Sensitivity and specificity of ANA and anti-dsDNA in the diagnosis of systemic lupus erythematosus: A comparison using control sera obtained from healthy individuals and patients with multiple medical problems

Asian Pacific Journal of Allergy and Immunology

Volumn 31, Issue 4, 2013, Pages 292-298

  Wichainun, Ramjai ^a   Kasitanon, Nuntana ^a   Wangkaew, Suparaporn ^a   Hongsongkiat, Sith ^a   Sukitawut, Waraporn ^a   Louthrenoo, Worawit ^a  

^a CHIANG MAI UNIVERSITY   (Thailand)

Author keywords

ANA; Anti dsDNA; Lupus; Multi system disease; Sensitivity; Specificity

Indexed keywords

ANTINUCLEAR ANTIBODY; DOUBLE STRANDED DNA; AUTOANTIBODY; AUTOANTIGEN; DNA;

ADULT; AGED; ARTICLE; CONTROLLED STUDY; CRITHIDIA; CRITHIDIA LUCILIAE; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; IMMUNOFLUORESCENCE; MAJOR CLINICAL STUDY; MALE; MIDDLE AGED; PREVALENCE; RADIOIMMUNOASSAY; SENSITIVITY AND SPECIFICITY; SYSTEMIC LUPUS ERYTHEMATOSUS; BLOOD; COMPARATIVE STUDY; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOLOGY;

ADULT; ANTIBODIES, ANTINUCLEAR; AUTOANTIBODIES; AUTOANTIGENS; DNA; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HUMANS; LUPUS ERYTHEMATOSUS, SYSTEMIC; MALE; MIDDLE AGED; SENSITIVITY AND SPECIFICITY;

EID: 84897020130     PISSN: 0125877X     EISSN: 22288694     Source Type: Journal    
DOI: 10.12932/AP0272.31.4.2013     Document Type: Article

References (35)

1. Kumar Y, Bhatia A, Minz RW. Antinuclear antibodies and their detection methods in diagnosis of connective tissue diseases: a journey revisited. Diagn Pathol. 2009;4:1.
2. Kavanaugh A, Tomar R, Reveille J, Solomon DH, Homburger HA. Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. American College of Pathologists. Arch Pathol Lab Med. 2000;124:71-81.
3. Tozzoli R, Bizzaro N, Tonutti E, Villalta D, Bassetti D, Manoni F, et al. Guidelines for the laboratory use of autoantibody tests in the diagnosis and monitoring of autoimmune rheumatic diseases. Am J Clin Pathol. 2002;117:316-24.
4. Kasitanon N, Louthrenoo W, Sukitawut W, Vichainun R. Causes of death and prognostic factors in Thai patients with systemic lupus erythematosus. Asian Pac J Allergy Immunol. 2002;20:85-91.
5. Panicheewa S, Verasertniyom O, Chitrabamrung S, Vanichapuntu M, Vatanasuk M. Antinuclear antibodies in Thai patients with connective tissue diseases. Asian Pac J Allergy Immunol. 1987;5:47-52.
6. Marin GG, Cardiel MH, Cornejo H, Viveros ME. Prevalence of antinuclear antibodies in 3 groups of healthy individuals: blood donors, hospital personnel, and relatives of patients with autoimmune diseases. J Clin Rheumatol. 2009;15:325-9.
7. Nilsson BO, Skogh T, Ernerudh J, Johansson B, Lofgren S, Wikby A, et al. Antinuclear antibodies in the oldest-old women and men. J Autoimmun. 2006;27:281-8.
8. Tan EM, Feltkamp TE, Smolen JS, Butcher B, Dawkins R, Fritzler MJ, et al. Range of antinuclear antibodies in healthy individuals. Arthritis Rheum. 1997;40:1601-11.
9. Mariz HA, Sato EI, Barbosa SH, Rodrigues SH, Dellavance A, Andrade LE. Pattern on the antinuclear antibody-HEp-2 test is a critical parameter for discriminating antinuclear antibody-positive healthy individuals and patients with autoimmune rheumatic diseases. Arthritis Rheum. 2011;63:191-200.
10. Hochberg MC. Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1997;40:1725.
11. Greidinger EL, Hoffman RW. Antinuclear antibody testing: methods, indications, and interpretation. Lab Med 2003;34:113-7.
12. Keren DF. Antinuclear antibody testing. Clin Lab Med. 2002;22:447-74.
13. Bernardini S, Infantino M, Bellincampi L, Nuccetelli M, Afeltra A, Lori R, et al. Screening of antinuclear antibodies: comparison between enzyme immunoassay based on nuclear homogenates, purified or recombinant antigens and immunofluorescence assay. Clin Chem Lab Med. 2004;42:1155-60.
14. Gniewek RA, Stites DP, McHugh TM, Hilton JF, Nakagawa M. Comparison of antinuclear antibody testing methods: immunofluorescence assay versus enzyme immunoassay. Clin Diagn Lab Immunol. 1997;4:185-8.
15. Fritzler MJ, Wiik A, Tan EM, Smolen JS, McDougal JS, Chan EK, et al. A critical evaluation of enzyme immunoassay kits for detection of antinuclear autoantibodies of defined specificities. III. Comparative performance characteristics of academic and manufacturers' laboratories. J Rheumatol. 2003;30:2374-81.
16. Copple SS, Sawitzke AD, Wilson AM, Tebo AE, Hill HR. Enzymelinked immunosorbent assay screening then indirect immunofluorescence confirmation of antinuclear antibodies: a statistical analysis. Am J Clin Pathol. 2011;135:678-84.
17. Werle E, Blazek M, Fiehn W. The clinical significance of measuring different anti-dsDNA antibodies by using the Farr assay, an enzyme immunoassay and a Crithidia luciliae immunofluorescence test. Lupus. 1992;1:369-77.
18. Antico A, Platzgummer S, Bassetti D, Bizzaro N, Tozzoli R, Villalta D. Diagnosing systemic lupus erythematosus: newgeneration immunoassays for measurement of anti-dsDNA antibodies are an effective alternative to the Farr technique and the Crithidia luciliae immunofluorescence test. Lupus. 2010;19:906-12.
19. Pisetsky DS. Antinuclear antibodies in healthy people: the tip of autoimmunity's iceberg? Arthritis Res Ther. 2011;13:109.
20. Zarmbinski MA, Messner RP, Mandel JS. Anti-dsDNA antibodies in laboratory workers handling blood from patients with systemic lupus erythematosus. J Rheumatol. 1992;19:1380-4.
21. Arbuckle MR, McClain MT, Rubertone MV, Scofield RH, Dennis GJ, James JA, et al. Development of autoantibodies before the clinical onset of systemic lupus erythematosus. N Engl J Med. 2003;349:1526-33.
22. Wijeyesinghe U, Russell AS. Outcome of high titer antinuclear antibody positivity in individuals without connective tissue disease: a 10-year follow-up. Clin Rheumatol. 2008;27:1399-402.
23. Tan EM, Smolen JS, McDougal JS, Butcher BT, Conn D, Dawkins R, et al. A critical evaluation of enzyme immunoassays for detection of antinuclear autoantibodies of defined specificities. I. Precision, sensitivity, and specificity. Arthritis Rheum. 1999;42:455-64.
24. Riboldi P, Gerosa M, Moroni G, Radice A, Allegri F, Sinico A, et al. Anti-DNA antibodies: a diagnostic and prognostic tool for systemic lupus erythematosus? Autoimmunity. 2005;38:39-45
25. ter Borg EJ, Horst G, Hummel EJ, Limburg PC, Kallenberg CG. Measurement of increases in anti-double-stranded DNA antibody levels as a predictor of disease exacerbation in systemic lupus erythematosus. A long-term, prospective study. Arthritis Rheum. 1990;33:634-43.
26. Vedove CD, Del Giglio M, Schena D, Girolomoni G. Drug-induced lupus erythematosus. Arch Dermatol Res. 2009;301:99-105.
27. Vasoo S. Drug-induced lupus: an update. Lupus. 2006;15:757-61.
28. Finks SW, Finks AL, Self TH. Hydralazine-induced lupus: maintaining vigilance with increased use in patients with heart failure. South Med J. 2006;99:18-22.
29. Sarzi-Puttini P, Atzeni F, Capsoni F, Lubrano E, Doria A. Druginduced lupus erythematosus. Autoimmunity. 2005;38:507-18.
30. Imran A, Neelam F, Tariq M. Incidence of circulating antinuclear antibodies in cancer patients. Indian J Med Sci. 2003;57:113-6.
31. Solans-Laque R, Perez-Bocanegra C, Salud-Salvia A, Fonollosa-Pla V, Rodrigo MJ, Armadans L, et al. Clinical significance of antinuclear antibodies in malignant diseases: association with rheumatic and connective tissue paraneoplastic syndromes. Lupus. 2004;13:159-64.
32. Gregorio GV, Jones H, Choudhuri K, Vegnente A, Bortolotti F, Mieli-Vergani G, et al. Autoantibody prevalence in chronic hepatitis B virus infection: effect in interferon alfa. Hepatology. 1996;24:520-3.
33. Bayraktar Y, Bayraktar M, Gurakar A, Hassanein TI, Van Thiel DH. A comparison of the prevalence of autoantibodies in individuals with chronic hepatitis C and those with autoimmune hepatitis: the role of interferon in the development of autoimmune diseases. Hepatogastroenterology. 1997;44:417-25.
34. Cassani F, Cataleta M, Valentini P, Muratori P, Giostra F, Francesconi R, et al. Serum autoantibodies in chronic hepatitis C: comparison with autoimmune hepatitis and impact on the disease profile. Hepatology. 1997;26:561-6.
35. Calabrese LH. Autoimmune manifestations of human immunodeficiency virus (HIV) infection. Clin Lab Med. 1988;8:269-79