Effect of long-term exposure to endodontic disinfecting solutions on young and old Enterococcus faecalis biofilms in dentin canals

Journal of Endodontics

Volumn 40, Issue 4, 2014, Pages 509-514

  Du, Tianfeng ^a,b   Wang, Zhejun ^b,c   Shen, Ya ^b   Ma, Jingzhi ^a   Cao, Yingguang ^a   Haapasalo, Markus ^b  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c Wuhan University   (China)

Author keywords

Biofilm; chlorhexidine; dentinal tubules; Enterococcus faecalis; sodium hypochlorite

Indexed keywords

BIOMEDICAL AND DENTAL MATERIALS; CHLORHEXIDINE; FLUORESCENT DYE; HYPOCHLORITE SODIUM; ORGANIC COMPOUND; PROPIDIUM IODIDE; SYTO 9; TOPICAL ANTIINFECTIVE AGENT; WATER;

BIOFILM; CONFOCAL MICROSCOPY; DENTAL PULP CAVITY; DENTIN; DRUG EFFECTS; ENTEROCOCCUS FAECALIS; HUMAN; MICROBIAL VIABILITY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; RANDOMIZATION; TIME FACTOR; ULTRASTRUCTURE;

ANTI-INFECTIVE AGENTS, LOCAL; BACTERIOLOGICAL TECHNIQUES; BIOFILMS; CHLORHEXIDINE; DENTAL PULP CAVITY; DENTIN; ENTEROCOCCUS FAECALIS; FLUORESCENT DYES; HUMANS; MICROBIAL VIABILITY; MICROSCOPY, CONFOCAL; ORGANIC CHEMICALS; PROPIDIUM; RANDOM ALLOCATION; ROOT CANAL IRRIGANTS; SODIUM HYPOCHLORITE; TIME FACTORS; WATER;

EID: 84897081399     PISSN: 00992399     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.joen.2013.11.026     Document Type: Article

References (35)

1. S. Kakehashi, H.R. Stanley, and R.J. Fitzgerald The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats Oral Surg Oral Med Oral Pathol 20 1965 340 349
2. R.S. Fulghum, C.B. Wiggins, and T.P. Mullaney Pilot study for detecting obligate anaerobic bacteria in necrotic dental pulps J Dent Res 52 1973 637
3. K. Keudell, M. Conte, and L. Fujimoto et al. Microorganisms isolated from pulp chambers J Endod 2 1976 146 148
4. A.J. Möller, L. Fabricius, and G. Dahlén et al. Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys Scand J Dent Res 89 1981 475 484
5. T. Waltimo, M. Trope, M. Haapasalo, and D. Orstavik Clinical efficacy of treatment procedures in endodontic infection control and one year follow-up of periapical healing J Endod 31 2005 863 866
6. I.N. Rôcas, and J.F. Siqueira Jr. Identification of bacteria enduring endodontic treatment procedures by a combined reverse transcriptase-polymerase chain reaction and reverse-capture checkerboard approach J Endod 36 2010 45 52
7. R.M. Love Invasion of dentinal tubules by root canal bacteria Endod Topics 9 2004 52 65
8. G. Dahlén, and G. Bergenholtz Endotoxic activity in teeth with necrotic pulps J Dent Res 59 1980 1033 1040
9. E.S. Akpata, and H. Blechman Bacterial invasion of pulpal dentin wall in vitro J Dent Res 61 1982 435 438
10. M. Haapasalo, and D. Orstavik In vitro infection and disinfection of dentinal tubules J Dent Res 66 1987 1375 1379
11. D. Orstavik, and M. Haapasalo Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules Endod Dental Traumatol 6 1990 142 149
12. R.M. Love Regional variation in root dentinal tubule infection by Streptococcus gordonii J Endod 22 1996 290 293
13. G. Kayaoglu, and D. Orstavik Virulence factors of Enterococcus faecalis: relationship to endodontic disease Crit Rev Oral Biol Med 15 2004 308 320
14. R.O. Zapata, C.M. Bramante, and I.G. de Moraes et al. Confocal laser scanning microscopy is appropriate to detect viability of Enterococcus faecalis in infected dentin J Endod 34 2008 1198 1201
15. D. Parmar, C.H. Hauman, and J.W. Leichter et al. Bacterial localization and viability assessment in human ex vivo dentinal tubules by fluorescence confocal laser scanning microscopy Int Endod J 44 2011 644 651
16. J. Ma, Z. Wang, Y. Shen, and M. Haapasalo A new noninvasive model to study the effectiveness of dentin disinfection by using confocal laser scanning microscopy J Endod 37 2011 1380 1385
17. Z. Wang, Y. Shen, J. Ma, and M. Haapasalo The effect of detergents on the antibacterial activity of disinfecting solutions in dentin J Endod 38 2012 948 953
18. Z. Wang, Y. Shen, and M. Haapasalo Effectiveness of endodontic disinfecting solutions against young and old Enterococcus faecalis biofilms in dentin canals J Endod 38 2012 1376 1379
19. I.M. Saleh, I.E. Ruyter, M. Haapasalo, and D. Orstavik Survival of Enterococcus faecalis in infected dentinal tubules after root canal filling with different root canal sealers in vitro Int Endod J 37 2004 193 198
20. J.C. Giard, J.M. Laplace, and A. Rincé et al. The stress proteome of Enterococcus faecalis Electrophoresis 22 2001 2947 2954
21. N. Ando, and E. Hoshino Predominant obligate anaerobes invading the deep layers of root canal dentine Int Endod J 23 1990 20 27
22. R.M. Love, and H.F. Jenkinson Invasion of dentinal tubules by oral bacteria Crit Rev Oral Biol Med 13 2002 171 183
23. G. Kayaoglu, H. Ömürlü, and G. Akca et al. Antibacterial activity of Propolis versus conventional endodontic disinfectants against Enterococcus faecalis in infected dentinal tubules J Endod 37 2011 376 381
24. S. Hecker, K.A. Hiller, and K.M. Galler et al. Establishment of an optimized ex vivo system for artificial root canal infection evaluated by use of sodium hypochlorite and the photodynamic therapy Int Endod J 46 2013 449 457
25. Z. Wang, Y. Shen, and M. Haapasalo Effect of smear layer against disinfection protocols on Enterococcus faecalis-infected dentin J Endod 39 2013 1395 1400
26. J. Cook, R. Nandakumar, and A.F. Fouad Molecular- and culture-based comparison of the effects of antimicrobial agents on bacterial survival in infected dentinal tubules J Endod 33 2007 690 692
27. I. Portenier, H. Haapasalo, and A. Rye et al. Inactivation of root canal medicaments by dentine, hydroxylapatite and bovine serum albumin Int Endod J 34 2001 184 188
28. P.A. Suci, and B.J. Tyler A method for discrimination of subpopulations of Candida albicans biofilm cells that exhibit relative levels of phenotypic resistance to chlorhexidine J Microbiol Methods 53 2003 313 325
29. L. Hall-Stoodley, J.W. Costerton, and P. Stoodley Bacterial biofilms: from the natural environment to infectious diseases Nat Rev Microbiol 2 2004 95 108
30. Y. Shen, S. Stojicic, and M. Haapasalo Bacterial viability in starved and revitalized biofilms: comparison of viability staining and direct culture J Endod 36 2010 1820 1823
31. Y. Shen, S. Stojicic, and M. Haapasalo Antimicrobial efficacy of chlorhexidine against bacteria in biofilms at different stages of development J Endod 37 2011 657 661
32. J. Xiao, and H. Koo Structural organization and dynamics of exopolysaccharide matrix and microcolonies formation by Streptococcus mutans in biofilms J Appl Microbiol 108 2010 2103 2113
33. B. Retamozo, S. Shabahang, and N. Johnson et al. Minimum contact time and concentration of sodium hypochlorite required to eliminate Enterococcus faecalis J Endod 36 2010 520 523
34. M. Marending, H.U. Luder, and T.J. Brunner et al. Effect of sodium hypochlorite on human root dentine-mechanical, chemical and structural evaluation Int Endod J 40 2007 786 793
35. T.P. Sim, J.C. Knowles, and Y.L. Ng et al. Effect of sodium hypochlorite on mechanical properties of dentine and tooth surface strain Int Endod J 34 2001 120 132