Identification of Bacteria Enduring Endodontic Treatment Procedures by a Combined Reverse Transcriptase-Polymerase Chain Reaction and Reverse-Capture Checkerboard Approach

Journal of Endodontics

Volumn 36, Issue 1, 2010, Pages 45-52

  Rôças, Isabela N ^a   Siqueira Jr , José F ^a  

^a Faculty of Dentistry   (Brazil)

Author keywords

Antibacterial treatment; apical periodontitis; persistent infection; reverse capture checkerboard DNA DNA hybridization; reverse transcriptase polymerase chain reaction

Indexed keywords

BACTERIAL DNA; BACTERIAL RNA; BIOMEDICAL AND DENTAL MATERIALS; CALCIUM HYDROXIDE; CAMPHOR; CAMPHORATED PARACHLOROPHENOL; CHLOROPHENOL; HYPOCHLORITE SODIUM; RNA 16S; ROOT CANAL FILLING MATERIAL;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIUM; CHRONIC PERIODONTITIS; DNA MICROARRAY; DRUG COMBINATION; DRUG EFFECT; ENDODONTICS; HUMAN; MICROBIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TOOTH PERIAPICAL DISEASE; TOOTH PULP;

BACTERIA; CALCIUM HYDROXIDE; CAMPHOR; CHLOROPHENOLS; CHRONIC PERIODONTITIS; DENTAL PULP CAVITY; DNA, BACTERIAL; DRUG COMBINATIONS; DRUG RESISTANCE, BACTERIAL; HUMANS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PERIAPICAL PERIODONTITIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; ROOT CANAL FILLING MATERIALS; ROOT CANAL IRRIGANTS; ROOT CANAL PREPARATION; SODIUM HYPOCHLORITE;

EID: 71049134871     PISSN: 00992399     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.joen.2009.10.022     Document Type: Article

References (40)

1. Siqueira Jr. J.F., and Rôças I.N. Clinical implications and microbiology of bacterial persistence after treatment procedures. J Endod 34 (2008) 1291-1301 e3
2. Byström A., and Sundqvist G. The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy. Int Endod J 18 (1985) 35-40
3. Sjögren U., Figdor D., Spangberg L., and Sundqvist G. The antimicrobial effect of calcium hydroxide as a short-term intracanal dressing. Int Endod J 24 (1991) 119-125
4. Peters L.B., van Winkelhoff A.J., Buijs J.F., and Wesselink P.R. Effects of instrumentation, irrigation and dressing with calcium hydroxide on infection in pulpless teeth with periapical bone lesions. Int Endod J 35 (2002) 13-21
5. 2. J Endod 31 (2005) 359-363
6. Siqueira Jr. J.F., Guimarães-Pinto T., and Rôças I.N. Effects of chemomechanical preparation with 2.5% sodium hypochlorite and intracanal medication with calcium hydroxide on cultivable bacteria in infected root canals. J Endod 33 (2007) 800-805
7. Siqueira Jr. J.F., Magalhães K.M., and Rôças I.N. Bacterial reduction in infected root canals treated with 2.5% NaOCl as an irrigant and calcium hydroxide/camphorated paramonochlorophenol paste as an intracanal dressing. J Endod 33 (2007) 667-672
8. Siqueira Jr. J.F., Paiva S.S., and Rôças I.N. Reduction in the cultivable bacterial populations in infected root canals by a chlorhexidine-based antimicrobial protocol. J Endod 33 (2007) 541-547
9. Sakamoto M., Siqueira Jr. J.F., Rôças I.N., and Benno Y. Bacterial reduction and persistence after endodontic treatment procedures. Oral Microbiol Immunol 22 (2007) 19-23
10. Carver K., Nusstein J., Reader A., and Beck M. In vivo antibacterial efficacy of ultrasound after hand and rotary instrumentation in human mandibular molars. J Endod 33 (2007) 1038-1043
11. Manzur A., Gonzalez A.M., Pozos A., Silva-Herzog D., and Friedman S. Bacterial quantification in teeth with apical periodontitis related to instrumentation and different intracanal medications: a randomized clinical trial. J Endod 33 (2007) 114-118
12. Vianna M.E., Horz H.P., Conrads G., Zaia A.A., Souza-Filho F.J., and Gomes B.P. Effect of root canal procedures on endotoxins and endodontic pathogens. Oral Microbiol Immunol 22 (2007) 411-418
13. Vianna M.E., Horz H.P., Conrads G., Feres M., and Gomes B.P.F.A. Comparative analysis of endodontic pathogens using checkerboard hybridization in relation to culture. Oral Microbiol Immunol 23 (2008) 282-290
14. Sjögren U., Figdor D., Persson S., and Sundqvist G. Influence of infection at the time of root filling on the outcome of endodontic treatment of teeth with apical periodontitis. Int Endod J 30 (1997) 297-306
15. Sundqvist G., Figdor D., Persson S., and Sjogren U. Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85 (1998) 86-93
16. Molander A., Warfvinge J., Reit C., and Kvist T. Clinical and radiographic evaluation of one- and two-visit endodontic treatment of asymptomatic necrotic teeth with apical periodontitis: a randomized clinical trial. J Endod 33 (2007) 1145-1148
17. Siqueira Jr. J.F., and Rôças I.N. Exploiting molecular methods to explore endodontic infections: part 1-current molecular technologies for microbiological diagnosis. J Endod 31 (2005) 411-423
18. McCarty S.C., and Atlas R.M. Effect of amplicon size on PCR detection of bacteria exposed to chlorine. PCR Methods Appl 3 (1993) 181-185
19. Keer J.T., and Birch L. Molecular methods for the assessment of bacterial viability. J Microbiol Methods 53 (2003) 175-183
20. McKillip J.L., Jaykus L.A., and Drake M. Nucleic acid persistence in heat-killed Escherichia coli O157:H7 from contaminated skim milk. J Food Prot 62 (1999) 839-844
21. Villarino A., Bouvet O.M., Regnault B., Martin-Delautre S., and Grimont P.A.D. Exploring the frontier between life and death in Escherichia coli: evaluation of different viability markers in live and heat- or UV-killed cells. Res Microbiol 151 (2000) 755-768
22. McKillip J.L., Jaykus L.A., and Drake M. rRNA stability in heat-killed and UV-irradiated enterotoxigenic Staphylococcus aureus and Escherichia coli O157:H7. Appl Environ Microbiol 64 (1998) 4264-4268
23. Cox C.J., Kempsell K.E., and Gaston J.S. Investigation of infectious agents associated with arthritis by reverse transcription PCR of bacterial rRNA. Arthritis Res Ther 5 (2003) R1-R8
24. Kempsell K.E., Cox C.J., Hurle M., et al. Reverse transcriptase-PCR analysis of bacterial rRNA for detection and characterization of bacterial species in arthritis synovial tissue. Infect Immun 68 (2000) 6012-6026
25. Miskin I.P., Farrimond P., and Head I.M. Identification of novel bacterial lineages as active members of microbial populations in a freshwater sediment using a rapid RNA extraction procedure and RT-PCR. Microbiology 145 Pt 8 (1999) 1977-1987
26. Ashimoto A., Chen C., Bakker I., and Slots J. Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions. Oral Microbiol Immunol 11 (1996) 266-273
27. Rôças I.N., and Siqueira Jr. J.F. Root canal microbiota of teeth with chronic apical periodontitis. J Clin Microbiol 46 (2008) 3599-3606
28. Paster B.J., Bartoszyk I.M., and Dewhirst F.E. Identification of oral streptococci using PCR-based, reverse-capture, checkerboard hybridization. Meth Cell Sci 20 (1998) 223-231
29. Rôças I.N., Hulsmann M., and Siqueira Jr. J.F. Microorganisms in root canal-treated teeth from a German population. J Endod 34 (2008) 926-931
30. van der Vliet G.M., Schepers P., Schukkink R.A., van Gemen B., and Klatser P.R. Assessment of mycobacterial viability by RNA amplification. Antimicrob Agents Chemother 38 (1994) 1959-1965
31. Kramer J.G., and Singleton F.L. Measurement of rRNA variations in natural communities of microorganisms on the southeastern U.S. continental shelf. Appl Environ Microbiol 59 (1993) 2430-2436
32. Kramer J.G., and Singleton F.L. Variations in rRNA content of marine Vibrio spp. during starvation-survival and recovery. Appl Environ Microbiol 58 (1992) 201-207
33. Teske A., Wawer C., Muyzer G., and Ramsing N.B. Distribution of sulfate-reducing bacteria in a stratified fjord (Mariager Fjord, Denmark) as evaluated by most-probable-number counts and denaturing gradient gel electrophoresis of PCR-amplified ribosomal DNA fragments. Appl Environ Microbiol 62 (1996) 1405-1415
34. Condon C., Squires C., and Squires C.L. Control of rRNA transcription in Escherichia coli. Microbiol Rev 59 (1995) 623-645
35. Siqueira Jr. J.F., Rôças I.N., Paiva S.S., Guimarães-Pinto T., Magalhães K.M., and Lima K.C. Bacteriologic investigation of the effects of sodium hypochlorite and chlorhexidine during the endodontic treatment of teeth with apical periodontitis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 104 (2007) 122-130
36. Gomes B.P., Lilley J.D., and Drucker D.B. Variations in the susceptibilities of components of the endodontic microflora to biomechanical procedures. Int Endod J 29 (1996) 235-241
37. Chavez de Paz L.E., Molander A., and Dahlen G. Gram-positive rods prevailing in teeth with apical periodontitis undergoing root canal treatment. Int Endod J 37 (2004) 579-587
38. Siqueira Jr. J.F., and Rôças I.N. The microbiota of acute apical abscesses. J Dent Res 88 (2009) 61-65
39. Downes J., Vartoukian S.R., Dewhirst F.E., et al. Pyramidobacter piscolens gen. nov., sp. nov., a member of the phylum 'Synergistetes' isolated from the human oral cavity. Int J Syst Evol Microbiol 59 (2009) 972-980
40. Schirrmeister J.F., Liebenow A.L., Pelz K., et al. New bacterial compositions in root-filled teeth with periradicular lesions. J Endod 35 (2009) 169-174