A modified protocol to maximize differentiation of human preadipocytes and improve metabolic phenotypes

Obesity

Volumn 20, Issue 12, 2012, Pages 2334-2340

  Lee, Mi Jeong ^a   Wu, Yuanyuan ^a   Fried, Susan K ^a  

^a BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADIPONECTIN; FATTY ACID BINDING PROTEIN 4; GLUCOSE; INSULIN; MESSENGER RNA; PERILIPIN; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; TRIACYLGLYCEROL;

ADIPOGENESIS; ADULT; ARTICLE; BETA ADRENERGIC STIMULATION; CELL DIFFERENTIATION; CELL STRUCTURE; CLINICAL ARTICLE; CONTROLLED STUDY; FABP4 GENE; GENE; GENE EXPRESSION REGULATION; GLUCOSE METABOLISM; GLUCOSE TRANSPORT; HUMAN; HUMAN CELL; HUMAN TISSUE; INSULIN SENSITIVITY; LIPOLYSIS; LONG TERM EXPOSURE; LPL GENE; OBESITY; PHENOTYPE; PPAR GAMMA GENE; PROADIPOCYTE; PROTEIN EXPRESSION; STROMA CELL;

ADIPOCYTES; ADIPOGENESIS; ADIPOSE TISSUE; ADULT; ANIMALS; CELL DIFFERENTIATION; CELLS, CULTURED; FEMALE; GENE EXPRESSION REGULATION; GLUCOSE; HUMANS; IMMUNOBLOTTING; INSULIN; LIPOLYSIS; MALE; PHENOTYPE; STROMAL CELLS;

BOVINAE;

EID: 84870294101     PISSN: 19307381     EISSN: 1930739X     Source Type: Journal    
DOI: 10.1038/oby.2012.116     Document Type: Article

References (31)

1. Djian P, Roncari AK, Hollenberg CH. Influence of anatomic site and age on the replication and differentiation of rat adipocyte precursors in culture. J Clin Invest 1983;72:1200-1208.
2. van Harmelen V, Skurk T, Röhrig K et al. Effect of BMI and age on adipose tissue cellularity and differentiation capacity in women. Int J Obes Relat Metab Disord 2003;27:889-895.
3. Hauner H, Entenmann G. Regional variation of adipose differentiation in cultured stromal-vascular cells from the abdominal and femoral adipose tissue of obese women. Int J Obes 1991;15:121-126.
4. Tchkonia T, Giorgadze N, Pirtskhalava T et al. Fat depot origin affects adipogenesis in primary cultured and cloned human preadipocytes. Am J Physiol Regul Integr Comp Physiol 2002;282:R1286-R1296.
5. Kirkland JL, Hollenberg CH, Kindler S, Gillon WS. Effects of age and anatomic site on preadipocyte number in rat fat depots. J Gerontol 1994;49:B31-B35.
6. Isakson P, Hammarstedt A, Gustafson B, Smith U. Impaired preadipocyte differentiation in human abdominal obesity: role of Wnt, tumor necrosis factor-alpha, and inflammation. Diabetes 2009;58:1550-1557.
7. Hauner H, Schmid P, Pfeiffer EF. Glucocorticoids and insulin promote the differentiation of human adipocyte precursor cells into fat cells. J Clin Endocrinol Metab 1987;64:832-835.
8. Skurk T, Ecklebe S, Hauner H. A novel technique to propagate primary human preadipocytes without loss of differentiation capacity. Obesity (Silver Spring) 2007;15:2925-2931.
9. Hauner H, Skurk T, Wabitsch M. Cultures of human adipose precursor cells. Methods Mol Biol 2001;155:239-247.
10. Yu G, Floyd ZE, Wu X et al. Adipogenic differentiation of adipose-derived stem cells. Methods Mol Biol 2011;702:193-200.
11. Fischer-Posovszky P, Newell FS, Wabitsch M, Tornqvist HE. Human SGBS cells-a unique tool for studies of human fat cell biology. Obes Facts 2008;1:184-189.
12. Dicker A, Rydén M, Näslund E et al. Effect of testosterone on lipolysis in human pre-adipocytes from different fat depots. Diabetologia 2004;47:420-428.
13. Skurk T, Hauner H. Primary culture of human adipocyte precursor cells: expansion and differentiation. Methods Mol Biol 2012;806:215-226.
14. Entenmann G, Hauner H. Relationship between replication and differentiation in cultured human adipocyte precursor cells. Am J Physiol 1996;270:C1011-C1016.
15. Shahparaki A, Grunder L, Sorisky A. Comparison of human abdominal subcutaneous versus omental preadipocyte differentiation in primary culture. Metab Clin Exp 2002;51:1211-1215.
16. Deslex S, Negrel R, Vannier C, Etienne J, Ailhaud G. Differentiation of human adipocyte precursors in a chemically defined serum-free medium. Int J Obes 1987;11:19-27.
17. Bunnell BA, Flaat M, Gagliardi C, Patel B, Ripoll C. Adiposederived stem cells: isolation, expansion and differentiation. Methods 2008;45:115-120.
18. Chazotte B. Labeling nuclear DNA using DAPI. Cold Spring Harb Protoc 2011 (1).
19. Honnor RC, Dhillon GS, Londos C. cAMP-dependent protein kinase and lipolysis in rat adipocytes. I. Cell preparation, manipulation, and predictability in behavior. J Biol Chem 1985;260:15122-15129.
20. Fried SK, Tittelbach T, Blumenthal J et al. Resistance to the antilipolytic effect of insulin in adipocytes of African-American compared to Caucasian postmenopausal women. J Lipid Res 2010;51:1193-1200.
21. Dole VP. A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J Clin Invest 1956;35:150-154.
22. Tchkonia T, Tchoukalova YD, Giorgadze N et al. Abundance of two human preadipocyte subtypes with distinct capacities for replication, adipogenesis, and apoptosis varies among fat depots. Am J Physiol Endocrinol Metab 2005;288:E267-E277.
23. Dicker A, Aström G, Sjölin E et al. The influence of preadipocyte differentiation capacity on lipolysis in human mature adipocytes. Horm Metab Res 2007;39:282-287.
24. Lystedt E, Westergren H, Brynhildsen J et al. Subcutaneous adipocytes from obese hyperinsulinemic women with polycystic ovary syndrome exhibit normal insulin sensitivity but reduced maximal insulin responsiveness. Eur J Endocrinol 2005;153:831-835.
25. Nagasaki H, Shang Q, Suzuki T et al. Low-serum culture system improves the adipogenic ability of visceral adipose tissue-derived stromal cells. Cell Biol Int 2011;35:559-568.
26. Deslex S, Negrel R, Ailhaud G. Development of a chemically defined serumfree medium for differentiation of rat adipose precursor cells. Exp Cell Res 1987;168:15-30.
27. Kuri-Harcuch W, Green H. Adipose conversion of 3T3 cells depends on a serum factor. Proc Natl Acad Sci USA 1978;75:6107-6109.
28. Schmidt W, Pöll-Jordan G, Löffler G. Adipose conversion of 3T3-L1 cells in a serum-free culture system depends on epidermal growth factor, insulin-like growth factor I, corticosterone, and cyclic AMP. J Biol Chem 1990;265:15489-15495.
29. Hauner H. Complete adipose differentiation of 3T3 L1 cells in a chemically defined medium: comparison to serum-containing culture conditions. Endocrinology 1990;127:865-872.
30. Pairault J, Green H. A study of the adipose conversion of suspended 3T3 cells by using glycerophosphate dehydrogenase as differentiation marker. Proc Natl Acad Sci USA 1979;76:5138-5142.
31. Kuri-Harcuch W, Marsch-Moreno M. DNA synthesis and cell division related to adipose differentiation of 3T3 cells. J Cell Physiol 1983;114:39-44.