reduce DNA damage in Escherichia coli exposed to ultraviolet irradiation through up-regulation of nucleotide excision repair genes. J Chin Integr Med. 2012; 10(3): 337-346. English with abstract in Chinese.
28. Maratea D, Young K, Young R. Deletion and fusion analysis of the phage (ΦX174 lysis gene E. Gene. 1985; 40(1): 39-46.
29. Kawakami AP, Osugui L, Cesar AT, Priven SW, de Carvalho VM, Bonamin LV. In vitro growth of uropathogenic Escherichia coli isolated from a snow leopard treated with homeopathic and isopathic remedies: a pilot study. IntJ High Dilution Res. 2009; 8(27): 41-44.
30. Khuda-Bukhsh AR. Potentized homeopathic drugs act through regulation of gene-expression: a hypothesis to explain their mechanism and pathways of action in vivo. Complement Ther Med. 1997; 5(1): 43-46.
31. Khuda-Bukhsh AR. Laboratory research in homeopathy: pro. Integr Cancer Ther. 2006; 5(4): 320-332.
32. Khuda-Bukhsh AR. Mice as a model for homeopathy research. Homeopathy. 2009; 98(4): 267-279.
33. Khuda-Bukhsh AR, Pathak S. Homeopathic drug discovery: theory update and methodological aspect. Expert Opin Drug Discov. 2008; 3(8): 979-990.
34. Mallick P, Mallick JC, Guha B, Khuda-Bukhsh AR. Ameliorating effect of microdoses of a potentized homeopathic drug, Arsenicum Album, on arsenic-induced toxicity in mice. BMC Complement Altern Med. 2003; 3: 7.
35. Khuda-Bukhsh AR, Bhattacharyya SS, Paul S, Dutta S, Boujedaini N, Belon P. Modulation of signal proteins: a plausible mechanism to explain how a potentized drug Secale Cor 30C diluted beyond Avogadro's limit combats skin papilloma in mice. Evid Based Complement Alternat Med. 2011: 286320.
36. Das D, De A, Dutta S, Biswas R, Boujedaini N, Khuda-Bukhsh AR. Potentized homeopathic drug Arsenicum Album 30C positively modulates protein biomarkers and gene expressions in Saccharomyces cerevisae exposed to arsenate. J Chin Integr Med. 2011; 9(7): 752-760. English with abstract in Chinese