Identification of a novel alkaliphilic esterase active at low temperatures by screening a metagenomic library from antarctic desert soil

Applied and Environmental Microbiology

Volumn 75, Issue 13, 2009, Pages 4657-4659

  Heath, Caroline ^a   Xiao, Ping Hu ^a   Cary, S Craig ^b   Cowan, Donald ^a  

^a UNIVERSITY OF THE WESTERN CAPE   (South Africa)

^b UNIVERSITY OF WAIKATO   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PH; AMINO ACID SEQUENCE; BURKHOLDERIA; DESERT SOILS; FUNCTIONAL SCREENING; LOW TEMPERATURES; METAGENOMIC LIBRARIES; TEMPERATURE RANGE;

AMINES; ESCHERICHIA COLI; INDUSTRIAL APPLICATIONS; SOILS;

LANDFORMS;

ESTERASE;

AMINO ACID; BACTERIUM; COLIFORM BACTERIUM; DESERT SOIL; ENZYME ACTIVITY; IDENTIFICATION METHOD; LOW TEMPERATURE; PH;

ALKALINITY; AMINO ACID SEQUENCE; ARTICLE; BURKHOLDERIA; BURKHOLDERIA XENOVORANS; DESERT; ENZYME ACTIVITY; ENZYME ASSAY; ESCHERICHIA COLI; METAGENOMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; SOIL;

ANTARCTIC REGIONS; BURKHOLDERIA; DNA; ENZYME STABILITY; ESCHERICHIA COLI; ESTERASES; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SOIL MICROBIOLOGY; TEMPERATURE;

BURKHOLDERIA XENOVORANS; ESCHERICHIA COLI;

EID: 67649609988     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02597-08     Document Type: Article

References (30)

1. Aislabie, J., K. Chhour, D. Saul, S. Miyauchi, J. Ayton, R. Paetzold, and M. Balks. 2006. Dominant bacteria in soils of Marble Point and Wright Valley, Victoria Land, Antarctica. Soil Biol. Biochem. 38:3041-3056.
2. Arpigny, J., and K. Jaeger. 1999. Bacterial lipolytic enzymes: classification and properties. Biochem. J. 343:177-183. (Pubitemid 29473893)
3. Aurilia, V., A. Parracino, and S. D'Auria. 2008. Microbial carbohydrate esterases in cold adapted environments. Gene 410:234-240.
4. Bell, P., A. Sunna, M. Gibbs, N. Curach, H. Nevalainen, and P. Bergquist. 2002. Prospecting for novel lipase genes using PCR. Microbiology 148:2283-2291. (Pubitemid 34928514)
5. Brady, L., A. M. Brzozowski, Z. S. Derewenda, E. Dodson, G. Dodson, S. Tolley, J. P. Turkenburg, L. Christiansen, B. Huge-Jensen, and L. Norskov. 1990. A serine protease triad forms the catalytic centre of a triacylglycerol lipase. Nature 343:767-770. (Pubitemid 120028611)
6. Cameron, R. E., J. King, and C. N. David. 1970. Microbial ecology and microclimatology of soil sites in dry valleys of southern Victoria Land, Antarctica. In M. W. Holdgate (ed.), Antarctic ecology. Academic Press, London, United Kingdom.
7. Choo, D., T. Kurihara, T. Suzuki, K. Soda, and N. Esaki. 1998. A coldadapted lipase of an Alaskan psychrotroph, Pseudomonas sp. strain B11-1: gene cloning and enzyme purification and characterization. Appl. Environ. Microbiol. 64:486-491.
8. Convey, P. 1996. The influence of environmental characteristics on life history attributes of Antarctic terrestrial biota. Biol. Rev. Camb. Philos. Soc. 71:191-225.
9. Cowan, D., N. Russell, A. Mamais, and D. Sheppard. 2002. Antarctic Dry Valley mineral soils contain unexpectedly high levels of microbial biomass. Extremophiles 6:431-436. (Pubitemid 41512701)
10. Derewenda, Z. S., and A. M. Sharp. 1993. News from the interface: the molecular structures of triacylglyceride lipases. Trends Biochem. Sci. 18:20-25.
11. Eisenthal, R., and A. Cornish-Bowden. 1974. The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters. Biochem. J. 139:715-720.
12. Ellis-Evans, J. C. 1997. Microscale distribution of photoautotrophic microorganisms in relation to light, temperature and moisture in Antarctic lithosols. In W. B. Lyons, C. Howard-Williams, and I. Hawes (ed.), Ecosystem processes in Antarctic ice-free landscapes. Balkema Press, Rotterdam, The Netherlands.
13. Hardeman, F., and S. Sjoling. 2007. Metagenomic approach for the isolation of a novel low-temperature-active lipase from uncultured bacteria of marine sediment. FEMS Microbiol. Ecol. 59:524-534.
14. Hirsch, P., W. Ludwig, C. Hethke, M. Sittig, B. Hoffmann, and C. A. Gallikowski. 1998. Hymenobacter roseosalivarius gen. nov., sp. nov. from continental Antarctic soils and sandstone bacterium of the Cytophaga- Flavobacterium- Bacteroidetes line of phylogenetic descent. Syst. Appl. Microbiol. 21:306-314.
15. Jaeger, K. E., S. Ransac, B. W. Dijkstra, C. Colson, M. Vanheuvel, and O. Misset. 1994. Bacterial lipases. FEMS Microbiol. Rev. 15:29-63.
16. Joseph, B., P. W. Ramteke, G. Thomas, and N. Shrivastava. 2007. Standard review cold-active microbial lipases: a versatile tool for industrial applications. Biotechnol. Mol. Biol. Rev. 2:39-48.
17. Kulakova, L., A. Galkin, T. Nakayama, T. Nishino, and N. Esaki. 2004. Cold-active esterase from Psychrobacter sp. Ant300: gene cloning, characterization, the effects of Gly-Pro substitution near the active site on its catalytic activity and stability. Biochim. Biophys. Acta 1696:59-65.
18. Mandrich, L., G. Manco, M. Rossi, E. Floris, and T. Jansen-van den Bosch. 2006. Alicyclobacillus acidocaldarius thermophilic esterase EST2's activity in milk and cheese models. Appl. Environ. Microbiol. 72:3191-3197.
19. Niederberger, T. D., I. R. McDonald, A. L. Hacker, R. M. Soo, J. E. Barrett, D. H. Wall, and S. C. Cary. 2008. Microbial community composition in soils of Northern Victoria Land, Antarctica. Environ. Microbiol. 10:1713-1724.
20. Ollis, D., E. Cheah, M. Cygler, B. W. Dijkstra, F. Frolow, S. Franken, M. Harel, S. Remington, I. Silman, and J. Schrag. 1992. The alpha/beta hydrolase fold. Protein Eng. 5:197-211.
21. Rashid, N., Y. Shimada, S. Ezaki, H. Atomi, and T. Imanaka. 2001. Lowtemperature lipase from psychrotrophic Pseudomonas sp. strain KB700A. Appl. Environ. Microbiol. 67:4064-4069.
22. Roh, C., and F. Villatte. 2008. Isolation of a low-temperature adapted lipolytic enzyme from uncultivated micro-organism. J. Appl. Microbiol. 105:116-123.
23. Smith, J. 2006. Microbial diversity and gene mining in Antarctic soils. Ph.D. thesis. University of the Western Cape, Cape Town, South Africa.
24. Smith, J., L. Tow, W. Stafford, C. Cary, and D. Cowan. 2006. Bacterial diversity in three different Antarctic Cold Desert mineral soils. Microb. Ecol. 51:413-421.
25. Wery, N., U. Gerike, A. Sharman, J. B. Chaudhuri, D. W. Hough, and M. J. Danson. 2003. Use of packed-column bioreactor for isolation of diverse protease-producing bacteria from Antarctic soil. Appl. Environ. Microbiol. 69:1457-1464.
26. Wynn-Williams, D. D. 1990. Ecological aspects of Antarctic microbiology. Adv. Microb. Ecol. 2:71-146.
27. Xu, Y., G. Feller, C. Gerday, and N. Glansdorff. 2003. Metabolic enzymes from psychrophilic bacteria: challenge of adaptation to low temperatures in ornithine carbamoyltransferase from Moritella abyssi. J. Bacteriol. 185:2161-2168. (Pubitemid 36363176)
28. Xu, Y., G. Feller, C. Gerday, and N. Glansdorff. 2003. Moritella cold-active dihydrofolate reductase: are there natural limits to optimization of catalytic efficiency at low temperature? J. Bacteriol. 185:5519-5526.
29. Yergeau, E., K. K. Newsham, D. A. Pearce, and G. A. Kowalchuk. 2007. Patterns of bacterial diversity across a range of Antarctic terrestrial habitats. Environ. Microbiol. 9:2670-2682.
30. Zhou, J., M. Bruns, and J. Tiedje. 1996. DNA recovery from soils of diverse composition. Appl. Environ. Microbiol. 62:316-322.