Use of the heteroduplex mobility assay and cell sorting to select genome sequences of the CCR5 gene in HEK 293T cells edited by transcription activator-like effector nucleases

Genetics and Molecular Biology

Volumn 37, Issue 1, 2014, Pages 120-126

  Nerys Junior, Arildo ^a   Costa, Lendel C ^a   Braga Dias, Luciene P ^b   Oliveira, Márcia ^b   Rossi, Átila D ^a   da Cunha, Rodrigo Delvecchio ^a   Gonçalves, Gabriel S ^a   Tanuri, Amilcar ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b INSTITUTO OSWALDO CRUZ   (Brazil)

Author keywords

CCR5; Gene knockout; Genome editing; HMA; TALEN

Indexed keywords

CHEMOKINE RECEPTOR CCR5; HETERODUPLEX; NUCLEASE; TRANSCRIPTION ACTIVATOR LIKE EFFECTOR NUCLEASE; UNCLASSIFIED DRUG;

ARTICLE; CCR5 GENE; CELL LINE; CELL STRAIN HEK293T; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; FLUORESCENCE ACTIVATED CELL SORTING; GENE TARGETING; GENOME; HETERODUPLEX ANALYSIS; HETERODUPLEX MOBILITY ASSAY; HUMAN; MUTAGENESIS; SOMATIC MUTATION;

EID: 84897147555     PISSN: 14154757     EISSN: 16784685     Source Type: Journal    
DOI: 10.1590/S1415-47572014000100018     Document Type: Article

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