Detection of hemolytic strains of Aeromonas hydrophila and A. Sobria along with other Aeromonas spp. from fish and fishery products by multiplex PCR

Journal of Food Science and Technology

Volumn 51, Issue 2, 2014, Pages 401-407

  Hussain, I A ^a   Jeyasekaran, G ^a   Shakila, R Jeya ^a   Raj, K T ^a   Jeevithan, E ^a  

^a TAMIL NADU DR J JAYALALITHAA FISHERIES UNIVERSITY   (India)

Author keywords

Aeromonas hydrophila; Fish and fishery products; Hemolytic strains; Multiplex PCR; Species specific detection

Indexed keywords

FISHERIES; POLYMERASE CHAIN REACTION; RNA;

AEROMONAS HYDROPHILA; COMMERCIAL FISH; DETECTION LIMITS; FISHERY PRODUCT; MULTIPLEX PCR; RELIABLE RESULTS; SIMULTANEOUS DETECTION; SPECIES SPECIFICS;

FISH;

EID: 84897675912     PISSN: 00221155     EISSN: 09758402     Source Type: Journal    
DOI: 10.1007/s13197-013-1190-9     Document Type: Article

References (44)

1. Aggar WA, McCormick JD, Gurwith MJ (1985) Clinical and microbiological features of Aeromonas hydrophila-associated diarrhea. J Clin Microbiol 21:909-913
2. Balakrishna K, Murali HS, Batra HV (2010) Detection of toxigenic strains of Aeromonas species in food by a multiplex PCR assay. Indian J Microbiol 50:139-144
3. Beaz-Hidalgo R, Alperi A, Bujan N, Romalde JL, Figueras MJ (2010) Comparison of phenotypical and genetic identification of Aeromonas strains isolated from diseased fish. Syst Appl Microbiol 33:149-153
4. Bin Kingombe CI, Huys G, Tonolla M, Albert MJ, Swings J, Peduzzi R, Jemmi T (1999) PCR detection, characterization, and distribution of virulence genes in Aeromonas spp. Appl Environ Microbiol 65:5293-5302
5. Bin Kingombe CI, Huys G, Howald D, Luthia E, Swings J, Jemmia T (2004) The usefulness of molecular techniques to assess the presence of Aeromonas spp. harboring virulence markers in foods. Int J Food Microbiol 94:113-121
6. Bin Kingombe CI, Aoust JYD, Huys G, Hofmann L, Rao M, Kwan J (2010) Multiplex PCR method for detection of three Aeromonas enterotoxin genes. Appl Environ Microbiol 76:425-433
7. Bogdanovic R, Cobeljic M, Markovic M, Nikolic V, Ognjanovic M, Sarjanovic L, Makic D (1991) Haemolytic-uraemic syndrome associated with Aeromonas hydrophila enterocolitis. Ped Nephrol 5:293-295
8. Borrell N, Acinas SG, Figueras MJ, Martinez-Murcia AJ (1997) Identification of Aeromonas clinical isolates by restriction fragment length polymorphism of PCR-amplified 16S rRNA genes. J Clin Microbiol 35:1671-1674
9. Carnahan AM, Behram S, Joseph SW (1991) Aerokey II: a flexible key for identifying clinical Aeromonas species. J Clin Microbiol 29:2843-2849
10. Chopra AK, Houston CW (1999) Enterotoxins in Aeromonas-associated gastroenteritis. Microb Infect 1:1129-1137
11. Choresca CH, Gomez DK, Han JE, Shin SP, Kim JH, Jun JW, Park SC (2010) Molecular detection of Aeromonas hydrophila isolated from albino catfish, Clarias sp. reared in an indoor commercial aquarium. Korean J Vet Res 50:331-333
12. Devlieghere F, Lefevere I, Magnin A, Debevere J (2000) Growth of Aeromonas hydrophila in modified-atmosphere packed cooked meat products. Food Microbiol 17:185-196
13. Fontes MC, Saavedra MJ, Martins C, Martinez-Murcia AJ (2011) Phylogenetic identification of Aeromonas from pigs slaughtered for consumption in slaughterhouses at north of Portugal. Int J Food Microbiol 146:118-122
14. Gonzalez CJ, Santos JA, Garcia-Lopez ML, Gonzalez N, Otero A (2001) Mesophilic Aeromonas in wild and aquacultured freshwater fish. J Food Protect 64:687-691
15. Gonzalez-Rodriguez MN, Santos JA, Otera A, Lopez GML (2002) PCR detection of potentially pathogenic Aeromonas in raw and cold smoked freshwater fish. J Appl Microbiol 93:675-680
16. Haldar S, Majumdar SD, Chakravorty S, Tyagi JS, Bhalla M, Sen MK (2005) Detection of acid-fast bacilli in postlysis debris of clinical specimens improves the reliability of PCR. J Clin Microbiol 43:3580-3581
17. Haque QM, Sugiyama A, Iwade Y, Midorikawa Y, Yamauchi T (1996) Diarrheal and environmental isolates of Aeromonas spp. produce a toxin similar to Shiga-like toxin 1. Current Microbiol 32:239-245
18. Heuzenroeder MW, Wong CY, Flower RL (1999) Distribution of two hemolytic toxin genes in clinical and environmental isolates of Aeromonas spp.: correlation with virulence in a suckling mouse model. FEMS Microbiol Lett 174:131-136
19. Hirono I, Aoki T (1991) Nucleotide sequence and expression of an extracellular hemolysin gene of Aeromonas hydrophila. Microb Pathol 11:189-197
20. Howard SP, Garland WJ, Gree MJ, Buckley JT (1987) Nucleotide sequence of the gene for the hole-forming toxin aerolysin of Aeromonas hydrophila. J Bacteriol 169:2869-2871
21. Janda JM, Abbott SL (2010) The genus Aeromonas: taxonomy, pathogenicity, and infection. Clin Microbiol Rev 23:35-73
22. Janda JM, Kokka RP (1991) The pathogenicity of Aeromonas strains relative to genospecies and phenospecies identification. FEMS Microbiol Lett 15:29-33
23. Jeyasekaran G, Raj KT, Jeyashakila R, Thangarani AJ, Sukumar D (2011) Multiplex polymerase chain reaction based assay for the detection of toxin producing Vibrio cholerae in fish and fishery products. Appl Microbiol Biotech 90:1111-1118
24. Kirov SM (2001) Aeromonas and Plesiomonas species. In: Doyle MP, Beuchat LR, Montville TJ (eds) Food microbiology, fundamentals and frontiers, 2nd edn. ASM Press, Washington, DC, pp 301-327
25. Kumar S, Balakrishna K, Batra HV (2006) Detection of Salmonella enteric serovar Typhi (S. typhi) by selective amplification of invA, viaB, fliC-d and prt genes by polymerase chain reaction in multiplex format. Lett Appl Microbiol 42:149-154
26. Merino S, Rubires X, Knochel S, Thomas JM (1995) Emerging pathogens: Aeromonas spp. Int J Food Microbiol 28:157-168
27. Monfort P, Baleux B (1990) Dynamics of Aeromonas hydrophila, Aeromonas sobria and Aeromonas caviae in a sewage treatment pond. Appl Environ Microbiol 56:1999-2006
28. Nacescu N, Israil A, Cedru C, Caplan D (1992) Hemolytic properties of some Aeromonas strains. Roman Arch Microbiol Immunol 51:147-156
29. Nakano H, Kameyama T, Venkateswaran K, Kawakami H, Hashimoto H (1990) Distribution and characterization of hemolytic, and enteropathogenic motile Aeromonas in aquatic environment. Microbiol Immunol 34:447-458
30. Nam IY, Joh K (2007) Rapid detection of virulence factors of Aeromonas isolated from a trout farm by hexaplex PCR. J Microbiol 4:297-304
31. Pablos M, Rodriguez-Calleja JM, Santos JA, Otero A, Garcia-Lopez ML (2009) Occurence of motile Aeromonas in municipal drinking water and distribution of genes encoding virulence factors. Inter J Food Microbiol 135:158-164
32. Park TS, Oh SH, Lee EY, Lee TK, Park KH, Figueras MJ, Chang CL (2003) Misidentification of Aeromonas veronii biovar sobria as Vibrio alginolyticus by the Vitek system. Lett Appl Microbiol 37:349-353
33. Pin C, Benito Y, Garcia ML, Selgas D, Tormos J, Casas C (1996) Influence of temperature, pH, sodium chloride, and sodium nitrite on the growth of clinical and food motile Aeromonas spp. strains. Arch Lebens Hyg 47:35-56
34. Pinto AD, Terio V, Pinto PD, Tantillo G (2012) Detection of potentially pathogenic Aeromonas isolates from ready-to-eat seafood products by PCR analysis. Int J Food Sci Tech 47:269-273
35. Pollard DR, Johnson WM, Lior H, Tyler SD, Rozee KR (1990) Detection of the aerolysin gene in Aeromonas hydrophila by the polymerase chain reaction. J Clin Microbiol 28:2477-2481
36. Rahman M, Colque-Navarro P, Kuhn I, Huys G, Swings J, Mollby R (2002) Identification and characterization of pathogenic Aeromonas veronii biovar sobria associated with epizootic ulcerative syndrome in fish in Bangladesh. Appl Environ Microbiol 68:650-655
37. Raj KT, Jeyasekaran G, Jeyashakila R, Thangarani AJ, Sukumar D (2011) Multiplex polymerase chain reaction assay for the detection of Salmonella enteric serovars in shrimps in 4h. J Bacteriol Res 3:56-62
38. Robson WL, Leung AK, Trevenen CL (1992) Haemolytic-uraemic syndrome associated with Aeromonas hydrophila enterocolitis. Ped Nephrol 6:221-222
39. Sanchez TH, Brooks JT, Sullivan PS, Juhasz M, Mintz E, Dworkin MS (2005) Diarrhea in persons with HIV infection, United States, 1992-2002. Clin Infect Dis 41:1621-1627
40. Tsai MS, Kuo CY, Wang MC, Wu HC, Chien CC, Liu JW (2006) Clinical features and risk factors for mortality in Aeromonas bacteremic adults with hematologic malignancies. J Microbiol Immunol Infect 39:150-154
41. Vivekanandhan G, Hathab AAM, Lakshmanaperumalsamy P (2005) Prevalence of Aeromonas hydrophila in fish and prawns from the seafood market of Coimbatore, South India. Food Microbiol 22:133-137
42. Wang G, Clark CG, Liu C, Pucknell C, Munro CK, Kruk TMAC, Caldeira R, Woodward DL, Rodgers FG (2003) Detection and characterization of the hemolysin genes in Aeromonas hydrophila and Aeromonas sobria by multiplex PCR. J Clin Microbiol 41:1048-1054
43. Yanez MA, Catalan V, Apraiz D, Figueras MJ, Martinez-Murcia AJ (2003) Phylogenetic analysis of members of the genus Aeromonas based on gryB gene sequences. Int J Syst Evol Microbiol 53:875-883
44. Yogananth N, Bhakyaraj R, Chanthuru A, Anbalagan T, Nila KM (2009) Detection of virulence gene in Aeromonas hydrophila isolated from fish samples using PCR technique. Global J Biotechnol Biochem 4:51-53