Genotyping of human parvovirus B19 in clinical samples from Brazil and Paraguay using heteroduplex mobility assay, single-stranded conformation polymorphism and nucleotide sequencing

Memorias do Instituto Oswaldo Cruz

Volumn 106, Issue 4, 2011, Pages 502-504

  de Mendonça, Marcos César Lima ^a   de Amorim Ferreira, Ana Maria ^a   dos Santos, Marta Gonçalves Matos ^a   Oviedo, Elva Cristina ^b   Dal Bello, Maria Sônia ^a   Siqueira, Marilda Mendonça ^c   Maceira, Juan Manuel Piñeiro ^d   von Hubinger, Maria Genoveva ^a   dos Santos Silva Couceiro, José Nelson ^a  

^a UNIVERSITY OF PASSO FUNDO   (Brazil)

^b LABORATORIO CENTRAL DE SALUD PÚBLICA   (Paraguay)

^c INSTITUTO OSWALDO CRUZ   (Brazil)

^d FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

Author keywords

Genotyping; HMA; Nucleotide sequencing; Parvovirus B19; SSCP

Indexed keywords

ARTICLE; BRAZIL; FEMALE; GENETICS; GENOTYPE; HETERODUPLEX ANALYSIS; HUMAN; HUMAN PARVOVIRUS B19; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PARAGUAY; PARVOVIRUS INFECTION; PREGNANCY; PREGNANCY COMPLICATION; SINGLE STRAND CONFORMATION POLYMORPHISM; VIROLOGY;

BASE SEQUENCE; BRAZIL; FEMALE; GENOTYPE; HETERODUPLEX ANALYSIS; HUMANS; MOLECULAR SEQUENCE DATA; PARAGUAY; PARVOVIRIDAE INFECTIONS; PARVOVIRUS B19, HUMAN; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; PREGNANCY; PREGNANCY COMPLICATIONS, INFECTIOUS;

PARECHOVIRUS; PARVOVIRUS;

EID: 79959516011     PISSN: 00740276     EISSN: 16788060     Source Type: Journal    
DOI: 10.1590/S0074-02762011000400019     Document Type: Article

References (13)

1. Barlow K, Green J, Clewley J 2000. Viral genome characterization by the heteroduplex mobility and heteroduplex tracking assays. Rev Med Virol 10: 321-335.
2. Cassinotti P, Weitz M, Siegl G 1993. Human parvovirus B19 infections: routine diagnosis by a new nested polymerase chain reaction assay. J Med Virol 40: 228-234.
3. Fauquet CM, Mayi MA, Maniloff J, Desselberger U, Ball L 2005. Virus taxonomy, 8th Report of the ICTV, Elsevier/Academic Press, London, 1259 pp.
4. Feder Jr, HM Anderson I 1989. Fifth disease: a brief review of infections in childhood, in adulthood and in pregnancy. Arch Intern Med 149: 2176-2179.
5. Freitas RB, Melo FL, Oliveira DS, Romano CM, Freitas MR, Macêdo O, Linhares AC, de A Zanotto PM, Durigon EL 2008. Molecular characterization of human erythrovirus B19 strains obtained from patients with several clinical presentations in the Amazon region of Brazil. J Clin Virol 43: 60-65.
6. Hayashi K, Yandell DW 1993. How sensitive is PCR-SSCP? Human Mut 2: 338-346.
7. Herring AJ, Inglis NF, Ojeh CK, Snodgrass DR, Menzies JD 1982. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol 16: 473-477.
8. Hübschen JM, Mihneva Z, Mentis AF, Schneider F, Aboudy Y, Grossman Z, Rudich H, Kasymbekova K, Sarv I, Nedeljkovic J, Tahita MC, Tarnagda Z, Ouedraogo JB, Gerasimova AG, Moskaleva TN, Tikhonova NT, Chitadze N, Forbi JC, Faneye AO, Otegbayo JA, Charpentier E, Muller CP 2009. Phylogenetic analysis of human parvovirus b19 sequences from eleven different countries confirms the predominance of genotype 1 and suggests the spread of genotype 3b. J Clin Microbiol 47: 3735-3738.
9. Keller LW, Barbosa ML, Melo FL, Pereira LM, David-Neto E, Lanhez LE, Durigon EL 2009. Phylogenetic analysis of a near-full-length sequence of an parvovirus B19 genotype 3 strain isolated in Brazil. Arch Virol 154: 1685-1687.
10. Kirchner JT 1994. Erythema infectiosum and other parvovirus B19 infections. Am Farm Phys 50: 335-342.
11. Mendonça MCL, Ferreira AMA, Santos MGM, Barros JJF, von Hubinger MG, Couceiro JNSS 2008. Heteroduplex mobility assay and single-stranded conformation polymorphism analysis as methodologies for detecting variants of human erythroviruses. J Virol Meth 148: 40-47.
12. Sanabani S, Kleine WN, Pereira J, Sabino EC 2006. Sequence variability of human parvovirus B19 present in bone marrow of brazilian patients with various parvovirus B19-related hematological symptoms. J Clin Microbiol 44: 604-606.
13. Servant A, Laperche S, Lallemand F, Marinho V, De Saint Maur G, Meritet JF, Garbarg-Chenon A 2002. Genetic diversity within human erythroviruses: identification of three genotypes. J Virol 76: 9124-9134.