HtrA is essential for efficient secretion of recombinant proteins by Lactococcus lactis

Applied and Environmental Microbiology

Volumn 74, Issue 23, 2008, Pages 7442-7446

  Sriraman, Kalpana ^a   Jayaraman, Guhan ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY MADRAS   (India)

Author keywords

[No Author keywords available]

Indexed keywords

POLARIZATION;

ACID TOLERANCE RESPONSES; EXTRACELLULAR; LACTOCOCCUS LACTIS; PROTEIN SECRETIONS; RECOMBINANT PROTEINS; SIGNIFICANT REDUCTIONS;

PROTEINS;

PROTEIN HTRA; PROTEINASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

BACTERIUM; MUTATION; PROTEIN; RECOMBINATION; SECRETION;

ARTICLE; BACTERIUM MUTANT; GENE MUTATION; LACTOCOCCUS LACTIS; NONHUMAN; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN SECRETION;

BACTERIAL PROTEINS; GENE DELETION; LACTOCOCCUS LACTIS; PEPTIDE HYDROLASES; PROTEIN TRANSPORT; RECOMBINANT PROTEINS;

LACTOCOCCUS LACTIS;

EID: 57149092803     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00638-08     Document Type: Article

References (18)

1. Bermudez-Humaran, L. G., P. Langella, J. Commissaire, S. Gilbert, Y. Le Loir, R. L'Haridon, and G. Corthier. 2003. Controlled intra- or extracellular production of staphylococcal nuclease and ovine omega interferon in Lactococcus lactis. FEMS Microbiol. Lett. 224:307-313.
2. Bredmose, L., S. M. Madsen, A. Vrang, P. Ravn, M. G. Johnsen, J. Arnau, and H. Israelsen. 2001. Development of a heterologous gene expression system for use in Lactococcus lactis, p. 269-275. In O. W. Merten, D. Mattonovich, C. Lang, G. Larsson, P. Neubauer, D. Porro, P. Postma, J. T. de Mattos, and J. A. Cole (ed.), Recombinant protein production with prokaryotic and eukaryotic cells. Kluwer Academic Publishers, Dordrecht, The Netherlands.
3. Cortes-Perez, N. G., I. Poquet, M. Oliveira, J. J. Gratadoux, S. M. Madsen, A. Miyoshi, G. Corthier, V. Azevedo, P. Langella, and L. G. Bermudez-Humaran. 2006. Construction and characterization of a Lactococcus lactis strain deficient in intracellular ClpP and extracellular HtrA proteases. Microbiology 152:2611-2618.
4. Erickson, A., and R. H. Deibel. 1973. Turbidimetric assay of staphylococcal nuclease. Appl. Microbiol. 25:337-341.
5. Foucaud-Scheunemann, C., and I. Poquet. 2003. HtrA is a key factor in the response to specific stress conditions in Lactococcus lactis. FEMS Microbiol. Lett. 224:53-59.
6. Gasson, M. J. 1983. Plasmid complements of Streptococcus lactis NCDO712 and other lactis streptococci after protoplast-induced curing. J. Bacteriol. 154:1-9.
7. Israelsen, H., S. M. Madsen, A. Vrang, E. B. Hansen, and E. Johansen. 1995. Cloning and partial characterization of regulated promoters from Lactococcus lactis Tn917-lacZ integrants with the new promoter probe vector, pAK80. Appl. Environ. Microbiol. 61:2540-2547.
8. Kuipers, O. P., P. G. G. A. de Ruyter, M. Kleerebezem, and W. M. de Vos. 1998. Quorum sensing controlled gene expression in lactic acid bacteria. J. Biotechnol. 64:15-21.
9. Madsen, S. M., J. Arnau, A. Vrang, M. Givskov, and H. Israelsen. 1999. Molecular characterization of the pH-inducible and growth phase-dependent promoter P170 of Lactococcus lactis. Mol. Microbiol. 32:75-87.
10. Madsen, S. M., T. Hindre, J. Le Pennec, H. Israelsen, and A. Dufour. 2005. Two acid-inducible promoters from Lactococcus lactis require the cis-acting ACiD box and the transcription regulator RcfB. Mol. Microbiol. 56:735-746.
11. Mierau, I., and M. Kleerebezem. 2005. 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis. Appl. Microbiol. Biotechnol. 68:705-717.
12. Miyoshi, A., I. Poquet, V. Azevedo, J. Commissaire, L. Bermudez-Humaran, E. Domakova, Y. Le Loir, S. C. Oliveira, A. Gruss, and P. Langella. 2002. Controlled production of stable heterologous proteins in Lactococcus lactis. Appl. Environ. Microbiol. 68:3141-3146.
13. Morello, E., L. G. Bermudez-Humaran, D. Llull, V. Sole, N. Miraglio, P. Langella, and I. Poquet. 2008. Lactococcus lactis, an efficient cell factory for recombinant protein production and secretion. J. Mol. Microbiol. Biotechnol. 14:48-58.
14. Obis, D., A. Guillot, J. C. Gripon, P. Renault, A. Bolotin, and M. Y. Mistou. 1999. Genetic and biochemical characterization of a high-affinity betaine uptake system in Lactococcus lactis reveals a new functional organization within bacterial ABC transporters. J. Bacteriol. 181:6238-6246.
15. Poquet, I., V. Saint, E. Seznec, N. Simoes, N. Bolontin, and A. Gruss. 2000. HtrA is the unique surface housekeeping protease in Lactococcus lactis and is required for natural protein processing. Mol. Microbiol. 35:1042-1051.
16. Rigoulay, C., I. Poquet, S. M. Madsen, and A. Gruss. 2004. Expression of Staphylococcus aureus surface proteins HtrA1 and HtrA2 in Lactococcus lactis. FEMS Microbiol. Lett. 237:279-288.
17. Rozen, S., and H. J. Skaletsky. 2000. Primer3 on the WWW for general users and for biologist programmers, p. 365-386. In S. Krawetz and S. Misener (ed.), Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJ.
18. Sriraman, K., and G. Jayaraman. 2006. Enhancement of recombinant streptokinase production in Lactococcus lactis by suppression of acid tolerance response. Appl. Microbiol. Biotechnol. 72:1202-1209.