Good manufacturing practice-compliant isolation and culture of human umbilical cord blood-derived mesenchymal stem cells

Journal of Translational Medicine

Volumn 12, Issue 1, 2014, Pages

  Pham, Phuc Van ^a   Vu, Ngoc Bich ^a   Pham, Vuong Minh ^a   Truong, Nhung Hai ^a   Pham, Truc Le Buu ^a   Dang, Loan Thi Tung ^a   Nguyen, Tam Thanh ^a   Bui, Anh Nguyen Tu ^a   Phan, Ngoc Kim ^a  

^a UNIVERSITY OF NATURAL SCIENCES   (Viet Nam)

Author keywords

Clinical application; Good manufacturing practice; Mesenchymal stem cells; Platelet rich plasma; Umbilical cord blood

Indexed keywords

ANIMAL EXPERIMENT; ARTICLE; BLOOD SAMPLING; CELL ADHESION; CELL DIFFERENTIATION; CELL ISOLATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE MEDIUM; GOOD MANUFACTURING PRACTICE; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; IN VITRO STUDY; MESENCHYMAL STEM CELL; MOUSE; NONHUMAN; PROTEIN EXPRESSION; STEM CELL CULTURE; STEM CELL EXPANSION; THROMBOCYTE RICH PLASMA; UMBILICAL CORD BLOOD;

ADIPOCYTES; ANIMALS; CARCINOGENESIS; CATTLE; CELL ADHESION; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SEPARATION; CELL SHAPE; CELLS, CULTURED; CULTURE MEDIA; FETAL BLOOD; GUIDELINE ADHERENCE; HUMANS; IMMUNOPHENOTYPING; MESENCHYMAL STROMAL CELLS; MICE; MICE, NUDE; OSTEOBLASTS; TIME FACTORS;

EID: 84896701683     PISSN: None     EISSN: 14795876     Source Type: Journal    
DOI: 10.1186/1479-5876-12-56     Document Type: Article

References (64)

1. Friedenstein AJ, Piatetzky S, Petrakova KV. Osteogenesis in transplants of bone marrow cells. J Embryol Exp Morphol 1966, 16:381-390.
2. Friedenstein AJ, Deriglasova UF, Kulagina NN, Panasuk AF, Rudakowa SF, Luria EA, Ruadkow IA. Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method. Exp Hematol 1974, 2:83-92.
3. Prockop DJ, Sekiya I, Colter DC. Isolation and characterization of rapidly self-renewing stem cells from cultures of human marrow stromal cells. Cytotherapy 2001, 3:393-396. 10.1080/146532401753277229, 11953019.
4. Jones EA, Kinsey SE, English A, Jones RA, Straszynski L, Meredith DM, Markham AF, Jack A, Emery P, McGonagle D. Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells. Arthritis Rheum 2002, 46:3349-3360. 10.1002/art.10696, 12483742.
5. Bunnell BA, Flaat M, Gagliardi C, Patel B, Ripoll C. Adipose-derived stem cells: isolation, expansion and differentiation. Methods 2008, 45:115-120. 10.1016/j.ymeth.2008.03.006, 3668445, 18593609.
6. Boquest AC, Shahdadfar A, Brinchmann JE, Collas P. Isolation of stromal stem cells from human adipose tissue. Methods Mol Biol 2006, 325:35-46.
7. Van Pham P, Bui KH, Ngo DQ, Vu NB, Truong NH, Phan NL, Le DM, Duong TD, Nguyen TD, Le VT, Phan NK. Activated platelet-rich plasma improves adipose-derived stem cell transplantation efficiency in injured articular cartilage. Stem Cell Res Ther 2013, 4:91. 10.1186/scrt277, 3854675, 23915433.
8. Bieback K, Kern S, Kluter H, Eichler H. Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood. Stem Cells 2004, 22:625-634. 10.1634/stemcells.22-4-625, 15277708.
9. Lee OK, Kuo TK, Chen WM, Lee KD, Hsieh SL, Chen TH. Isolation of multipotent mesenchymal stem cells from umbilical cord blood. Blood 2004, 103:1669-1675. 10.1182/blood-2003-05-1670, 14576065.
10. Mareschi K, Biasin E, Piacibello W, Aglietta M, Madon E, Fagioli F. Isolation of human mesenchymal stem cells: bone marrow versus umbilical cord blood. Haematologica 2001, 86:1099-1100.
11. Phuc PV, Nhung TH, Loan DT, Chung DC, Ngoc PK. Differentiating of banked human umbilical cord blood-derived mesenchymal stem cells into insulin-secreting cells. In Vitro Cell Dev Biol Anim 2011, 47:54-63. 10.1007/s11626-010-9356-5, 21082287.
12. Phuc PV, Ngoc VB, Lam DH, Tam NT, Viet PQ, Ngoc PK. Isolation of three important types of stem cells from the same samples of banked umbilical cord blood. Cell Tissue Bank 2012, 13:341-351. 10.1007/s10561-011-9262-4, 21656022.
13. Gong W, Han Z, Zhao H, Wang Y, Wang J, Zhong J, Wang B, Wang S, Wang Y, Sun L, Han Z. Banking human umbilical cord-derived mesenchymal stromal cells for clinical use. Cell Transplant 2012, 21:207-216.
14. Matsuo A, Yamazaki Y, Takase C, Aoyagi K, Uchinuma E. Osteogenic potential of cryopreserved human bone marrow-derived mesenchymal stem cells cultured with autologous serum. J Craniofac Surg 2008, 19:693-700. 10.1097/SCS.0b013e318163f2cc, 18520385.
15. Romanov YA, Svintsitskaya VA, Smirnov VN. Searching for alternative sources of postnatal human mesenchymal stem cells: candidate MSC-like cells from umbilical cord. Stem Cells 2003, 21:105-110. 10.1634/stemcells.21-1-105, 12529557.
16. Wang HS, Hung SC, Peng ST, Huang CC, Wei HM, Guo YJ, Fu YS, Lai MC, Chen CC. Mesenchymal stem cells in the Wharton's jelly of the human umbilical cord. Stem Cells 2004, 22:1330-1337. 10.1634/stemcells.2004-0013, 15579650.
17. In 't Anker PS, Scherjon SA, Kleijburg-van der Keur C, de Groot-Swings GM, Claas FH, Fibbe WE, Kanhai HH. Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta. Stem Cells 2004, 22:1338-1345. 10.1634/stemcells.2004-0058, 15579651.
18. Roubelakis MG, Pappa KI, Bitsika V, Zagoura D, Vlahou A, Papadaki HA, Antsaklis A, Anagnou NP. Molecular and proteomic characterization of human mesenchymal stem cells derived from amniotic fluid: comparison to bone marrow mesenchymal stem cells. Stem Cells Dev 2007, 16:931-952. 10.1089/scd.2007.0036, 18047393.
19. Perry BC, Zhou D, Wu X, Yang FC, Byers MA, Chu TM, Hockema JJ, Woods EJ, Goebel WS. Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use. Tissue Eng Part C Methods 2008, 14:149-156. 2963629, 18489245.
20. Ulrich D, Muralitharan R, Gargett CE. Toward the use of endometrial and menstrual blood mesenchymal stem cells for cell-based therapies. Expert Opin Biol Ther 2013, 13:1387-1400. 10.1517/14712598.2013.826187, 23930703.
21. Verina T, Fatemi A, Johnston MV, Comi AM. Pluripotent possibilities: human umbilical cord blood cell treatment after neonatal brain injury. Pediatr Neurol 2013, 48:346-354. 10.1016/j.pediatrneurol.2012.10.010, 23583051.
22. Chung WH, Park SA, Lee JH, Chung DJ, Choi CB, Kim DH, Han H, Kim HY. Percutaneous transplantation of human umbilical cord-derived mesenchymal stem cells in a dog with suspected fibrocartilaginous embolic myelopathy. J Vet Sci 2013, 14(4):495-497. 10.4142/jvs.2013.14.4.495, 3885746, 23820160.
23. Lee JH, Chang HS, Kang EH, Chung DJ, Choi CB, Lee JH, Hwang SH, Han H, Kim HY. Percutaneous transplantation of human umbilical cord blood-derived multipotent stem cells in a canine model of spinal cord injury. J Neurosurg Spine 2009, 11:749-757. 10.3171/2009.6.SPINE08710, 19951029.
24. Roh DH, Seo MS, Choi HS, Park SB, Han HJ, Beitz AJ, Kang KS, Lee JH. Transplantation of human umbilical cord blood or amniotic epithelial stem cells alleviates mechanical allodynia after spinal cord injury in rats. Cell Transplant 2013, 22:1577-1590. 10.3727/096368912X659907, 23294734.
25. Park JH, Hwang I, Hwang SH, Han H, Ha H. Human umbilical cord blood-derived mesenchymal stem cells prevent diabetic renal injury through paracrine action. Diabetes Res Clin Pract 2012, 98:465-473. 10.1016/j.diabres.2012.09.034, 23026513.
26. Park JH, Park J, Hwang SH, Han H, Ha H. Delayed treatment with human umbilical cord blood-derived stem cells attenuates diabetic renal injury. Transplant Proc 2012, 44:1123-1126. 10.1016/j.transproceed.2012.03.044, 22564642.
27. An JH, Park H, Song JA, Ki KH, Yang JY, Choi HJ, Cho SW, Kim SW, Kim SY, Yoo JJ, Baek WY, Kim JE, Choi SJ, Oh W, Shin CS. Transplantation of human umbilical cord blood-derived mesenchymal stem cells or their conditioned medium prevents bone loss in ovariectomized nude mice. Tissue Eng Part A 2013, 19:685-696. 10.1089/ten.tea.2012.0047, 23215868.
28. Roura S, Bago JR, Soler-Botija C, Pujal JM, Galvez-Monton C, Prat-Vidal C, Llucia-Valldeperas A, Blanco J, Bayes-Genis A. Human umbilical cord blood-derived mesenchymal stem cells promote vascular growth in vivo. PLoS One 2012, 7:e49447. 10.1371/journal.pone.0049447, 3500294, 23166670.
29. Lim JY, Jeong CH, Jun JA, Kim SM, Ryu CH, Hou Y, Oh W, Chang JW, Jeun SS. Therapeutic effects of human umbilical cord blood-derived mesenchymal stem cells after intrathecal administration by lumbar puncture in a rat model of cerebral ischemia. Stem Cell Res Ther 2011, 2:38. 10.1186/scrt79, 3308035, 21939558.
30. Choi MY, Yeo SW, Park KH. Hearing restoration in a deaf animal model with intravenous transplantation of mesenchymal stem cells derived from human umbilical cord blood. Biochem Biophys Res Commun 2012, 427:629-636. 10.1016/j.bbrc.2012.09.111, 23026045.
31. Joyce NC, Harris DL, Markov V, Zhang Z, Saitta B. Potential of human umbilical cord blood mesenchymal stem cells to heal damaged corneal endothelium. Mol Vis 2012, 18:547-564. 3298421, 22419848.
32. Kim JY, Kim DH, Kim JH, Lee D, Jeon HB, Kwon SJ, Kim SM, Yoo YJ, Lee EH, Choi SJ, Seo SW, Lee JI, Na DL, Yang YS, Oh W, Chang JW. Soluble intracellular adhesion molecule-1 secreted by human umbilical cord blood-derived mesenchymal stem cell reduces amyloid-beta plaques. Cell Death Differ 2012, 19:680-691. 10.1038/cdd.2011.140, 3307982, 22015609.
33. Gregoire-Gauthier J, Selleri S, Fontaine F, Dieng MM, Patey N, Despars G, Beausejour CM, Haddad E. Therapeutic efficacy of cord blood-derived mesenchymal stromal cells for the prevention of acute graft-versus-host disease in a xenogenic mouse model. Stem Cells Dev 2012, 21:1616-1626. 10.1089/scd.2011.0413, 21910645.
34. Shi LL, Liu FP, Wang DW. Transplantation of human umbilical cord blood mesenchymal stem cells improves survival rates in a rat model of acute hepatic necrosis. Am J Med Sci 2011, 342:212-217. 10.1097/MAJ.0b013e3182112b90, 21642820.
35. Ngoc PK, Phuc PV, Nhung TH, Thuy DT, Nguyet NT. Improving the efficacy of type 1 diabetes therapy by transplantation of immunoisolated insulin-producing cells. Hum Cell 2011, 24:86-95. 10.1007/s13577-011-0018-z, 21567289.
36. Jung KH, Uhm YK, Lim YJ, Yim SV. Human umbilical cord blood-derived mesenchymal stem cells improve glucose homeostasis in rats with liver cirrhosis. Int J Oncol 2011, 39:137-143.
37. Lv YT, Zhang Y, Liu M, Qiuwaxi JN, Ashwood P, Cho SC, Huan Y, Ge RC, Chen XW, Wang ZJ, Kim BJ, Hu X. Transplantation of human cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells in autism. J Transl Med 2013, 11:196. 10.1186/1479-5876-11-196, 3765833, 23978163.
38. Jin JL, Liu Z, Lu ZJ, Guan DN, Wang C, Chen ZB, Zhang J, Zhang WY, Wu JY, Xu Y. Safety and efficacy of umbilical cord mesenchymal stem cell therapy in hereditary spinocerebellar ataxia. Curr Neurovasc Res 2013, 10:11-20. 10.2174/156720213804805936, 23151076.
39. Li XY, Zheng ZH, Li XY, Guo J, Zhang Y, Li H, Wang YW, Ren J, Wu ZB. Treatment of foot disease in patients with type 2 diabetes mellitus using human umbilical cord blood mesenchymal stem cells: response and correction of immunological anomalies. Curr Pharm Des 2013, 19:4893-4899. 10.2174/13816128113199990326, 23343120.
40. Han H, Chang SK, Chang JJ, Hwang SH, Han SH, Chun BH. Intrathecal injection of human umbilical cord blood-derived mesenchymal stem cells for the treatment of basilar artery dissection: a case report. J Med Case Rep 2011, 5:562. 10.1186/1752-1947-5-562, 3240835, 22136528.
41. Dazey B, Duchez P, Letellier C, Vezon G, Ivanovic Z. Cord blood processing by using a standard manual technique and automated closed system " Sepax" (Kit CS-530). Stem Cells Dev 2005, 14:6-10. 10.1089/scd.2005.14.6, 15725739.
42. Solves P, Planelles D, Mirabet V, Blanquer A, Carbonell-Uberos F. Qualitative and quantitative cell recovery in umbilical cord blood processed by two automated devices in routine cord blood banking: a comparative study. Blood Transfus 2013, 11:405-411. 3729132, 23058859.
43. Aktas M, Buchheiser A, Houben A, Reimann V, Radke T, Jeltsch K, Maier P, Zeller WJ, Kogler G. Good manufacturing practice-grade production of unrestricted somatic stem cell from fresh cord blood. Cytotherapy 2010, 12:338-348. 10.3109/14653241003695034, 20370349.
44. Kocaoemer A, Kern S, Kluter H, Bieback K. Human AB serum and thrombin-activated platelet-rich plasma are suitable alternatives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue. Stem Cells 2007, 25:1270-1278. 10.1634/stemcells.2006-0627, 17255520.
45. Bieback K, Hecker A, Kocaomer A, Lannert H, Schallmoser K, Strunk D, Kluter H. Human alternatives to fetal bovine serum for the expansion of mesenchymal stromal cells from bone marrow. Stem Cells 2009, 27:2331-2341. 10.1002/stem.139, 19544413.
46. Jonsdottir-Buch SM, Lieder R, Sigurjonsson OE. Platelet lysates produced from expired platelet concentrates support growth and osteogenic differentiation of mesenchymal stem cells. PLoS One 2013, 8:e68984. 10.1371/journal.pone.0068984, 3708923, 23874839.
47. Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. ALTEX 2011, 28:305-316.
48. Blande IS, Bassaneze V, Lavini-Ramos C, Fae KC, Kalil J, Miyakawa AA, Schettert IT, Krieger JE. Adipose tissue mesenchymal stem cell expansion in animal serum-free medium supplemented with autologous human platelet lysate. Transfusion 2009, 49:2680-2685.
49. Ding Y, Yang H, Feng JB, Qiu Y, Li DS, Zeng Y. Human umbilical cord-derived MSC culture: the replacement of animal sera with human cord blood plasma. In Vitro Cell Dev Biol Anim 2013, 49(10):771-777. 10.1007/s11626-013-9663-8, 24043577.
50. Shetty P, Bharucha K, Tanavde V. Human umbilical cord blood serum can replace fetal bovine serum in the culture of mesenchymal stem cells. Cell Biol Int 2007, 31:293-298. 10.1016/j.cellbi.2006.11.010, 17208468.
51. Ma HY, Yao L, Yu YQ, Li L, Ma L, Wei WJ, Lu XM, Du LL, Jin YN. An effective and safe supplement for stem cells expansion ex vivo: cord blood serum. Cell Transplant 2012, 21:857-869. 10.3727/096368911X612486, 22182982.
52. Murphy MB, Blashki D, Buchanan RM, Yazdi IK, Ferrari M, Simmons PJ, Tasciotti E. Adult and umbilical cord blood-derived platelet-rich plasma for mesenchymal stem cell proliferation, chemotaxis, and cryo-preservation. Biomaterials 2012, 33:5308-5316. 10.1016/j.biomaterials.2012.04.007, 22542609.
53. Baba K, Yamazaki Y, Ishiguro M, Kumazawa K, Aoyagi K, Ikemoto S, Takeda A, Uchinuma E. Osteogenic potential of human umbilical cord-derived mesenchymal stromal cells cultured with umbilical cord blood-derived fibrin: A preliminary study. J Craniomaxillofac Surg 2013, 41(8):775-782. 10.1016/j.jcms.2013.01.025, 23465638.
54. Escobedo-Lucea C, Bellver C, Gandia C, Sanz-Garcia A, Esteban FJ, Mirabet V, Forte G, Moreno I, Lezameta M, Ayuso-Sacido A, Garcia-Verdugo JM. A xenogeneic-free protocol for isolation and expansion of human adipose stem cells for clinical uses. PLoS One 2013, 8:e67870. 10.1371/journal.pone.0067870, 3706484, 23874459.
55. Fekete N, Rojewski MT, Furst D, Kreja L, Ignatius A, Dausend J, Schrezenmeier H. GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC. PLoS One 2012, 7:e43255. 10.1371/journal.pone.0043255, 3419200, 22905242.
56. Marx RE. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg 2004, 62:489-496. 10.1016/j.joms.2003.12.003, 15085519.
57. Lee JY, Nam H, Park YJ, Lee SJ, Chung CP, Han SB, Lee G. The effects of platelet-rich plasma derived from human umbilical cord blood on the osteogenic differentiation of human dental stem cells. In Vitro Cell Dev Biol Anim 2011, 47:157-164.
58. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop D, Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement. Cytotherapy 2006, 8:315-317. 10.1080/14653240600855905, 16923606.
59. Hua J, Gong J, Meng H, Xu B, Yao L, Qian M, He Z, Zou S, Zhou B, Song Z. Comparison of different methods for the isolation of mesenchymal stem cells from umbilical cord matrix: proliferation and multilineage differentiation as compared to mesenchymal stem cells from umbilical cord blood and bone marrow. Cell Biol Int 2013, [Epub ahead of print].
60. Lee MW, Yang MS, Park JS, Kim HC, Kim YJ, Choi J. Isolation of mesenchymal stem cells from cryopreserved human umbilical cord blood. Int J Hematol 2005, 81:126-130. 10.1532/IJH97.A10404, 15765780.
61. Laitinen A, Laine J. Isolation of mesenchymal stem cells from human cord blood. Curr Protoc Stem Cell Biol 2007, Chapter 2:Unit 2A 3.
62. Prins HJ, Rozemuller H, Vonk-Griffioen S, Verweij VG, Dhert WJ, Slaper-Cortenbach IC, Martens AC. Bone-forming capacity of mesenchymal stromal cells when cultured in the presence of human platelet lysate as substitute for fetal bovine serum. Tissue Eng Part A 2009, 15:3741-3751. 10.1089/ten.tea.2008.0666, 19519274.
63. Jung J, Moon N, Ahn JY, Oh EJ, Kim M, Cho CS, Shin JC, Oh IH. Mesenchymal stromal cells expanded in human allogenic cord blood serum display higher self-renewal and enhanced osteogenic potential. Stem Cells Dev 2009, 18:559-571. 10.1089/scd.2008.0105, 18754716.
64. Vogel JP, Szalay K, Geiger F, Kramer M, Richter W, Kasten P. Platelet-rich plasma improves expansion of human mesenchymal stem cells and retains differentiation capacity and in vivo bone formation in calcium phosphate ceramics. Platelets 2006, 17:462-469. 10.1080/09537100600758867, 17074722.