N-terminal protein characterization by mass spectrometry after cyanogen bromide cleavage using combined microscale liquid- and solid-phase derivatization

Journal of Biomolecular Techniques

Volumn 25, Issue 1, 2014, Pages 19-30

  Nika, Heinz ^a   Hawke, David H ^b   Angeletti, Ruth Hogue ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

Author keywords

Covalent chromatography; N terminal peptide; Peptide identification; Reversed phase supports

Indexed keywords

CYANOGEN BROMIDE; N TERMINAL PEPTIDE; PEPTIDE; UNCLASSIFIED DRUG; PEPTIDE FRAGMENT; PROTEIN; SEPHAROSE; THIOL-SEPHAROSE;

ACETYLATION; ADSORPTION; AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; CHEMICAL MODIFICATION; CONTROLLED STUDY; DEACYLATION; DERIVATIZATION; DISULFIDE BOND; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN IMMOBILIZATION; PROTEIN ISOLATION; PROTEIN METHYLATION; PROTEIN PURIFICATION; THIOLATION; ANALOGS AND DERIVATIVES; CHEMISTRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR WEIGHT; PROCEDURES; SYNTHESIS;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYANOGEN BROMIDE; MOLECULAR WEIGHT; PEPTIDE FRAGMENTS; PEPTIDES; PROTEINS; PROTEOLYSIS; SEPHAROSE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84897424359     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: 10.7171/jbt.14-2501-003     Document Type: Article

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