Purification and characterization of two hemolysins from Stichodactyla helianthus

Toxicon

Volumn 39, Issue 2-3, 2001, Pages 187-194

  Lanio, María Eliana ^a   Morera, Vivian ^b   Alvarez, Carlos ^a   Tejuca, Mayra ^a   Gómez, Teresita ^a   Pazos, Fabiola ^a   Besada, Vladimir ^b   Martínez, Diana ^a   Huerta, Vivian ^b   Padrón, Gabrìel ^b   Chávez, María De Los Angeles ^a  

^a UNIVERSITY OF HAVANA   (Cuba)

^b Protein Struct Dept Ctr G   (Cuba)

Author keywords

Cytolysin; Hemolysin; Sea anemone; Stichodactyla helianthus

Indexed keywords

ALANINE; AMINO ACID; CYSTEINE; CYTOLYSIN; GLYCINE; HEMOLYSIN; LEUCINE; LYSINE; PHOSPHOLIPASE; STICHOLYSIN I; STICHOLYSIN II; TYROSINE; UNCLASSIFIED DRUG; VALINE;

AMINO ACID COMPOSITION; AMINO TERMINAL SEQUENCE; CELL SUSPENSION; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ACTIVITY; ERYTHROCYTE; GEL FILTRATION CHROMATOGRAPHY; GENE INSERTION; HEMOLYSIS; HUMAN; HUMAN CELL; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PURIFICATION; SEA ANEMONE; SEQUENCE HOMOLOGY; STICHODACTYLA HELIANTHUS;

ACTINIARIA; ANEMONE; FELIS CATUS; HELIANTHUS; STICHODACTYLA HELIANTHUS;

EID: 0034598640     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0041-0101(00)00106-9     Document Type: Conference Paper

References (30)

1. Allen G. Amino acid analysis. Burdon R.H., van Knippenberg P.H. Laboratory Techniques in Biochemistry and Molecular Biology. Sequencing of Proteins and Peptides. 9:1989;40 Elsevier, Amsterdam, New York, Oxford.
2. Avila A.D., de Acosta M.C., Lage A. A new immunotoxin built by linking a hemolytic toxin to monoclonal antibodies for immature lymphocytes. Int. J. Cancer. 42:1988;568-571.
3. Belmonte G., Menestrina G., Pederzolli C., Krizaj I., Gubensek F., Turk T., Macek P. Primary and secondary structure of a pore-forming toxin from the sea anemone Actinia equina L., and its association with lipid vesicles. Biochim. Biophys. Acta. 1192:1994;197-204.
4. Belmonte G., Pederzolli C., Macek P., Menestrina G. Pore formation by the sea anemone cytolysin Equinatoxin II in the red blood cells and model lipid membranes. J. Membrane Biol. 131:1993;11-22.
5. Bernheimer A.W., Rudy B. Interaction between membranes and cytolytic peptides. Biochim. Biophys. Acta. 864:1986;123-141.
6. Blumenthal K.M., Kem W.R. Primary structure of Stoichactis helianthus cytolysin III. J. Biol. Chem. 258:1983;5574-5581.
7. ñeda O., Sotolongo V., Amor A.M., Stocklin R., Anderson A., Harvey A.L., Karlsson E. Characterization of a potassium channel blocker from Caribbean sea anemone Stichodactyla helianthus. Toxicon. 33:1995;603-613.
8. Cline E., Wolowyk M.W., Wiebe L.I., Rajan G., Samuel J. Isolation and characterization of a novel cardiac stimulatory and haemolytic protein from the sea anemone Urticina piscevora. Pharmaceutical Sciences. 1:1995;155-162.
9. Delfín J., González Y., Díaz J., Chávez M. Proteinase inhibitor from Stichodactyla helianthus: purification, characterization and immobilization. Archives of Medical Research. 25:1994;199-204.
10. Delfín J., Martínez I., Antuch W., Morera V., González Y., Rodríguez R., Márquez M., Saroyan A., Larionova N., Díaz J., Padrón G., Chávez M.A. Purification, characterization and immobilization of proteinase inhibitors from Stichodactyla helianthus. Toxicon. 34:1996;1367-1376.
11. Devlin J.P. Isolation and partial purification of hemolytic toxin from the sea anemone Stoichactis helianthus. J. Pharm. Sci. 63:1974;1478-1480.
12. Díaz A., Tejuca M., Alvarez C., Lanio M.E., Gómez T. Anticoagulant effect of total extract and some fractions obtained from the sea anemone Stichodactyla helianthus. Revista Iberoamericana de Trombosis y Homeostasis. 5:1992;8-11.
13. Galletis P., Norton R.S. Biochemical and pharmacological studies of the mechanism of action of Tenebrosin-C, a cardiac stimulatory and hemolytic protein from the sea anemone Actinia tenebrosa. Toxicon. 28:1990;695-700.
14. Gómez T., Romero D.L., Wong L., Barral A.M., Martínez J.R., Chávez M.A. Isolation and partial purification of two toxins and a protease inhibitor from Stoichactis helianthus anemone. Rev. Cubana Invest. Biom. 5:1986;117-125.
15. Kem W.R. sea anemone toxins: structure and action. Hessinger D.A, Lenhoff H.M. The Biology of the Nematocysts. 1988;375-405 Academic Press, New York.
16. Kem W.R., Dunn B.M. Separation and characterization of four different amino acid sequence variants of a sea anemone (Stichodactyla helianthus) protein cytolysin. Toxicon. 26:1988;997-1008.
17. Kem W.R., Parten B., Pennington M.W., Dunn B.M., Price D. Isolation, characterization and amino acid sequence of a polypeptide neurotoxic occurring in the sea anemone Stichodactyla helianthus. Biochem. 28:1989;3483-3489.
18. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
19. 2 activity by colorimetric assay using a pH indicator. Toxicon. 25:1987;1181-1188.
20. Lowry O.H., Rosenbrough H.J., Farr A.L., Randall R.J. Protein measurement with Folin phenol reagent. J. Biol. Chem. 193:1951;265-275.
21. Macek P. Polypeptide cytolytic toxins from sea anemones (Actinarias). FEMS Microbiol. Immun. 105:1992;121-130.
22. Macek P., Lebez D. Isolation and characterization of three lethal and hemolytic toxins from the sea anemone Actinia equina L. Toxicon. 26:1988;441-451.
23. Mebs D., Claus I., Schroter A., Takeya H., Iwanga S. Haemolytic proteins from sea anemones. Gopalakrishnakone P., Tan C.K. Recent Advances in Toxinology. 2:1992;392-395 Research National University of Singapore, Singapore.
24. Michaels D. Membrane damage by a toxin from the sea anemone Stoichactis helianthus. Biochim. Biophys. Acta. 555:1979;67-78.
25. 2 from sea anemone Stichodactyla helianthus. Revista Biología. 10:1996;115-123.
26. Pederzolli C., Belmonte G., Dallaserra M., Macek P., Menestrina G. Biochemical and cytotoxic properties of conjugates of transferrin with Equinatoxin II, a cytolysin from sea anemone. Bioconjugate Chemistry. 6:1995;166-173.
27. Sencic L., Macek P. New method for isolation of venom from the sea anemone Actinia cari. Purification and characterization of cytolytic toxins. Biochem. Physiol. 97B:1990;687-693.
28. Simpson R.J., Reid G.E., Moritz R.L., Morton C., Norton R.S. Complete amino acid sequence of Tenebrosin-C, cardiac and stimulatory and haemolytic protein from the sea anemone Actinia tenebrosa. Eur. J. Biochem. 190:1990;319-328.
29. Turk T. Cytolytic toxins from sea anemones. J. Toxicol. 10:1991;223-238.
30. Varanda W., Finkelstein A. Ion and nonelectrolyte permeability properties of channels formed in planar lipid bilayer membranes by cytolytic toxin from the sea anemone Stoichactis helianthus. J. Membrane Biol. 55:1980;203-211.