Venom present in sea anemone (Heteractis magnifica) induces apoptosis in non-small-cell lung cancer A549 cells through activation of mitochondria-mediated pathway

Biotechnology Letters

Volumn 36, Issue 3, 2014, Pages 489-495

  Ramezanpour, Mahnaz ^a   da Silva, Karen Burke ^b   Sanderson, Barbara J S ^a  

^a FLINDERS UNIVERSITY   (Australia)

^b FLINDERS UNIVERSITY   (Australia)

Author keywords

Apoptosis; Cancer cell lines (A549 and MRC5); Heteractis magnifica; Lung cancer; Mitochondria; Sea anemone; Venom

Indexed keywords

BIOLOGICAL ORGANS; CELL CULTURE; CELL DEATH; DISEASES; MITOCHONDRIA;

CANCER CELL LINES; HETERACTIS MAGNIFICA; LUNG CANCER; SEA ANEMONES; VENOM;

DRUG PRODUCTS;

ACTINIARIA; HETERACTIS MAGNIFICA;

CRYSTAL VIOLET; FORMAZAN; MTT FORMAZAN; TETRAZOLIUM; VENOM;

ANEMONE; ANIMAL; APOPTOSIS; ARTICLE; CELL SURVIVAL; DRUG EFFECT; EPITHELIUM CELL; HUMAN; METABOLISM; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION; STAINING; TUMOR CELL LINE;

ANEMONE; ANIMALS; APOPTOSIS; CELL LINE, TUMOR; CELL SURVIVAL; EPITHELIAL CELLS; FORMAZANS; GENTIAN VIOLET; HUMANS; MEMBRANE POTENTIAL, MITOCHONDRIAL; MITOCHONDRIA; STAINING AND LABELING; TETRAZOLIUM SALTS; VENOMS;

EID: 84897054082     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-013-1402-4     Document Type: Article

References (23)

1. Anderluh G, Maček P (2002) Cytolytic peptide and protein toxins from sea anemones (Anthozoa: Actiniaria). Toxicon 40: 111-124.
2. Elmore S (2007) Apoptosis: a review of programmed cell death. Toxicol Pathol 35: 495-516.
3. Fan T, Han L, Cong R, Liang J (2005) Caspase family proteases and apoptosis. Acta Biochim Biophys Sin (Shanghai) 37: 719-727.
4. Fedorov S, Dyshlovoy S, Monastyrnaya M, Shubina L et al (2010) The anticancer effects of actinoporin RTX-A from the sea anemone Heteractis crispa (=Radianthus macrodactylus). Toxicon 55: 811-817.
5. Fine A, Janssen-Heininger Y, Soultanakis R, Swisher S, Uhal B (2000) Apoptosis in lung pathophysiology. Am J Physiol Lung Cell Mol Physiol 279: L423-L427.
6. Kominsky D, Bickel R, Tyler K (2002) Reovirus-induced apoptosis requires both death receptor and mitochondrial-mediated caspase-dependent pathways of cell death. Cell Death Differ 9: 926-933.
7. Kroemer G (2003) Mitochondrial control of apoptosis: an introduction. Biochem Biophys Res Commun 304: 433-435.
8. Lanio ME, Morera V, Alvarez C, Tejuca M et al (2001) Purification and characterization of two hemolysins from Stichodactyla helianthus. Toxicon 39: 187-194.
9. Leal MC, Puga J, Serodio J, Gomes NCM, Calado R (2012) Trends in the discovery of new marine natural products from invertebrates over the last two decades-where and what are we bioprospecting? PLoS One 7: 30580.
10. Marino A, Valveri V, Muià C, Crupi R, Rizzo G, Musci G, Giuseppa LS (2004) Cytotoxicity of the nematocyst venom from the sea anemone Aiptasia mutabilis. Comp Biochem Physiol C 139: 295-301.
11. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63.
12. Penton D, Pérez-Barzaga V, Díaz I, Reytor M et al (2011) Validation of a mutant of the pore-forming toxin sticholysin-I for the construction of proteinase-activated immunotoxins. Protein Eng Des Sel 24: 485-493.
13. Ramezanpour M, Burke da Silva K, Sanderson BJ (2012) Differential susceptibilities of human lung, breast and skin cancer cell lines to killing by five sea anemone venoms. J Venom Anim Toxins 18: 157-163.
14. Saotome K, Morita H, Umeda M (1989) Cytotoxicity test with simplified crystal violet staining method using microtitre plates and its application to injection drugs. Toxicol In Vitro 3: 317-321.
15. Sencic L, Maček P (1990) New method for isolation of venom from the sea-anemone Actinia cari-purification and characterization of cytolytic toxins. Comp Biochem Phys B 97: 687-693.
16. Senderowicz AM (2003) Novel small molecule cyclin-dependent kinases modulators in human clinical trials. Cancer Biol Ther 2: 84-95.
17. Shivapurkar N, Reddy J, Chaudhary P, Gazdar A (2003) Apoptosis and lung cancer: a review. J Cell Biochem 88: 885-898.
18. Soletti RC, de Faria GP, Vernal J, Terenzi H, Anderluh G, Borges HL, Moura-Neto V, Gabilan NH (2008) Potentiation of anticancer-drug cytotoxicity by sea anemone pore-forming proteins in human glioblastoma cells. Anticancer Drugs 19: 517-525.
19. Standker L, Beress L, Garateix A, Christ T, Ravens U, Salceda E, Soto E, John H, Forssmanna W, Aneiros A (2006) A new toxin from the sea anemone Condylactis gigantea with effect on sodium channel inactivation. Toxicon 48: 211-220.
20. Tsujimoto Y, Shimizu S (2007) Role of the mitochondrial membrane permeability transition in cell death. Apoptosis 12: 835-840.
21. + channels. Toxicon 49: 550-560.
22. Uechi G, Toma H, Arakawa T, Sato Y (2005) Molecular cloning and functional expression of hemolysin from the sea anemone Actineria villosa. Protein Expr Purif 40: 379-384.
23. Young FM, Phungtamdet W, Sanderson BJ (2005) Modification of MTT assay conditions to examine the cytotoxic effects of amitraz on the human lymphoblastoid cell line, WIL2NS. Toxicol In Vitro 19: 1051-1059.