Tailoring enzyme activity and stability using polymer-based protein engineering

Biomaterials

Volumn 34, Issue 30, 2013, Pages 7437-7443

  Cummings, Chad ^a   Murata, Hironobu ^b   Koepsel, Richard ^b   Russell, Alan J ^a,b  

^a CARNEGIE MELLON UNIVERSITY   (United States)

^b CARNEGIE MELLON UNIVERSITY   (United States)

Author keywords

Atom transfer radical polymerization; Chymotrypsin; Enzyme; Enzyme polymer conjugates; Protein engineering

Indexed keywords

CHYMOTRYPSIN; ENZYME-POLYMER CONJUGATES; POLY (N ISOPROPYLACRYLAMIDE); PROTEIN ENGINEERING; TEMPERATURE DEPENDENT; TEMPERATURE-DEPENDENT CHANGES; TEMPERATURE-RESPONSIVE; WATER-SOLUBLE INITIATORS;

ACRYLIC MONOMERS; AMINO ACIDS; ATOM TRANSFER RADICAL POLYMERIZATION; BIOCHEMICAL ENGINEERING; BIOSYNTHESIS; CONFORMATIONS; ENZYME KINETICS; ENZYMES; GENETIC ENGINEERING; MOLECULAR BIOLOGY; POLYMERIZATION; STABILITY;

POLYMERS;

CHYMOTRYPSIN; LYSINE; POLY(N ISOPROPYLACRYLAMIDE); POLY[N,N' DIMETHYL(METHACRYLOYLETHYL)AMMONIUM PROPANE SULFONATE]; POLYMER; UNCLASSIFIED DRUG;

ARTICLE; ATOM TRANSFER RADICAL POLYMERIZATION; BIOLOGICAL ACTIVITY; CONFORMATION; CONJUGATE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STABILITY; ENZYME STRUCTURE; GEL PERMEATION CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; PH; POLYMER BASED PROTEIN ENGINEERING; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ENGINEERING; ROOM TEMPERATURE; SYNTHESIS; TEMPERATURE; TEMPERATURE DEPENDENCE;

ATOM TRANSFER RADICAL POLYMERIZATION; CHYMOTRYPSIN; ENZYME; ENZYME-POLYMER CONJUGATES; PROTEIN ENGINEERING;

ACRYLIC RESINS; ANIMALS; CATTLE; CHYMOTRYPSIN; ENZYME ACTIVATION; ENZYME STABILITY; HYDROLYSIS; KINETICS; METHACRYLATES; POLYMERIZATION; POLYMERS; PROTEIN ENGINEERING; QUATERNARY AMMONIUM COMPOUNDS; TEMPERATURE;

EID: 84880509608     PISSN: 01429612     EISSN: 18785905     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2013.06.027     Document Type: Article

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