Oxidative phosphorylation measurement in cell lines and tissues

Mitochondrion

Volumn 15, Issue 1, 2014, Pages 24-33

  Van Bergen, Nicole J ^a,b,c   Blake, Rachel E ^a,b,c   Crowston, Jonathan G ^a,b,c   Trounce, Ian A ^a,b,c,d  

^a CENTRE FOR EYE RESEARCH AUSTRALIA   (Australia)

^b UNIVERSITY OF MELBOURNE   (Australia)

^c ROYAL VICTORIAN EYE AND EAR HOSPITAL   (Australia)

^d ST VINCENT S HOSPITAL   (Australia)

Author keywords

ATP synthesis; Enzymology; Mitochondria; Oxidative phosphorylation; Protocol; Respiration

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; CITRATE SYNTHASE; CITRININ; CYTOCHROME C OXIDASE; DIGITONIN; UBIQUINOL CYTOCHROME C REDUCTASE;

ANIMAL TISSUE; ARTICLE; B LYMPHOCYTE; BINDING AFFINITY; BRAIN MITOCHONDRION; CELL CULTURE; CELL FUNCTION; CELL GROWTH; CELL ISOLATION; CELL LINE; CELL PERMEABILIZATION; CELL RESPIRATION; CELL TRANSFORMATION; CONTROLLED STUDY; DNA MODIFICATION; ENZYME ACTIVITY; ENZYME BINDING; ENZYME KINETICS; ENZYME SUBSTRATE; ENZYMOLOGY; EPSTEIN BARR VIRUS; HUMAN; HUMAN CELL; MITOCHONDRIAL FUNCTION; MITOCHONDRIAL ISOLATION; MITOCHONDRION; MOUSE; NONHUMAN; OXIDATIVE PHOSPHORYLATION; PERIPHERAL LYMPHOCYTE; PRIORITY JOURNAL; PROTEIN SYNTHESIS; TISSUE CULTURE; TISSUES; WESTERN BLOTTING; CYTOLOGY; METABOLISM; PROCEDURES;

CELL LINE; CYTOLOGICAL TECHNIQUES; HUMANS; MITOCHONDRIA; OXIDATIVE PHOSPHORYLATION;

EID: 84897930960     PISSN: 15677249     EISSN: 18728278     Source Type: Journal    
DOI: 10.1016/j.mito.2014.03.003     Document Type: Article

References (52)

1. Abramoff M.D., Magelhaes P.J., Ram S.J. Image processing with ImageJ. Biophotonics Int. 2004, 11:36-42.
2. Acehan D., Xu Y., Stokes D.L., Schlame M. Comparison of lymphoblast mitochondria from normal subjects and patients with Barth syndrome using electron microscopic tomography. Lab. Investig. J. Tech. Methods Pathol. 2007, 87:40-48.
3. Anderson M.F., Sims N.R. Improved recovery of highly enriched mitochondrial fractions from small brain tissue samples. Brain Res Brain Res Protoc 2000, 5:95-101.
4. Barrientos A. In vivo and in organello assessment of OXPHOS activities. Methods 2002, 26:307-316.
5. Barrientos A., Fontanesi F., Diaz F. Evaluation of the mitochondrial respiratory chain and oxidative phosphorylation system using polarography and spectrophotometric enzyme assays. Methods 2002 Apr, 26(4):307-316.
6. Bonora E., Porcelli A.M., Gasparre G., Biondi A., Ghelli A., Carelli V., Baracca A., Tallini G., Martinuzzi A., Lenaz G., Rugolo M., Romeo G. Defective oxidative phosphorylation in thyroid oncocytic carcinoma is associated with pathogenic mitochondrial DNA mutations affecting complexes I and III. Cancer Res. 2006, 66:6087-6096.
7. Brown M., Trounce I., Jun A., Allen J., Wallace D. Functional analysis of lymphoblast and cybrid mitochondria containing the 11778, 3460 or 14484 Leber's hereditary optic neuropathy mitochondrial DNA mutation. J. Biol. Chem. 2000, 275:39831-39836.
8. Chaturvedi R.K., Flint Beal M. Mitochondrial diseases of the brain. Free Radic. Biol. Med. 2013, 63:1-29.
9. Chretien D., Gallego J., Barrientos A., Casademont J., Cardellach F., Munnich A., Rotig A., Rustin P. Biochemical parameters for the diagnosis of mitochondrial respiratory chain deficiency in humans, and their lack of age-related changes. Biochem. J. 1998, 329(Pt 2):249-254.
10. Clark J.B., Nicklas W.J. The metabolism of rat brain mitochondria. Preparation and characterization. J. Biol. Chem. 1970, 245:4724-4731.
11. Estornell E., Fato R., Pallotti F., Lenaz G. Assay conditions for the mitochondrial NADH:coenzyme Q oxidoreductase. FEBS Lett. 1993, 332:127-131.
12. Frazier A.E., Thorburn D.R. Biochemical analyses of the electron transport chain complexes by spectrophotometry. Methods Mol. Biol. 2012, 837:49-62.
13. Gellerich F.N., Mayr J.A., Reuter S., Sperl W., Zierz S. The problem of interlab variation in methods for mitochondrial disease diagnosis: enzymatic measurement of respiratory chain complexes. Mitochondrion 2004, 4:427-439.
14. Gerencser A.A., Neilson A., Choi S.W., Edman U., Yadava N., Oh R.J., Ferrick D.A., Nicholls D.G., Brand M.D. Quantitative microplate-based respirometry with correction for oxygen diffusion. Anal. Chem. 2009, 81:6868-6878.
15. Graham J.M. Isolation of mitochondria from tissues and cells by differential centrifugation. Current Protocols in Cell Biology 1999, vol. 3.3. John Wiley & Sons, Inc. J.S. Bonifacino, M. Dasso, J.B. Harford, J. Lippincott-Schwartz, K.M. Yamada (Eds.).
16. Holtzman E. Lysosomes 1989, Plenum Press, New York.
17. Hussain T., Kotnis A., Sarin R., Mulherkar R. Establishment & characterization of lymphoblastoid cell lines from patients with multiple primary neoplasms in the upper aero-digestive tract & healthy individuals. Indian J. Med. Res. 2012, 135:820-829.
18. Hutter E., Renner K., Pfister G., Stockl P., Jansen-Durr P., Gnaiger E. Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem. J. 2004, 380:919-928.
19. Hutter E., Unterluggauer H., Garedew A., Jansen-Durr P., Gnaiger E. High-resolution respirometry-a modern tool in aging research. Exp. Gerontol. 2006, 41:103-109.
20. Invernizzi F., D'Amato I., Jensen P.B., Ravaglia S., Zeviani M., Tiranti V. Microscale oxygraphy reveals OXPHOS impairment in MRC mutant cells. Mitochondrion 2012, 12:328-335.
21. Kang C.H., Ferguson-Miller S., Margoliash E. Steady state kinetics and binding of eukaryotic cytochromes c with yeast cytochrome c peroxidase. J. Biol. Chem. 1977, 252:919-926.
22. Kirby D.M., Thorburn D.R. Approaches to finding the molecular basis of mitochondrial oxidative phosphorylation disorders. Twin Res. Hum. Genet. 2008, 11:395-411.
23. Lai J.C., Clark J.B. Preparation of synaptic and nonsynaptic mitochondria from mammalian brain. Methods Enzymol. 1979, 55:51-60.
24. Lee S., Sheck L., Crowston J.G., Van Bergen N.J., O'Neill E.C., O'Hare F., Kong Y.X., Chrysostomou V., Vincent A.L., Trounce I.A. Impaired complex-I-linked respiration and ATP synthesis in primary open-angle glaucoma patient lymphoblasts. Invest. Ophthalmol. Vis. Sci. 2012, 53:2431-2437.
25. Manfredi G., Yang L., Gajewski C.D., Mattiazzi M. Measurements of ATP in mammalian cells. Methods 2002, 26:317-326.
26. Mayorov V.I., Lowrey A.J., Biousse V., Newman N.J., Cline S.D., Brown M.D. Mitochondrial oxidative phosphorylation in autosomal dominant optic atrophy. BMC Biochem. 2008, 9:22.
27. Medja F., Allouche S., Frachon P., Jardel C., Malgat M., de Camaret B.M., Slama A., Lunardi J., Mazat J.P., Lombes A. Development and implementation of standardized respiratory chain spectrophotometric assays for clinical diagnosis. Mitochondrion 2009, 9:331-339.
28. Miro O., Cardellach F., Barrientos A., Casademont J., Rotig A., Rustin P. Cytochrome c oxidase assay in minute amounts of human skeletal muscle using single wavelength spectrophotometers. J. Neurosci. Methods 1998, 80:107-111.
29. Moreadith R., Fiskum G. Isolation of mitochondria from ascites tumor cells permeabilized with digitonin. Anal. Biochem. 1984, 137:360-367.
30. Pecinova A., Drahota Z., Nuskova H., Pecina P., Houstek J. Evaluation of basic mitochondrial functions using rat tissue homogenates. Mitochondrion 2011, 11:722-728.
31. Pesta D., Gnaiger E. High-resolution respirometry: OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol. Biol. 2012, 810:25-58.
32. Scatena R. Mitochondria and cancer: a growing role in apoptosis, cancer cell metabolism and dedifferentiation. Adv. Exp. Med. Biol. 2012, 942:287-308.
33. Schoer J.K., Gallegos A.M., McIntosh A.L., Starodub O., Kier A.B., Billheimer J.T., Schroeder F. Lysosomal membrane cholesterol dynamics. Biochemistry 2000, 39:7662-7677.
34. Sherratt H.S., Watmough N.J., Johnson M.A., Turnbull D.M. Methods for study of normal and abnormal skeletal muscle mitochondria. Methods Biochem. Anal. 1988, 33:243-335.
35. Sims N.R. Rapid isolation of metabolically active mitochondria from rat brain and subregions using Percoll density gradient centrifugation. J. Neurochem. 1990, 55:698-707.
36. Speck S.H., Dye D., Margoliash E. Single catalytic site model for the oxidation of ferrocytochrome c by mitochondrial cytochrome c oxidase. Proc. Natl. Acad. Sci. U. S. A. 1984, 81:347-351.
37. Spinazzi M., Casarin A., Pertegato V., Salviati L., Angelini C. Assessment of mitochondrial respiratory chain enzymatic activities on tissues and cultured cells. Nat. Protoc. 2012, 7:1235-1246.
38. 2-mediated oxidative stress versus cold ischemia-reperfusion: mitochondrial respiratory defects in cultured human endothelial cells. Transplantation 2002, 74:1800-1803.
39. Tatuch Y., Robinson B.H. The mitochondrial DNA mutation at 8993 associated with NARP slows the rate of ATP synthesis in isolated lymphoblast mitochondria. Biochem. Biophys. Res. Commun. 1993, 192:124-128.
40. Taylor R.W., Birch-Machin M.A., Bartlett K., Turnbull D.M. Succinate-cytochrome c reductase: assessment of its value in the investigation of defects of the respiratory chain. Biochim. Biophys. Acta 1993, 1181:261-265.
41. Thorburn D.R., Chow C.W., Kirby D.M. Respiratory chain enzyme analysis in muscle and liver. Mitochondrion 2004, 4:363-375.
42. Trounce I., Kim Y., Jun A., Wallace D. Assessment of mitochondrial oxidative phosphorylation in patient muscle biopsies, lymphoblasts, and transmitochondrial cell lines. Methods Enzymol. 1996, 264:484-509.
43. Van Bergen N.J., Crowston J.G., Kearns L.S., Staffieri S.E., Hewitt A.W., Cohn A.C., Mackey D.A., Trounce I.A. Mitochondrial oxidative phosphorylation compensation may preserve vision in patients with OPA1-linked autosomal dominant optic atrophy. PLoS One 2011, 6:e21347.
44. van den Bogert C., Spelbrink J.N., Dekker H.L. Relationship between culture conditions and the dependency on mitochondrial function of mammalian cell proliferation. J. Cell. Physiol. 1992, 152:632-638.
45. Wallace D.C. Mitochondrial genetics: a paradigm for aging and degenerative diseases?. Science 1992, 256:628-632.
46. Wallace D.C. Mitochondrial DNA mutations in disease and aging. Environ. Mol. Mutagen. 2010, 51:440-450.
47. Wallace D.C., Zheng X.X., Lott M.T., Shoffner J.M., Hodge J.A., Kelley R.I., Epstein C.M., Hopkins L.C. Familial mitochondrial encephalomyopathy (MERRF): genetic, pathophysiological, and biochemical characterization of a mitochondrial DNA disease. Cell 1988, 55:601-610.
48. Wu M., Neilson A., Swift A.L., Moran R., Tamagnine J., Parslow D., Armistead S., Lemire K., Orrell J., Teich J., Chomicz S., Ferrick D.A. Multiparameter metabolic analysis reveals a close link between attenuated mitochondrial bioenergetic function and enhanced glycolysis dependency in human tumor cells. Am. J. Physiol. Cell Physiol. 2007, 292:C125-C136.
49. Xu Y., Sutachan J.J., Plesken H., Kelley R.I., Schlame M. Characterization of lymphoblast mitochondria from patients with Barth syndrome. Lab. Invest. 2005, 85:823-830.
50. Ye F., Hoppel C.L. Measuring oxidative phosphorylation in human skin fibroblasts. Anal. Biochem. 2013, 437:52-58.
51. Zanna C., Ghelli A., Porcelli A.M., Martinuzzi A., Carelli V., Rugolo M. Caspase-independent death of Leber's hereditary optic neuropathy cybrids is driven by energetic failure and mediated by AIF and Endonuclease G. Apoptosis 2005, 10:997-1007.
52. Zheng X.X., Shoffner J.M., Voljavec A.S., Wallace D.C. Evaluation of procedures for assaying oxidative phosphorylation enzyme activities in mitochondrial myopathy muscle biopsies. Biochim. Biophys. Acta 1990, 1019:1-10.