Enzymatic detachment of therapeutic mesenchymal stromal cells grown on glass carriers in a bioreactor

Open Biomedical Engineering Journal

Volumn 7, Issue 1, 2013, Pages 147-158

  Salzig, Denise ^a   Schmiermund, Alexandra ^a   Grace, Pablo P ^a   Elseberg, Christiane ^a   Weber, Christian ^a   Czermak, Peter ^a,b,c  

^a TECHNISCHE HOCHSCHULE MITTELHESSEN   (Germany)

^b JUSTUS LIEBIG UNIVERSITY   (Germany)

^c Kansas State University   (United States)

Author keywords

Atmp; Bioreactor; Enzymatic detachment; Glass carrier; Hmsc

Indexed keywords

BIOREACTORS; CELL CULTURE; ENCAPSULATION; GLASS;

ATMP; COMMERCIAL AVAILABILITY; CULTURE ENVIRONMENTS; ENZYMATIC DETACHMENT; EXPERIMENTAL CONDITIONS; GLASS CARRIER; HMSC; MESENCHYMAL STROMAL CELLS;

CELLS;

GUANOSINE PHOSPHATE; NITRILOTRI(METHYLENEPHOSPHONIC ACID); PHOSPHONIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; BIOREACTOR EQUIPMENT; CELL ADHESION; CELL CULTURE; CELL DAMAGE; CELL DETACHMENT; CELL ENCAPSULATION; CELL FUNCTION; CELL HARVEST; CELL KINETICS; CELL THERAPY; ENZYMATIC ASSAY; ENZYMATIC DETACHMENT; ENZYME ACTIVITY; HUMAN; HUMAN CELL; IN VITRO STUDY; MESENCHYMAL STROMA CELL; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS;

EID: 84896693148     PISSN: None     EISSN: 18741207     Source Type: Journal    
DOI: 10.2174/1874120701307010147     Document Type: Article

References (34)

1. H. Hamada, M. Kobune, K. Nakamura, Y. Kawano, K. Kato, O. Honmou, K. Houkin, T. Matsunaga, and Y. Niitsu, Mesenchymal stem cells (MSC) as therapeutic cytoreagents for gene therapy, Can. Sci., vol. 96, pp. 149-156, 2005.
2. D. Freimark, P. Pino-Grace, S. Pohl, C. Weber, C. Wallrapp, P. Geigle, R. Pörtner, and P. Czermak, Use of encapsulated stem cells to overcome the bottleneck of cell availability for cell therapy approaches, Transfusion Med. Hemoth., vol. 37, pp. 66-73, 2010.
3. K. Cierpka, C. L. Elseberg, K. Niss, M. Kassem, D. Salzig, and P. Czermak, hMSC Production in Disposable Bioreactors with Regards to GMP and PAT, Chemie Ingenieur Technik, vol. 85, pp. 67-75, 2013.
4. C. Weber, S. Pohl, R. Poertner, P. Pino-Grace, D. Freimark, C. Wallrapp, P. Geigle, and P. Czermak, Production process for stem cell-based therapeutic implants - Expansion of the production cell line and cultivation of encapsulated cells, In: Advances in Biochemical Engineering/Biotechnology. C. Kasper, M. van Griensven, and R. Poertner, Eds., Berlin: Springer, pp. 143-162, 2010.
5. C. Weber, S. Pohl, R. Portner, C. Wallrapp, M. Kassem, P. Geigle, and P. Czermak, Expansion and Harvesting of hMSC-TERT, Open Biomed. Eng. J., vol. 1, pp. 38-46, 2007.
6. C. Weber, S. Pohl, P. Pino Grace, D. Freimark, C. Wallrapp, P. Geigle, and P. Czermak, Expansion of human mesenchymal stem cells in a fixed-bed bioreactor system - Part A: Inoculation, cultivation, cell harvest procedures, Int. J. Artif Organs, vol. 33, pp. 512-525, 2010.
7. C. Weber, S. Pohl, R. Poertner, P. Pino Grace, D. Freimark, C. Wallrapp, P. Geigle, and P. Czermak, Expansion of human mesenchymal stem cells in a fixed-bed bioreactor system - Part B: Modeling and scale up, Int. J. Artif Organs, vol. 33, pp. 782-795, 2010.
8. C. L. Elseberg, J. Leber, D. Salzig, C. Wallrapp, M. Kassem, M. Kraume, and P. Czermak, Microcarrier-based expansion process for hMSCs with high vitality and undifferentiated characteristics, Int. J. Artif Organs, vol. 35, pp. 93-107, Feb 2012.
9. O.-W. Merten and M. C. Flickinger, Cell Detachment, in Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation and Cell Technology, John Wiley & Sons, Inc., pp. 1-22, 2009.
10. FDA, FDA Proposes Barring Ceratin Cattle Material From Medical Products As BSE Safeguard, 2007.
11. H. E. Canavan, X. Cheng, D. J. Graham, B. D. Ratner, and D. G. Castner, Cell sheet detachment affects the extracellular matrix: A surface science study comparing thermal liftoff, enzymatic, and mechanical methods, J. Biomed. Mat. Res. Part A, vol. 75A, pp. 1-13, 2005.
12. F-P. Wachs, S. Couillard-Despres, M. Engelhardt, D. Wilhelm, S. Ploetz, M. Vroemen, J. Kaesbauer, G. Uyanik, J. Klucken, C. Karl, J. Tebbing, C. Svendsen, N. Weidner, H.-G. Kuhn, J. Winkler, and L. Aigner, High Efficacy of Clonal Growth and Expansion of Adult Neural Stem Cells, Lab. Invest., vol. 83, pp. 949-962, 2003.
13. R. Bajpai, J. Lesperance, M. Kim, and A. V. Terskikh, Efficient propagation of single cells accutase-dissociated human embryonic stem cells, Mol. Reprod. Develop., vol. 75, pp. 818-827, 2008.
14. M. Vielreicher, G. Harms, E. Butt, U. Walter, and A. Obergfell, Dynamic interaction between Src and C-terminal Src kinase in integrin alphaIIbbeta3-mediated signaling to the cytoskeleton, J. Biol. Chem., vol. 282, pp. 33623-33631, 2007.
15. I. Grimm, S. N. Ullsperger, and H. Zimmermann, Nucleotides and epidermal growth factor induce parallel cytoskeletal rearrange-ments and migration in cultured adult murine neural stem cells, Acta Physiol, vol. 199, pp. 181-189.
16. E. Lasfargues and D. Moore, A method for the continuous cultivation of mammary epithelium, In Vitro Cell Develop. Bio. - Plant, vol. 7, pp. 21-25, 1971.
17. E. Sjögren-Jansson, M. Zetterström, K. Moya, J. Lindqvist, R. Strehl, and P. S. Eriksson, Large-scale propagation of four undifferentiated human embryonic stem cell lines in a feeder-free culture system, Develop. Dyn., vol. 233, pp. 1304-1314, 2005.
18. S. Rourou, A. v. d. Ark, T. v. d. Velden, and H. l. Kallel, Development of an Animal-Component Free Medium for Vero Cells Culture, Biotechnol. Prog., vol. 25, pp. 1752-1761, 2009.
19. J. M. Wiehe, Z. Kaya, J. M. Homann, J. Wöhrle, K. Vogt, T. Nguyen, W. Rottbauer, J. Torzewski, N. Fekete, M. Rojewski, H. Schrezenmeier, B. Moepps, and O. Zimmermann, GMP-adapted overexpression of CXCR4 in human mesenchymal stem cells for cardiac repair, Int. J. Cardiol., vol. available online, 2012.
20. J. L. Simonsen, C. Rosada, N. Serakinci, J. Justesen, K. Stenderup, S. I. S. Rattan, T. G. Jensen, and M. Kassem, Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells, Nat. Biotech., vol. 20, pp. 592-596, 2002.
21. M. Butler, Animal Cell Culture and Technology - The Basics. New York: Oxford University Press, 2004.
22. C. Justice, J. Leber, D. Freimark, P. Pino Grace, M. Kraume, and P. Czermak, Online- and offline- monitoring of stem cell expansion on microcarrier, Cytotechnology, pp. 1-11, 2011.
23. J. Teichmann, M. Valtink, S. Gramm, M. Nitschke, C. Werner, R. H. W. Funk, and K. Engelmann, Human corneal endothelial cell sheets for transplantation: Thermo-responsive cell culture carriers to meet cell-specific requirements, Acta Biomater, vol. 9, pp. 5031-5039, 2013.
24. C. Elseberg, P. Grace, D. Salzig, and P. Czermak, Device and for expansion and harvest of adherent cells, EP12000816.6 2012.
25. E. Zini, M. Franchini, F. Guscetti, M. Osto, K. Kaufmann, M. Ackermann, T. A. Lutz, and C. E. Reusch, Assessment of six different collagenase-based methods to isolate feline pancreatic islets, Res. Vet. Sci., vol. 87, pp. 367-372, 2009.
26. K. S. Stenn, R. Link, G. Moellmann, J. Madri, and E. Kuklinska, Dispase, a neutral protease from Bacillus polymyxa, is a powerful fibronectinase and type IV collagenase, J. Investig. Dermatol., vol. 93, pp. 287-90, 1989.
27. J. M. Harlan, P. D. Killen, L. A. Harker, G. E. Striker, and D. G. Wright, Neutrophil-mediated Endothelial Injury In Vitro, J. Clin. Invest., vol. 68, p. 10, 1981.
28. C. M. Flickinger and W. S. Drew, Encyclopedia of bioprocess technology: Fermentation, Biocatalysis and Bioseperation, John Wiley & Sons, Inc, vol. 1-5, 1999.
29. C. Waymouth, To disaggregate or not to disaggregate, injury and cell disaggregation, transient or permanent?, In Vitro Cell Develop. Biol. - Plant, vol. 10, pp. 97-111, 1974.
30. J. M. Berg, J. L. Tymoczko, and L. Stryer, Stryer Biochemie, Spektrum Akademischer Verlag, 2007.
31. Z. Wu, G. Jiang, P. Xiang, D. Yang, and N. Wang, Purification and characterization of trypsin-like enzymes from North Pacific krill., Biotechnol. Lett., vol. 30, pp. 67-72, 2008.
32. C. Ellerström, R. Strehl, K. Noaksson, J. Hyllner, and H. Semb, Facilitated Expansion of Human Embryonic Stem Cells by Single-Cell Enzymatic Dissociation, Stem Cells, vol. 25, pp. 1690-1696, 2007.
33. J. Litwin, Standardization of Human Diploid Fibroblast Cultivation: Trypsinization Procedure, Appl. Microbiol., vol. 21, pp. 169-174, 1971.
34. J. Seidel, G. E., M. L. Turk, P. W. Gordy, and R. A. Bowen, 240 Recombinant bovine trypsin made in maize inactivates bovine herpes virus-1 adsorbed to the bovine zona pellucida Reprod., Fertil Develop., vol. 19, pp. 236-236, 2006.