A new noninvasive model to study the effectiveness of dentin disinfection by using confocal laser scanning microscopy

Journal of Endodontics

Volumn 37, Issue 10, 2011, Pages 1380-1385

  Ma, Jingzhi ^a,b   Wang, Zhejun ^a,c   Shen, Ya ^a   Haapasalo, Markus ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^c WUHAN UNIVERSITY   (China)

Author keywords

Centrifugation; confocal laser scanning microscopy; dentin; disinfection; Enterococcus faecalis

Indexed keywords

BIGUANIDE DERIVATIVE; BIOMEDICAL AND DENTAL MATERIALS; CHLORHEXIDINE; HYPOCHLORITE SODIUM; POLYMER; QMIX ROOT CANAL IRRIGANT; TOPICAL ANTIINFECTIVE AGENT;

ARTICLE; BIOLOGICAL MODEL; CENTRIFUGATION; CONFOCAL MICROSCOPY; DENTIN; DRUG EFFECT; ENTEROCOCCUS FAECALIS; HUMAN; MICROBIOLOGY; TOOTH PULP; ULTRASTRUCTURE;

ANTI-INFECTIVE AGENTS, LOCAL; BIGUANIDES; CENTRIFUGATION; CHLORHEXIDINE; DENTAL PULP CAVITY; DENTIN; ENTEROCOCCUS FAECALIS; HUMANS; MICROSCOPY, CONFOCAL; MODELS, BIOLOGICAL; POLYMERS; ROOT CANAL IRRIGANTS; SODIUM HYPOCHLORITE;

EID: 80052913021     PISSN: 00992399     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.joen.2011.06.018     Document Type: Article

References (31)

1. S. Kakehashi, H.R. Stanley, and R.J. Fitzgerald The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats Oral Surg Oral Med Oral Pathol 20 1965 340 349
2. R.M. Love, N.P. Chandler, and H.F. Jenkinson Penetration of smeared or nonsmeared dentine by Streptococcus gordonii Int Endod J 29 1996 2 12 (Pubitemid 126445629)
3. Y. Shen, W. Qian, C. Chung, I. Olsen, and M. Haapasalo Evaluation of the effect of two chlorhexidine preparations on biofilm bacteria in vitro: a three-dimensional quantitative analysis J Endod 35 2009 981 985
4. J.R. Kuruvilla, and M.P. Kamath Antimicrobial activity of 2.5% sodium hypochlorite and 0.2% chlorhexidine gluconate separately and combined, as endodontic irrigants J Endod 24 1998 472 476
5. H.K. Haapasalo, E.K. Siren, T.M. Waltimo, D. Orstavik, and M.P. Haapasalo Inactivation of local root canal medicaments by dentine: an in vitro study Int Endod J 33 2000 126 131
6. I. Portenier, H. Haapasalo, and A. Rye Inactivation of root canal medicaments by dentine, hydroxylapatite and bovine serum albumin Int Endod J 34 2001 184 188 (Pubitemid 33671423)
7. I. Portenier, H. Haapasalo, D. Orstavik, M. Yamauchi, and M. Haapasalo Inactivation of the antibacterial activity of iodine potassium iodide and chlorhexidine digluconate against Enterococcus faecalis by dentin, dentin matrix, type-I collagen, and heat-killed microbial whole cells J Endod 28 2002 634 637
8. M. Haapasalo, W. Qian, I. Portenier, and T. Waltimo Effects of dentin on the antimicrobial properties of endodontic medicaments J Endod 33 2007 917 925
9. W.D. Miller The micro-organisms of the human mouth 1890 SS White Manufacturing Co Philadelphia
10. M. Haapasalo, and D. Orstavik In vitro infection and disinfection of dentinal tubules J Dent Res 66 1987 1375 1379 (Pubitemid 17127111)
11. R.O. Zapata, I.G. Moraes, and N. Bernardineli Confocal laser scanning microscopy is appropriate to detect viability of Enterococcus faecalis in infected dentin J Endod 34 2008 1198 1201
12. D. Parmar, C.H. Hauman, J.W. Leichter, A. McNaughton, and G.R. Tompkins Bacterial localization and viability assessment in human ex vivo dentinal tubules by fluorescence confocal laser scanning microscopy Int Endod J 44 2011 644 651
13. M. Nagayoshi, C. Kitamura, T. Fukuizumi, T. Nishihara, and M. Terashita Antimicrobial effect of ozonated water on bacteria invading dentinal tubules J Endod 30 2004 778 781 (Pubitemid 39481783)
14. Y. Shen, S. Stojicic, and M. Haapasalo Bacterial viability in starved and revitalized biofilms: comparison of viability staining and direct culture J Endod 36 2010 1820 1823
15. E.S. Akpata, and H. Blechman Bacterial invasion of pulpal dentin wall in vitro J Dent Res 61 1982 435 438
16. D. Orstavik, and M. Haapasalo Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules Endod Dent Traumatol 6 1990 142 149
17. D. Dymock, A.J. Weightman, C. Scully, and W.G. Wade Molecular analysis of microflora associated with dentoalveolar abscesses J Clin Microbiol 34 1996 537 542 (Pubitemid 26065771)
18. R.M. Love, and H.F. Jenkinson Invasion of dentinal tubules by oral bacteria Crit Rev Oral Biol Med 13 2002 171 183 (Pubitemid 34792655)
19. A. Reynaud af Geijersstam, R. Culak, and L. Molenaar Comparative analysis of virulence determinants and mass spectral profiles of Finnish and Lithuanian endodontic Enterococcus faecalis isolates Oral Microbiol Immunol 22 2007 87 94
20. J. Lehtinen, J. Nuutila, and E.M. Lilius Green fluorescent protein-propidium iodide (GFP-PI) based assay for flow cytometric measurement of bacterial viability Cytometry A 60 2004 165 172 (Pubitemid 39079509)
21. R.M. Love Invasion of dentinal tubules by root canal bacteria Endod Topics 9 2005 52 65
22. F.H. Takeda, T. Harashima, Y. Kimura, and K. Matsumoto A comparative study of the removal of smear layer by three endodontic irrigants and two types of laser Int Endod J 32 1999 32 39
23. Y. Shen, S. Stojicic, W. Qian, I. Olsen, and M. Haapasalo The synergistic antimicrobial effect by mechanical agitation and two chlorhexidine preparations on biofilm bacteria J Endod 36 2010 100 104
24. G. Villette, S. Manek, and M. Legner Characterization of an ex vivo model for the assessment of root canal disinfection J Endod 34 2008 1490 1496
25. L. Dai, K. Khechen, and S. Khan The effect of QMix, an experimental antibacterial root canal irrigant, on removal of canal wall smear layer and debris J Endod 37 2011 80 84
26. I. Portenier, T. Waltimo, D. Orstavik, and M. Haapasalo Killing of Enterococcus faecalis by MTAD and chlorhexidine digluconate with or without cetrimide in the presence or absence of dentine powder or BSA J Endod 32 2006 138 141 (Pubitemid 43309644)
27. M.T. Arias-Moliz, C.M. Ferrer-Luque, M.P. Gonzalez-Rodriguez, M.J. Valderrama, and P. Baca Eradication of Enterococcus faecalis biofilms by cetrimide and chlorhexidine J Endod 36 2010 87 90
28. A.M. Vacca-Smith, C.A. Jones, M.J. Levine, and M.W. Stinson Glucosyltransferase mediates adhesion of Streptococcus gordonii to human endothelial cells in vitro Infect Immun 62 1994 2187 2194 (Pubitemid 24157041)
29. J.F. Siqueira Jr., I.N. Rocas, A. Favieri, and K.C. Lima Chemomechanical reduction of the bacterial population in the root canal after instrumentation and irrigation with 1%, 2.5%, and 5.25% sodium hypochlorite J Endod 26 2000 331 334
30. B.P. Gomes, C.C. Ferraz, and M.E. Vianna In vitro antimicrobial activity of several concentrations of sodium hypochlorite and chlorhexidine gluconate in the elimination of Enterococcus faecalis Int Endod J 34 2001 424 428 (Pubitemid 33672085)
31. J.W. Harrison, and R.E. Hand The effect of dilution and organic matter on the anti-bacterial property of 5.25% sodium hypochlorite J Endod 7 1981 128 132