Construction of a Kluyveromyces lactis ku80- host strain for recombinant protein production: Extracellular secretion of pectin lyase and a streptavidin-pectin lyase chimera

Molecular Biotechnology

Volumn 56, Issue 4, 2014, Pages 319-328

  Colombo, Lívia T ^a   Rosa, Júlio César C ^a   Bragança, Caio R S ^a   Ignacchiti, Raphael P ^a   Alvim, Mariana C T ^a   Silveira, Wendel B ^a   De Queiroz, Marisa V ^a   Bazzolli, Denise M S ^a   Passos, Flávia M L ^a  

^a FEDERAL UNIVERSITY OF VIÇOSA   (Brazil)

Author keywords

Homologous recombination; Kluyveromyces lactis; KU80; Split marker; Streptavidin pectin lyase

Indexed keywords

EFFICIENCY; PHYSIOLOGY; RECOMBINANT PROTEINS;

HOMOLOGOUS RECOMBINATION; KLUYVEROMYCES LACTIS; KU80; SPLIT-MARKER; STREPTAVIDIN-PECTIN LYASE;

GENE ENCODING;

FUNGAL DNA; HYBRID PROTEIN; PECTIN LYASE; RECOMBINANT PROTEIN; STREPTAVIDIN PECTIN LYASE FUSION PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CELLULAR SECRETION; CHIMERA; CONTROLLED STUDY; DNA END JOINING REPAIR; EXPERIMENTAL HOST; EXTRACELLULAR SPACE; FUNGAL GENE; FUNGAL GENOME; FUNGAL STRAIN; FUNGUS MUTANT; GENE CONSTRUCT; GENE DISRUPTION; GENE EXPRESSION; GENE VECTOR; GENETIC TRANSFORMATION; HOMOLOGOUS RECOMBINATION; KLUYVEROMYCES LACTIS; KU80 GENE; NONHUMAN; PROTEIN ENGINEERING; PROTEIN SECRETION;

KLUYVEROMYCES LACTIS;

DNA END-JOINING REPAIR; GENE EXPRESSION; KLUYVEROMYCES; POLYSACCHARIDE-LYASES; RECOMBINANT FUSION PROTEINS; STREPTAVIDIN;

EID: 84897096493     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-013-9711-3     Document Type: Article

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