Evaluation of basic mitochondrial functions using rat tissue homogenates

Mitochondrion

Volumn 11, Issue 5, 2011, Pages 722-728

  Pecinová, Alena ^a   Drahota, Zdeněk ^a   Nusková, Hana ^a   Pecina, Petr ^a   Houštěk, Josef ^a  

^a INSTITUTE OF MATHEMATICS   (Czech Republic)

Author keywords

Isolated mitochondria; Mitochondrial membrane potential; Oxidative phosphorylation; Respiratory control; Tissue homogenates

Indexed keywords

CITRATE SYNTHASE; CYTOCHROME C OXIDASE;

ANATOMICAL VARIATION; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN; CONTROLLED STUDY; ENZYME ACTIVITY; HEART; KIDNEY; LIVER; MALE; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION; NONHUMAN; OXIDATIVE PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN LOCALIZATION; QUALITATIVE ANALYSIS; RAT; TISSUE HOMOGENATE;

ANIMALS; BRAIN; CITRATE (SI)-SYNTHASE; ELECTRON TRANSPORT CHAIN COMPLEX PROTEINS; ELECTRON TRANSPORT COMPLEX IV; ENZYME ASSAYS; KIDNEY; LIVER; MALE; MEMBRANE POTENTIAL, MITOCHONDRIAL; MITOCHONDRIA; MITOCHONDRIAL PROTEINS; MYOCARDIUM; OXIDATIVE PHOSPHORYLATION; OXYGEN CONSUMPTION; RATS; RATS, WISTAR;

RATTUS;

EID: 79961209116     PISSN: 15677249     EISSN: 18728278     Source Type: Journal    
DOI: 10.1016/j.mito.2011.05.006     Document Type: Article

References (28)

1. Benard G., Faustin B., Passerieux E., Galinier A., Rocher C., Bellance N., Delage J.P., Casteilla L., Letellier T., Rossignol R. Physiological diversity of mitochondrial oxidative phosphorylation. Am. J. Physiol. Cell Physiol. 2006, 291:C1172-C1182.
2. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 1976, 72:248-254.
3. Cannon B., Nedergaard J. Studies of thermogenesis and mitochondrial function in adipose tissues. Methods Mol. Biol. 2008, 456:109-121.
4. Cervinkova Z., Lotkova H., Krivakova P., Rousar T., Kucera O., Tichy L., Cervinka M., Drahota Z. Evaluation of mitochondrial function in isolated rat hepatocytes and mitochondria during oxidative stress. Altern. Lab. Anim. 2007, 35:353-361.
5. Cossarizza A., Ceccarelli D., Masini A. Functional heterogeneity of an isolated mitochondrial population revealed by cytofluorometric analysis at the single organelle level. Exp. Cell Res. 1996, 222:84-94.
6. Fernandez-Vizarra E., Lopez-Perez M.J., Enriquez J.A. Isolation of biogenetically competent mitochondria from mammalian tissues and cultured cells. Methods 2002, 26:292-297.
7. Fernandez-Vizarra E., Ferrin G., Perez-Martos A., Fernandez-Silva P., Zeviani M., Enriquez J.A. Isolation of mitochondria for biogenetical studies: an update. Mitochondrion 2010, 10:253-262.
8. Ferreira R., Vitorino R., Alves R.M., Appell H.J., Powers S.K., Duarte J.A., Amado F. Subsarcolemmal and intermyofibrillar mitochondria proteome differences disclose functional specializations in skeletal muscle. Proteomics 2010, 10:3142-3154.
9. Guo D., Nguyen T., Ogbi M., Tawfik H., Ma G., Yu Q., Caldwell R.W., Johnson J.A. Protein kinase C-epsilon coimmunoprecipitates with cytochrome oxidase subunit IV and is associated with improved cytochrome-c oxidase activity and cardioprotection. Am. J. Physiol. Heart Circ. Physiol. 2007, 293:H2219-H2230.
10. Kondrashova M.N., Fedotcheva N.I., Saakyan I.R., Sirota T.V., Lyamzaev K.G., Kulikova M.V., Temnov A.V. Preservation of native properties of mitochondria in rat liver homogenate. Mitochondrion 2001, 1:249-267.
11. Kondrashova M., Zakharchenko M., Khunderyakova N. Preservation of the in vivo state of mitochondrial network for ex vivo physiological study of mitochondria. Int. J. Biochem. Cell Biol. 2009, 41:2036-2050.
12. Kuznetsov A.V., Strobl D., Ruttmann E., Konigsrainer A., Margreiter R., Gnaiger E. Evaluation of mitochondrial respiratory function in small biopsies of liver. Anal. Biochem. 2002, 305:186-194.
13. Kuznetsov A.V., Schneeberger S., Seiler R., Brandacher G., Mark W., Steurer W., Saks V., Usson Y., Margreiter R., Gnaiger E. Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am. J. Physiol. Heart Circ. Physiol. 2004, 286:H1633-H1641.
14. Labajova A., Vojtiskova A., Krivakova P., Kofranek J., Drahota Z., Houstek J. Evaluation of mitochondrial membrane potential using a computerized device with a tetraphenylphosphonium-selective electrode. Anal. Biochem. 2006, 353:37-42.
15. Mracek T., Pecinova A., Vrbacky M., Drahota Z., Houstek J. High efficiency of ROS production by glycerophosphate dehydrogenase in mammalian mitochondria. Arch. Biochem. Biophys. 2009, 481:30-36.
16. Pallotti F., Lenaz G. Isolation and subfractionation of mitochondria from animal cells and tissue culture lines. Methods Cell Biol. 2007, 80:3-44.
17. Palmer J.W., Tandler B., Hoppel C.L. Biochemical properties of subsarcolemmal and interfibrillar mitochondria isolated from rat cardiac muscle. J. Biol. Chem. 1977, 252:8731-8739.
18. Pecina P., Capkova M., Chowdhury S.K., Drahota Z., Dubot A., Vojtiskova A., Hansikova H., Houst'kova H., Zeman J., Godinot C., Houstek J. Functional alteration of cytochrome c oxidase by SURF1 mutations in Leigh syndrome. Biochim. Biophys. Acta 2003, 1639:53-63.
19. Plasek J., Vojtiskova A., Houstek J. Flow-cytometric monitoring of mitochondrial depolarisation: from fluorescence intensities to millivolts. J. Photochem. Photobiol. B 2005, 78:99-108.
20. Raffaella C., Francesca B., Italia F., Marina P., Giovanna L., Susanna I. Alterations in hepatic mitochondrial compartment in a model of obesity and insulin resistance. Obesity (Silver Spring) 2008, 16:958-964.
21. Saks V.A., Veksler V.I., Kuznetsov A.V., Kay L., Sikk P., Tiivel T., Tranqui L., Olivares J., Winkler K., Wiedemann F., Kunz W.S. Permeabilized cell and skinned fiber techniques in studies of mitochondrial function in vivo. Mol. Cell. Biochem. 1998, 184:81-100.
22. Schagger H., von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100kDa. Anal. Biochem. 1987, 166:368-379.
23. Sperl W., Skladal D., Gnaiger E., Wyss M., Mayr U., Hager J., Gellerich F.N. High resolution respirometry of permeabilized skeletal muscle fibers in the diagnosis of neuromuscular disorders. Mol. Cell. Biochem. 1997, 174:71-78.
24. Srere P.A. Citrate synthase. Methods Enzymol. 1969, 13:3-26.
25. Wharton D.C., Tzagoloff A. Cytochrome oxidase from beef heart mitochondria. Methods Enzymol. 1967, 10:245-253.
26. Wiley S.E., Rardin M.J., Dixon J.E. Chapter 13 localization and function of the 2Fe-2S outer mitochondrial membrane protein mitoNEET. Methods Enzymol. 2009, 456:233-246.
27. Wittig I., Braun H.P., Schagger H. Blue native PAGE. Nat. Protoc. 2006, 1:418-428.
28. Wittig I., Carrozzo R., Santorelli F.M., Schagger H. Functional assays in high-resolution clear native gels to quantify mitochondrial complexes in human biopsies and cell lines. Electrophoresis 2007, 28:3811-3820.