Prolonged efficiency of siRNA-mediated gene silencing in primary cultures of human preadipocytes and adipocytes

Obesity

Volumn 22, Issue 4, 2014, Pages 1064-1069

  Lee, Mi Jeong ^a   Pickering, R Taylor ^a   Puri, Vishwajeet ^a  

^a BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCEROL; SMALL INTERFERING RNA;

ADIPOCYTE; ADIPOGENESIS; ADULT; ARTICLE; BETA ADRENERGIC STIMULATION; CELL DIFFERENTIATION; CELL FUNCTION; CONTROLLED STUDY; ENZYME RELEASE; GENE EXPRESSION; GENE REPRESSION; GENE SILENCING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN TISSUE; LIPOLYSIS; MICROSCOPY; PRIMARY CULTURE; PROADIPOCYTE; PROTEIN STRUCTURE; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SUBCUTANEOUS FAT; WESTERN BLOTTING; ADIPOSE TISSUE; CELL CULTURE; DRUG EFFECTS; EVALUATION STUDY; GENE EXPRESSION REGULATION; METABOLISM; MIDDLE AGED; PATHOLOGY; PHYSIOLOGY; STEM CELL; TIME;

ADIPOCYTES; ADIPOGENESIS; ADIPOSE TISSUE; ADULT; CELL DIFFERENTIATION; CELLS, CULTURED; GENE EXPRESSION REGULATION; GENE SILENCING; GLYCEROL; HUMANS; LIPOLYSIS; MIDDLE AGED; RNA, SMALL INTERFERING; STEM CELLS; TIME FACTORS;

EID: 84897456545     PISSN: 19307381     EISSN: 1930739X     Source Type: Journal    
DOI: 10.1002/oby.20641     Document Type: Article

References (34)

1. Fischer-Posovszky P, Newell FS, Wabitsch M, Tornqvist HE,. Human SGBS cells-a unique tool for studies of human fat cell biology. Obes Facts 2008; 1: 184-189.
2. Bordicchia M, Liu D, Amri EZ, et al. Cardiac natriuretic peptides act via p38 MAPK to induce the brown fat thermogenic program in mouse and human adipocytes. J Clin Invest 2012; 122: 1022-1036.
3. Hauner H, Wabitsch M, Pfeiffer EF,. Differentiation of adipocyte precursor cells from obese and nonobese adult women and from different adipose tissue sites. Horm Metab Res Suppl 1988; 19: 35-39.
4. Tchkonia T, Giorgadze N, Pirtskhalava T, et al. Fat depot-specific characteristics are retained in strains derived from single human preadipocytes. Diabetes 2006; 55: 2571-2578.
5. Tchkonia T, Giorgadze N, Pirtskhalava T, et al. Fat depot origin affects adipogenesis in primary cultured and cloned human preadipocytes. Am J Physiol Regul Integr Comp Physiol 2002; 282: R1286-R1296.
6. Tchkonia T, Tchoukalova YD, Giorgadze N, et al. Abundance of two human preadipocyte subtypes with distinct capacities for replication, adipogenesis, and apoptosis varies among fat depots. Am J Physiol Endocrinol Metab 2005; 288: E267-E277.
7. Tchkonia T, Giorgadze N, Pirtskhalava T, et al. Fat depot-specific characteristics are retained in strains derived from single human preadipocytes. Diabetes 2006; 55: 2571-2578.
8. Hauner H, Entenmann G,. Regional variation of adipose differentiation in cultured stromal-vascular cells from the abdominal and femoral adipose tissue of obese women. Int J Obes 1991; 15: 121-126.
9. Tchoukalova YD, Koutsari C, Votruba SB, et al. Sex- and depot-dependent differences in adipogenesis in normal-weight humans. Obesity 2010; 18: 1875-1880.
10. Isakson P, Hammarstedt A, Gustafson B, Smith U,. Impaired preadipocyte differentiation in human abdominal obesity: role of Wnt, tumor necrosis factor-alpha, and inflammation. Diabetes 2009; 58: 1550-1557.
11. Perez LM, Bernal A, San MN, Lorenzo M, Fernandez-Veledo S, Galvez BG,. Metabolic rescue of obese adipose-derived stems cells by Lin28/Let7 pathway. Diabetes 2013; 62: 2368-2379.
12. Sepe A, Tchkonia T, Thomou T, Zamboni M, Kirkland JL,. Aging and regional differences in fat cell progenitors-a mini-review. Gerontology 2011; 57: 66-75.
13. Lystedt E, Westergren H, Brynhildsen J, et al. Subcutaneous adipocytes from obese hyperinsulinemic women with polycystic ovary syndrome exhibit normal insulin sensitivity but reduced maximal insulin responsiveness. Eur J Endocrinol 2005; 153: 831-835.
14. Fried SK, Tittelbach T, Blumenthal J, et al. Resistance to the antilipolytic effect of insulin in adipocytes of African-American compared to Caucasian postmenopausal women. J Lipid Res 2010; 51: 1193-1200.
15. Lee MJ, Wu Y, Fried SK,. A modified protocol to maximize differentiation of human preadipocytes and improve metabolic phenotypes. Obesity 2012; 20: 2334-2340.
16. Lee MJ, Fried SK,. Glucocorticoids antagonize tumor necrosis factor-alpha-stimulated lipolysis and resistance to the antilipolytic effect of insulin in human adipocytes. Am J Physiol Endocrinol Metab 2012; 303: E1126-E1133.
17. Tiraby C, Tavernier G, Lefort C, et al. Acquirement of brown fat cell features by human white adipocytes. J Biol Chem 2003; 278: 33370-33376.
18. Pettersson AT, Laurencikiene J, Mejhert N, et al. A possible inflammatory role of twist1 in human white adipocytes. Diabetes 2010; 59: 564-571.
19. Bezaire V, Mairal A, Ribet C, et al. Contribution of adipose triglyceride lipase and hormone-sensitive lipase to lipolysis in hMADS adipocytes. J Biol Chem 2009; 284: 18282-18291.
20. Lee JY, Takahashi N, Yasubuchi M, et al. Triiodothyronine induces UCP-1 expression and mitochondrial biogenesis in human adipocytes. Am J Physiol Cell Physiol 2012; 302: C463-C472.
21. Puri V, Chakladar A, Virbasius JV, et al. RNAi-based gene silencing in primary mouse and human adipose tissues. J Lipid Res 2007; 48: 465-471.
22. Hauner H, Skurk T, Wabitsch M,. Cultures of human adipose precursor cells. Methods Mol Biol 2001; 155: 239-247.
23. Lee MJ, Wu Y, Fried SK,. A modified protocol to maximize differentiation of human preadipocytes and improve metabolic phenotypes. 426. Obesity 2012; 20: 2334-2340.
24. Mostoslavsky G, Kotton DN, Fabian AJ, Gray JT, Lee JS, Mulligan RC,. Efficiency of transduction of highly purified murine hematopoietic stem cells by lentiviral and oncoretroviral vectors under conditions of minimal in vitro manipulation. Mol Ther 2005; 11: 932-940.
25. Jambunathan S, Yin J, Khan W, Tamori Y, Puri V,. FSP27 Promotes lipid droplet clustering and then fusion to regulate triglyceride accumulation. PLoS One 2011; 6: e28614.
26. Xu Z, Yu S, Hsu CH, Eguchi J, Rosen ED,. The orphan nuclear receptor chicken ovalbumin upstream promoter-transcription factor II is a critical regulator of adipogenesis. Proc Natl Acad Sci U S A 2008; 105: 2421-2426.
27. Okamura M, Kudo H, Wakabayashi K, et al. COUP-TFII acts downstream of Wnt/beta-catenin signal to silence PPARgamma gene expression and repress adipogenesis. Proc Natl Acad Sci U S A 2009; 106: 5819-5824.
28. Puri V, Konda S, Ranjit S, et al. Fat-specific protein 27, a novel lipid droplet protein that enhances triglyceride storage. 375. J Biol Chem 2007; 282: 34213-34218.
29. Keller P, Petrie JT, De RP, et al. Fat-specific protein 27 regulates storage of triacylglycerol. J Biol Chem 2008; 283: 14355-14365.
30. Nishino N, Tamori Y, Tateya S, et al. FSP27 contributes to efficient energy storage in murine white adipocytes by promoting the formation of unilocular lipid droplets. J Clin Invest 2008; 118: 2808-2821.
31. Caprio M, Feve B, Claes A, Viengchareun S, Lombes M, Zennaro MC,. Pivotal role of the mineralocorticoid receptor in corticosteroid-induced adipogenesis. FASEB J 2007; 21: 2185-2194.
32. Tang X, Guilherme A, Chakladar A, et al. An RNA interference-based screen identifies MAP4K4/NIK as a negative regulator of PPARgamma, adipogenesis, and insulin-responsive hexose transport. Proc Natl Acad Sci U S A 2006; 103: 2087-2092.
33. Entenmann G, Hauner H,. Relationship between replication and differentiation in cultured human adipocyte precursor cells. Am J Physiol 1996; 270: C1011-C1016.
34. Skurk T, Ecklebe S, Hauner H,. A novel technique to propagate primary human preadipocytes without loss of differentiation capacity. Obesity 2007; 15: 2925-2931.