Functional expression of rat Nav1.6 voltage-gated sodium channels in HEK293 cells: Modulation by the auxiliary β1 subunit

PLoS ONE

Volumn 9, Issue 1, 2014, Pages

  He, Bingjun ^a   Soderlund, David M ^b  

^a NANKAI UNIVERSITY   (China)

^b Department of Obstetrics Gynecology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SODIUM; SODIUM CHANNEL NAV1.6 ALPHA SUBUNIT; SODIUM CHANNEL NAV1.6 BETA1 SUBUNIT; UNCLASSIFIED DRUG; VOLTAGE GATED SODIUM CHANNEL;

ARTICLE; CHANNEL GATING; CONTROLLED STUDY; DEPOLARIZATION; EMBRYO; HUMAN; HUMAN CELL; PROTEIN EXPRESSION; PROTEIN SUBUNIT; SODIUM CURRENT; STEADY STATE;

ANIMALS; GENE EXPRESSION; HEK293 CELLS; HUMANS; MEMBRANE POTENTIALS; NAV1.6 VOLTAGE-GATED SODIUM CHANNEL; PROTEIN SUBUNITS; RATS;

EID: 84896783830     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0085188     Document Type: Article

References (37)

1. Hille B (2001) Ion Channels of Excitable Membranes. Sunderland MA: Sinauer. 814 p.
2. Catterall WA (2000) From ionic currents to molecular mechanisms: structure and function of voltage-gated sodium channels. Neuron 26: 13-25.
3. + channel β subunits in development and disease. Neuroscience Letters 486: 53-59.
4. Goldin AL (2001) Resurgence of sodium channel research. Annual Review of Physiology 63: 871-894. (Pubitemid 32304340)
5. Yu FH, Catterall WA (2003) Overview of the voltage-gated sodium channel family. Genome Biology 4: 207.201-207.207.
6. Felts PA, Yokoyama S, Dib-Hajj S, Black JA, Waxman SG (1997) Sodium channel a-subunit mRNAs I, II, III, NaG, Na6 and hNE (PN1): different expression patters in developing rat nervous system. Molecular Brain Research 45: 71-82. (Pubitemid 27182226)
7. Whitaker WRJ, Clare JJ, Powell AJ, Chen YH, Faull RLM, et al. (2000) Distribution of voltage-gated sodium channel α-subunit and β-subunit mRNAs in human hippocampal formation, cortex, and cerebellum. Journal of Comparative Neurology 422: 123-139. (Pubitemid 30346568)
8. Whitaker WRJ, Faull RLM, Waldvogel HJ, Plumpton CJ, Emson PC, et al. (2001) Comparative distribution of voltage-gated sodium channel proteins in human brain. Molecular Brain Research 88: 37-53. (Pubitemid 32274258)
9. + channel α subunit with novel gating properties. Neuron 1: 449-461.
10. v1.6 is localized nodes of Ranvier, dendrites, and synapses. Proceedings of the National Academy of Sciences of the United States of America 97: 5616-5620. (Pubitemid 30313767)
11. v1.2 in action potential initiation and backpropagation. Nature Neuroscience 12: 996-1002.
12. Burgess DL, Kohrman DC, Galt J, Plummer NW, Jones JM, et al. (1995) Mutation of a new sodium channel gene, Scn8a, in the mouse mutant 'motor endplate disease'. Nature Genetics 10: 461-465.
13. Schaller KL, Caldwell JH (2003) Expression and distribution of voltage-gated sodium channels in the cerebellum. Cerebellum 2: 2-9. (Pubitemid 38281797)
14. Shah BS, Stevens EB, Pinnock RD, Dixon AK, Lee K (2001) Developmental expression of the novel voltage-gated sodium channel auxiliary subunit β3, in rat CNS. Journal of Physiology 534: 763-776. (Pubitemid 32700874)
15. v1.6 and β1 subunits in the coordinated regulation of excitability and neurite outgrowth. Proceedings of the National Academy of Sciences of the United States of America 107: 2283-2288.
16. Dietrich PS, McGivern JG, Delgado SG, Koch BD, Eglen RM, et al. (1998) Functional analysis of a voltage-gated sodium channel and its splice variant from rat dorsal root ganglia. Journal of Neurochemistry 70: 2262-2272. (Pubitemid 28252351)
17. v1.6 voltage-gated sodium channels by coexpression with the auxiliary β1 and β2 subunits. Biochemical and Biophysical Research Communications 407: 788-792.
18. Goldin AL (2006) Expression of ion channels in Xenopus oocytes. In: Clare JJ, Trezise DJ, editors. Expression and analysis of recombinant ion channels. Weinheim: Wiley VCH Verlag GmbH & Co. KgaA. 1-25.
19. Thomas P, Smart TG (2005) HEK293 cell line: a vehicle for the expression of recombinant proteins. Journal of Pharmacological and Toxicological Methods 51: 187-200. (Pubitemid 40615599)
20. v1.3 sodium channels. Pesticide Biochemistry and Physiology 101: 256-264.
21. He B, Soderlund DM (2011) Differential state-dependent modification of rat Nav1.6 sodium channels expressed in human embryonic kidney (HEK293) cells by the pyrethroid insecticides tefluthrin and deltamethrin. Toxicology and Applied Pharmacology 257: 377-387.
22. Bezanilla F, Armstrong CM (1977) Inactivation of the sodium channel. Journal of General Physiology 70: 549-566.
23. v1.6 voltagegated sodium channel from human brain. Molecular Brain Research 103: 80-90. (Pubitemid 34775036)
24. v1.6-mediated persistent current. Neuropharmacology 55: 1165-1171.
25. v1.6 sodium channels. Molecular and Cellular Neuroscience 38: 607-615.
26. v1.7 sodium channels. Neuroscience Letters 469: 268-272.
27. Khaliq ZM, Gouwens N, Raman IM (2003) The contribution of resurgent sodium current to high-frequency firing in Purkinje neurons: an experimental and modeling study. Journal of Neuroscience 23: 4899-4912. (Pubitemid 36793216)
28. Raman IM, Bean BP (2001) Inactivation and recovery of sodium currents in cerebellar Purkinje neurons: evidence for two mechanisms. Biophysical Journal 80: 729-737. (Pubitemid 32128326)
29. Graham FL, Smiley J, Russell WC, Nairn R (1977) Characteristics of a human cell line transformed by DNA from human adenovirus type 5. Journal of General Virology 36: 59-77.
30. Shaw G, Morse S, Ararat M, Graham FL (2002) Preferential transformation of human neuronal cells by human adeonviruses and the origin of HEK 293 cells. FASEB Journal 16: 869-871.
31. Moran O, Nizzari M, Conti F (2000) Endogenous expression of the b1A sodium channel subunit in HEK-293 cells. FEBS Letters 473: 132-134. (Pubitemid 30254275)
32. Moran O, Conti F, Tammaro P (2003) Sodium channel heterologous expression in mammalian cells and the endogenous beta-1 subunits. Neuroscience Letters 336: 175-179. (Pubitemid 36044606)
33. + channels by auxiliary β-subunits. Journal of Neurophysiology 106: 608-619.
34. Qu Y, Curtis R, Lawson D, Gilbride K, Ge P, et al. (2001) Differential modulation of sodium channel gating and persistent sodium currents of the β1, β2, and β3 subunits. Molecular and Cellular Neuroscience 18: 570-580. (Pubitemid 34008395)
35. v1.3 sodium channels: rapid repriming and slow closed-state inactivation display quantitative differences after expression in a mammalian cell line and in spinal sensory neurons. Journal of Neuroscience 21: 5952-5961. (Pubitemid 32737728)
36. Ferrera L, Moran O (2006) β1-subunit modulates the Nav1.4 sodium channel by changing the surface charge. Experimental Brain Research 172: 139-150. (Pubitemid 43854233)
37. v1.6-encoded sodium channels. Molecular and Cellular Neuroscience 42: 90-101.