Recombinant protein expression in Lactococcus lactis using the P170 expression system

FEMS Microbiology Letters

Volumn 351, Issue 2, 2014, Pages 170-178

  Jørgensen, Casper M ^a   Vrang, Astrid ^a   Madsen, Søren M ^a  

^a BIONEER A S   (Denmark)

Author keywords

Fermentation; Optimised production strains; REED technology; Signal peptides

Indexed keywords

LACTIC ACID; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM ISOLATION; BATCH FERMENTATION; DIALYSIS; ELECTRODIALYSIS; ENZYME SYNTHESIS; GENE EXPRESSION SYSTEM; GROWTH INHIBITION; LACTOCOCCUS LACTIS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN SECRETION; REVERSE ELECTRO ENHANCED DIALYSIS; STAPHYLOCOCCUS AUREUS; UPREGULATION;

ANIMALIA; LACTOCOCCUS LACTIS; POSIBACTERIA; STAPHYLOCOCCUS AUREUS;

FERMENTATION; OPTIMISED PRODUCTION STRAINS; REED™ TECHNOLOGY; SIGNAL PEPTIDES;

CULTURE MEDIA; DIALYSIS; FERMENTATION; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; LACTIC ACID; LACTOCOCCUS LACTIS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; TRANSCRIPTIONAL ACTIVATION;

EID: 84896700545     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/1574-6968.12351     Document Type: Article

References (56)

1. Aggerbeck H & Madsen SM (2006) Safety of ESAT-6. Tuberculosis 86: 363-373.
2. Aggerbeck H, Giemza R, Joshi P, Tingskov PN, Hoff ST, Boyle J, Andersen P & Lewis DJ (2013) Randomised clinical trial investigating the specificity of a novel skin test (C-Tb) for diagnosis of M. tuberculosis infection. PLoS One 8: e64215.
3. Bélard S, Issifou S, Hounkpatin AB et al. (2011) A randomized controlled phase Ib trial of the malaria vaccine candidate GMZ2 in African children. PLoS One 6: e22525.
4. Bermúdez-Humarán LG, Kharrat P, Chatel JM & Langella P (2011) Lactococci and lactobacilli as mucosal delivery vectors for therapeutic proteins and DNA vaccines. Microb Cell Fact 10(Suppl 1): S4.
5. Bernaudat F, Frelet-Barrand A, Pochon N et al. (2011) Heterologous expression of membrane proteins: choosing the appropriate host. PLoS One 6: e29191.
6. Bron PA, Benchimol MG, Lambert J, Palumbo E, Deghorain M, Delcour J, De Vos WM, Kleerebezem M & Hols P (2002) Use of the alr gene as a food-grade selection marker in lactic acid bacteria. Appl Environ Microb 68: 5663-5670.
7. Chatzi KE, Sardis MF, Karamanou S & Economou A (2013) Breaking on through to the other side: protein export through the bacterial Sec system. Biochem J 449: 25-37.
8. Cheung QC, Yuan Z, Dyce PW, Wu D, DeLange K & Li J (2009) Generation of epidermal growth factor-expressing Lactococcus lactis and its enhancement on intestinal development and growth of early-weaned mice. Am J Clin Nutr 89: 871-879.
9. Cho MH, Park SE, Lee MH, Ha SJ, Kim HY, Kim MJ, Lee SJ, Madsen SM & Park CS (2007) Extracellular secretion of a maltogenic amylase from Lactobacillus gasseri ATCC33323 in Lactococcus lactis MG1363 and its application on the production of branched maltooligosaccharides. J Microbiol Biotechn 17: 1521-1526.
10. Cocaign-Bousquet M, Garrigues C, Novak L, Lindley ND & Loubiere P (1995) Rational development of a simple synthetic medium for the sustained growth of Lactococcus lactis. J Appl Bacteriol 79: 108-116.
11. Cortes-Perez NG, Poquet I, Oliveira M, Gratadoux JJ, Madsen SM, Miyoshi A, Corthier G, Azevedo V, Langella P & Bermúdez-Humarán LG (2006) Construction and characterization of a Lactococcus lactis strain deficient in intracellular ClpP and extracellular HtrA proteases. Microbiology 152: 2611-2618.
12. Douillard FP, O'Connell-Motherway M, Cambillau C & van Sinderen D (2011) Expanding the molecular toolbox for Lactococcus lactis: construction of an inducible thioredoxin gene fusion expression system. Microb Cell Fact 10: 66-75.
13. Duwat P, Sourice S, Cesselin B, Lamberet G, Vido K, Gaudu P, Le Loir Y, Violet F, Loubière P & Gruss A (2001) Respiration capacity of the fermenting bacterium Lactococcus lactis and its positive effects on growth and survival. J Bacteriol 183: 4509-4516.
14. Esen M, Kremsner PG, Schleucher R et al. (2009) Safety and immunogenicity of GMZ2 - a MSP3-GLURP fusion protein malaria vaccine candidate. Vaccine 27: 6862-6868.
15. Frelet-Barrand A, Boutigny S, Moyet L et al. (2010) Lactococcus lactis, an alternative system for functional expression of peripheral and intrinsic Arabidopsis membrane proteins. PLoS One 5: e8746.
16. Fuhlendorff R, Garde A & Rype JU (2009) Electro membrane technology boosting bioreactor processes. Pharmaceutical Engineering On-line exclusive 29: no. 4.
17. Glenting J, Poulsen LK, Kato K, Madsen SM, Frøkiær H, Wendt C & Sørensen HW (2007) Production of recombinant peanut allergen Ara h 2 using Lactococcus lactis. Microb Cell Fact 6: 28-37.
18. Gu Q, Song D & Zhu M (2009) Oral vaccination of mice against Helicobacter pylori with recombinant Lactococcus lactis expressing urease subunit B. FEMS Immunol Med Microbiol 56: 197-203.
19. Israelsen H & Hansen EB (1993) Insertion of transposon Tn917 derivatives into the Lactococcus lactis subsp. lactis chromosome. Appl Environ Microb 59: 21-26.
20. Israelsen H, Madsen SM, Johansen E, Vrang A & Hansen EB (1995a) Environmentally regulated promoters in Lactococci. Genetics of Streptococci, Enterococci and Lactococci (Ferretti JJ, Gilmore MS, Klaenhammer TR & Brown F, eds). Developments in Biological Standards, 85: 443-448. Karger, Basel.
21. Israelsen H, Madsen SM, Vrang A, Hansen EB & Johansen E (1995b) Cloning and partial characterization of regulated promoters from Lactococcus lactis Tn917-lacZ integrants with the new promoter probe vector, pAK80. Appl Environ Microb 61: 2540-2547.
22. Jensen PR & Hammer K (1993) Minimal requirements for exponential growth of Lactococcus lactis. J Bacteriol 59: 4363-4366.
23. Jepsen MP, Jogand PS, Singh SK et al. (2013) The malaria vaccine candidate GMZ2 elicits functional antibodies in individuals from malaria endemic and non-endemic areas. J Infect Dis 208: 479-488.
24. Jørgensen F, Hansen OC & Stougaard P (2001) High-efficiency synthesis of oligosaccharides with a truncated beta-galactosidase from Bifidobacterium bifidum. Appl Microbiol Biotechnol 57: 647-652.
25. Jørgensen F, Hansen OC & Stougaard P (2004) Enzymatic conversion of D-galactose to D-tagatose: heterologous expression and characterisation of a thermostable L-arabinose isomerase from Thermoanaerobacter mathranii. Appl Microbiol Biotechnol 64: 816-822.
26. Kang P, Toms D, Yin Y, Cheung Q, Gong J, De Lange K & Li J (2010) Epidermal growth factor-expressing Lactococcus lactis enhances intestinal development of early-weaned pigs. J Nutr 140: 806-811.
27. Kuipers OP, Beerthuyzen MM, de Ruyter PG, Luesink EJ & de Vos WM (1995) Autoregulation of nisin biosynthesis in Lactococcus lactis by signal transduction. J Biol Chem 270: 27299-27304.
28. Kunji ER, Slotboom DJ & Poolman B (2003) Lactooccus lactis as host for overproduction of functional membrane proteins. Biochim Biophys Acta 1610: 97-108.
29. Linares DM, Kok J & Poolman B (2010) Genome sequences of Lactococcus lactis MG1363 (revised) and NZ9000 and comparative physiological studies. J Bacteriol 192: 5806-5812.
30. Llull D & Poquet I (2004) New expression system tightly controlled by zinc availability in Lactococcus lactis. Appl Environ Microb 70: 5398-5406.
31. Madsen SM, Arnau J, Vrang A, Givskov M & Israelsen H (1999) Molecular characterization of the pH-inducible and growth phase-dependent promoter P170 of Lactococcus lactis. Mol Microbiol 32: 75-87.
32. Madsen SM, Hindré T, Le Pennec JP, Israelsen H & Dufour A (2005) Two acid-inducible promoters from Lactococcus lactis requires the cis-acting ACiD-box and the transcription regulator RcfB. Mol Microbiol 56: 735-746.
33. Mierau I & Kleerebezem M (2005) 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis. Appl Microbiol Biot 68: 705-717.
34. Mierau I, Leij P, van Swam I, Blommestein B, Floris E, Mond J & Smid EJ (2005) Industrial-scale production and purification of a heterologous protein in Lactococcus lactis using the nisin-controlled gene expression system NICE: the case of lysostaphin. Microb Cell Fact 4: 15-23.
35. Miyoshi A, Poquet I, Azevedo V, Commissaire J, Bermudez-Humaran V, Domakova E, Le Loir Y, Oliveira S-C, Gruss A & Langella P (2002) Controlled production of stable heterologous proteins in Lactococcus lactis. Appl Environ Microb 68: 3141-3146.
36. Mordmüller B, Szywon K, Greutelaers B et al. (2010) Safety and immunogenicity of the malaria vaccine candidate GMZ2 in malaria-exposed, adult individuals from Lambaréné, Gabon. Vaccine 28: 6698-6703.
37. Mu D, Montalbán-López M, Masuda Y & Kuipers OP (2013) Zirex: a novel zinc-regulated expression system for Lactococcus lactis. Appl Environ Microb 79: 4503-4508.
38. Ng DTW & Sarkar CA (2013) Engineering signal peptides for enhanced protein secretion from Lactococcus lactis. Appl Environ Microb 79: 347-356.
39. Nouaille S, Commissaire J, Gratadoux JJ, Ravn P, Bolotin A, Gruus A, Le Loir Y & Langella P (2004) Influence of lipoteichoic acid D-alanylation on protein secretion in Lactococcus lactis as revealed by random mutagenesis. Appl Environ Microb 70: 1600-1607.
40. Nouaille S, Morello E, Cortez-Peres N, Le Loir Y, Commissaire J, Gratadoux JJ, Poumerol E, Gruss A & Langella P (2006) Complementation of the Lactococcus lactis secretion machinery with Bacillus subtilis SecDF improves secretion of staphylococcal nuclease. Appl Environ Microb 72: 2272-2279.
41. O'Sullivan DJ & Klaenhammer TR (1993) High- and low-copy-number Lactococcus shuttle cloning vectors with features for clone screening. Gene 137: 227-231.
42. Park JY, Jung JH, Seo DH, Ha SJ, Yoon JW, Kim YC, Shim JH & Park CS (2010) Microbial production of palatinose through extracellular expression of a sucrose isomerase from Enterobacter sp. FMB-1 in Lactococcus lactis MG1363. Bioresource Technol 101: 8828-8833.
43. Poquet I, Saint V, Seznec E, Simoes N, Bolotin A & Gruss A (2000) HtrA is the unique surface housekeeping protease in Lactococcus lactis and is required for natural protein processing. Mol Microbiol 35: 1042-1051.
44. Rasmussen MA, Madsen SM, Stougaard P & Johnsen MG (2008) Flavobacterium sp. strain 4221 and Pedobacter sp. strain 4236 β-1, 3-glucanases that are active at low temperatures. Appl Environ Microb 74: 7070-7072.
45. Ravn P, Arnau J, Madsen SM, Vrang A & Israelsen H (2000) The development of TnNuc and its use for the isolation of novel secretion signals in Lactococcus lactis. Gene 242: 347-356.
46. Ravn P, Arnau J, Madsen SM, Vrang A & Israelsen H (2003) Optimization of signal peptide SP310 for heterologous protein production in Lactococcus lactis. Microbiology 149: 2193-2201.
47. Schneewind O & Missiakas DM (2012) Protein secretion and surface display in Gram-positive bacteria. Philos Trans R Soc Lond B 367: 1123-1139.
48. Schwab C & Gänzle M (2011) Lactic acid bacteria fermentation of human milk oligosaccharide components, human milk oligosaccharides and galactooligosaccharides. FEMS Microbiol Lett 315: 141-148.
49. Schwab C, Sørensen KI & Gänzle MG (2010) Heterologous expression of glycoside hydrolase family 2 and 42 β-galactosidases of lactic acid bacteria in Lactococcus lactis. Syst Appl Microbiol 33: 300-307.
50. Song D & Gu Q (2009) Surface expression of Helicobacter pylori urease subunit B gene E fragment on Lactococcus lactis by means of the cell wall anchor of Staphylococcus aureus protein A. Biotechnol Lett 31: 985-989.
51. Sriraman K & Jayaraman G (2006) Enhancement of recombinant streptokinase production in Lactococcus lactis by suppression of acid tolerance response. Appl Microbiol Biotechnol 72: 1202-1209.
52. Sriraman K & Jayaraman G (2008) HtrA is essential for efficient secretion of recombinant proteins by Lactococcus lactis. Appl Environ Microb 74: 7442-7446.
53. Steen A, Wiederhold E, Gandhi T, Breitling R & Slotboom DJ (2011) Physiological adaptation of the bacterium Lactococcus lactis in response to the production of human CFTR. Mol Cell Proteomics 10: M000052-MCP200.
54. Tan YP, Giffard PM, Barry DG, Huston WM & Turner MS (2008) Random mutagenesis identifies novel genes involved in the secretion of antimicrobial, cell wall-lytic enzymes by Lactococcus lactis. Appl Environ Microb 74: 7490-7496.
55. Theisen M, Soe S, Brunstedt K, Follmann F, Bredmose L, Israelsen H, Madsen SM & Druilhe P (2004) A Plasmodium falciparum GLURP-MSP3 chimeric protein; expression in Lactococcus lactis, immunogenicity and induction of biologically active antibodies. Vaccine 22: 1188-1198.
56. van Asseldonk M, Rutten G, Oteman M, Siezen RJ, de Vos WM & Simons G (1990) Cloning of usp45, a gene encoding a secreted protein from Lactococcus lactis subsp. lactis MG1363. Gene 95: 155-160.