Dramatically increased pH and temperature stability of chymotrypsin using dual block polymer-based protein engineering

Biomacromolecules

Volumn 15, Issue 3, 2014, Pages 763-771

  Cummings, Chad ^a   Murata, Hironobu ^b   Koepsel, Richard ^b   Russell, Alan J ^a,b  

^a CARNEGIE MELLON UNIVERSITY   (United States)

^b CARNEGIE MELLON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LOWER CRITICAL SOLUTION TEMPERATURE; N-ISOPROPYLACRYLAMIDES; PROTEASE DEGRADATION; PROTEIN-POLYMER CONJUGATES; TEMPERATURE STABILITY; TEMPERATURE-DEPENDENT CHANGES; TEMPERATURE-RESPONSIVE; UPPER CRITICAL SOLUTION TEMPERATURE;

ACRYLIC MONOMERS; ATOM TRANSFER RADICAL POLYMERIZATION; BIOCHEMICAL ENGINEERING; BIOSYNTHESIS; BLOCK COPOLYMERS; ENZYMES; GENETIC ENGINEERING; MOLECULAR WEIGHT;

POLYMERS;

CHYMOTRYPSIN; POLY(SULFOBETAINE METHACRYLAMIDE) BLOCK POLY(N ISOPROPYLACRYLAM; POLYMER; UNCLASSIFIED DRUG;

ARTICLE; ATOM TRANSFER RADICAL POLYMERIZATION; LOWER CRITICAL SOLUTION TEMPERATURE; PRIORITY JOURNAL; PROTEIN ENGINEERING; TEMPERATURE; THERMOSTABILITY; UPPER CRITICAL SOLUTION TEMPERATURE;

CHYMOTRYPSIN; HYDROGEN-ION CONCENTRATION; METHACRYLATES; POLYMERIZATION; POLYMERS; PROTEIN ENGINEERING; SOLUTIONS; TEMPERATURE; WATER;

EID: 84896771198     PISSN: 15257797     EISSN: 15264602     Source Type: Journal    
DOI: 10.1021/bm401575k     Document Type: Article

References (45)

1. Alconcel, S. N. S.; Baas, A. S.; Maynard, H. D. Polym. Chem. 2011, 2, 1442
2. Veronese, F. M. Biomaterials 2001, 22, 405
3. Hoffman, A. S.; Stayton, P. S. Prog. Polym. Sci. 2007, 32, 922
4. Lackey, C. A.; Murthy, N.; Press, O. W.; Tirrell, D. A.; Hoffman, A. S.; Stayton, P. S. Bioconjugate Chem. 1999, 10, 401
5. Zhang, Q.; Vanparijs, N.; Louage, B.; De Geest, B. G.; Hoogenboom, R. Polym. Chem. 2014, 5, 1140-1144
6. Strozyk, M. S.; Chanana, M.; Pastoriza-Santos, I.; Pérez-Juste, J.; Liz-Marzán, L. M. Adv. Funct. Mater. 2012, 22, 1436
7. Cummings, C.; Murata, H.; Koepsel, R.; Russell, A. J. Biomaterials 2013, 34, 7437
8. Keefe, A. J.; Jiang, S. Y. Nat. Chem. 2012, 4, 60
9. Fuhrmann, G.; Grotzky, A.; Lukic, R.; Matoori, S.; Luciani, P.; Yu, H.; Zhang, B.; Walde, P.; Schluter, A. D.; Gauthier, M. A.; Leroux, J. C. Nat. Chem. 2013, 5, 582
10. Murata, H.; Cummings, C. S.; Koepsel, R. R.; Russell, A. J. Biomacromolecules 2013, 14, 1919
11. Lele, B. S.; Murata, H.; Matyjaszewski, K.; Russell, A. J. Biomacromolecules 2005, 6, 3380
12. Nicolas, J.; San Miguel, V.; Mantovani, G.; Haddleton, D. M. Chem. Commun. 2006, 4697
13. Heredia, K. L.; Bontempo, D.; Ly, T.; Byers, J. T.; Halstenberg, S.; Maynard, H. D. J. Am. Chem. Soc. 2005, 127, 16955
14. Qi, Y.; Amiram, M.; Gao, W.; McCafferty, D. G.; Chilkoti, A. Macromol. Rapid Commun. 2013, 34, 1256
15. Gao, W.; Liu, W.; Mackay, J. A.; Zalutsky, M. R.; Toone, E. J.; Chilkoti, A. Proc. Natl. Acad. Sci. U.S.A. 2009, 106, 15231
16. Liu, J.; Bulmus, V.; Herlambang, D. L.; Barner-Kowollik, C.; Stenzel, M. H.; Davis, T. P. Angew. Chem., Int. Ed. 2007, 46, 3099
17. De, P.; Li, M.; Gondi, S. R.; Sumerlin, B. S. J. Am. Chem. Soc. 2008, 130, 11288
18. Naito, H.; Takewa, Y.; Mizuno, T.; Ohya, S.; Nakayama, Y.; Tatsumi, E.; Kitamura, S.; Takano, H.; Taniguchi, S.; Taenaka, Y. ASAIO J. 2004, 50, 344
19. Nelson, D. M.; Ma, Z.; Leeson, C. E.; Wagner, W. R. J. Biomed. Mater. Res., Part A 2012, 100A, 776
20. Zhou, P.; Yu, S. B.; Liu, Z. H.; Hu, J. M.; Deng, Y. Z. J. Chromatogr. A 2005, 1083, 173
21. Zhang, Z.; Finlay, J. A.; Wang, L.; Gao, Y.; Callow, J. A.; Callow, M. E.; Jiang, S. Langmuir 2009, 25, 13516
22. Smith, R. S.; Zhang, Z.; Bouchard, M.; Li, J.; Lapp, H. S.; Brotske, G. R.; Lucchino, D. L.; Weaver, D.; Roth, L. A.; Coury, A.; Biggerstaff, J.; Sukavaneshvar, S.; Langer, R.; Loose, C. Sci. Transl. Med. 2012, 4, 153ra132
23. Schild, H. G. Prog. Polym. Sci. 1992, 17, 163
24. Chen, L.; Honma, Y.; Mizutani, T.; Liaw, D. J.; Gong, J. P.; Osada, Y. Polymer 2000, 41, 141
25. Arotcarena, M.; Heise, B.; Ishaya, S.; Laschewsky, A. J. Am. Chem. Soc. 2002, 124, 3787
26. Weaver, J. V. M; Armes, S. P.; Butun, V. Chem. Commun. 2002, 2122
27. Kulkarni, S.; Schilli, C.; Muller, A. H. E.; Hoffman, A. S.; Stayton, P. S. Bioconjugate Chem. 2004, 15, 747
28. Boyer, C.; Bulmus, V.; Liu, J. Q.; Davis, T. P.; Stenzel, M. H.; Barner-Kowollik, C. J. Am. Chem. Soc. 2007, 129, 7145
29. Li, H. M.; Li, M.; Yu, X.; Bapat, A. P.; Sumerlin, B. S. Polym. Chem. 2011, 2, 1531
30. Li, M.; Li, H. M.; De, P.; Sumerlin, B. S. Macromol. Rapid Commun. 2011, 32, 354
31. Kulkarni, S.; Schilli, C.; Grin, B.; Muller, A. H. E.; Hoffman, A. S.; Stayton, P. S. Biomacromolecules 2006, 7, 2736
32. Hedstrom, L. Chem. Rev. 2002, 102, 4501
33. Kumar, A.; Venkatesu, P. Chem. Rev. 2012, 112, 4283
34. Turner, K. M.; Pasut, G.; Veronese, F. M.; Boyce, A.; Walsh, G. Biotechnol. Lett. 2011, 33, 617
35. Ciampolini, M.; Nardi, N. Inorg. Chem. 1966, 5, 41
36. Simakova, A.; Averick, S. E.; Konkolewicz, D.; Matyjaszewski, K. Macromolecules 2012, 45, 6371
37. Tsarevsky, N. V.; Pintauer, T.; Matyjaszewski, K. Macromolecules 2004, 37, 9768
38. Zhang, Y.; Furyk, S.; Sagle, L. B.; Cho, Y.; Bergbreiter, D. E.; Cremer, P. S. J. Phys. Chem. C 2007, 111, 8916
39. Rodríguez-Martínez, J. A.; Solá, R. J.; Castillo, B.; Cintrón-Colón, H. R.; Rivera-Rivera, I.; Barletta, G.; Griebenow, K. Biotechnol. Bioeng. 2008, 101, 1142
40. Reineke, J.; Cho, D. Y.; Dingle, Y. L.; Cheifetz, P.; Laulicht, B.; Lavin, D.; Furtado, S.; Mathiowitz, E. J. Controlled Release 2013, 170, 477
41. Xu, Q.; Boylan, N. J.; Cai, S.; Miao, B.; Patel, H.; Hanes, J. J. Controlled Release 2013, 170, 279
42. Baldwin, R. L. Biophys. J. 1996, 71, 2056
43. Chen, J. P.; Hoffman, A. S. Biomaterials 1990, 11, 631
44. Callahan, D. J.; Liu, W. E.; Li, X. H.; Dreher, M. R.; Hassouneh, W.; Kim, M.; Marszalek, P.; Chilkoti, A. Nano Lett. 2012, 12, 2165
45. McDaniel, J. R.; Dewhirst, M. W.; Chilkoti, A. Int. J. Hyperthermia 2013, 29, 501