A new approach for discovering cold-active enzymes in a cell mixture of pure-cultured bacteria

Biotechnology Letters

Volumn 36, Issue 3, 2014, Pages 567-573

  Kim, Dockyu ^a   Park, Ha Ju ^a   Kim, Il Chan ^a   Yim, Joung Han ^a  

^a Korea Polar Research Institute (KOPRI)   (South Korea)

Author keywords

Activity based enzyme screen; Chitinase; Cold active; Culture mixture; Functional expression; Metagenomic library; Pseudoalteromonas issachenkonii

Indexed keywords

BACTERIA; CLONE CELLS; CLONING; ESCHERICHIA COLI; MIXTURES;

ACTIVITY-BASED; CHITINASES; COLD-ACTIVE; FUNCTIONAL EXPRESSION; METAGENOMIC LIBRARIES; PSEUDOALTEROMONAS;

ENZYME ACTIVITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOALTEROMONAS; PSEUDOALTEROMONAS ISSACHENKONII;

CHITINASE;

ARTICLE; CHEMISTRY; DNA SEQUENCE; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; MASS SCREENING; METABOLISM; METAGENOME; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PSEUDOALTEROMONAS; TEMPERATURE;

CHITINASE; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; MASS SCREENING; METAGENOME; MOLECULAR SEQUENCE DATA; PSEUDOALTEROMONAS; SEQUENCE ANALYSIS, DNA; TEMPERATURE;

EID: 84897042861     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-013-1384-2     Document Type: Article

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