Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers by droplet digital PCR

Human Gene Therapy Methods

Volumn 25, Issue 2, 2014, Pages 115-125

  Lock, Martin ^a   Alvira, Mauricio R ^a   Chen, Shu Jen ^a   Wilson, James M ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AGAROSE; ENHANCED GREEN FLUORESCENT PROTEIN; GENOMIC DNA; GROWTH HORMONE; PARVOVIRUS VECTOR; PLASMID DNA; PROTEINASE K; THYROXINE BINDING GLOBULIN; PRIMER DNA; SINGLE STRANDED DNA;

ADENO ASSOCIATED VIRUS; ARTICLE; CONTROLLED STUDY; CYTOMEGALOVIRUS; DROPLET DIGITAL POLYMERASE CHAIN REACTION; ELECTROPHORESIS; GENOME SIZE; GENOMIC FRAGMENT; NONHUMAN; POLYADENYLATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROMOTER REGION; REPRODUCIBILITY; SIMIAN VIRUS 40; SOUTHERN BLOTTING; TITRIMETRY; VIRUS CAPSID; VIRUS GENOME; AGAR GEL ELECTROPHORESIS; ANALYSIS; DEPENDOPARVOVIRUS; GENE VECTOR; GENETICS; HEK293 CELL LINE; HUMAN; METABOLISM;

DEPENDOVIRUS; DNA PRIMERS; DNA, SINGLE-STRANDED; ELECTROPHORESIS, AGAR GEL; GENETIC VECTORS; GENOME, VIRAL; HEK293 CELLS; HUMANS; POLYMERASE CHAIN REACTION;

EID: 84898759293     PISSN: 19466536     EISSN: 19466544     Source Type: Journal    
DOI: 10.1089/hgtb.2013.131     Document Type: Article

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