Expansion of human mesenchymal stem cells in a fixed-bed bioreactor system based on non-porous glass carrier - Part B: Modeling and scale-up of the system

International Journal of Artificial Organs

Volumn 33, Issue 11, 2010, Pages 782-795

  Weber, Christian ^a   Freimark, Denise ^a   Pörtner, Ralf ^b   Pino Grace, Pablo ^a   Pohl, Sebastian ^a   Wallrapp, Christine ^c   Geigle, Peter ^c   Czermak, Peter ^a,d  

^a TECHNISCHE HOCHSCHULE MITTELHESSEN   (Germany)

^b HAMBURG UNIVERSITY OF TECHNOLOGY   (Germany)

^c CellMed AG   (Germany)

^d Kansas State University   (United States)

Author keywords

Fixed bed bioreactor; Glass carrier; hMSC TERT; Mesenchymal stem cells; Scale up

Indexed keywords

BIOCONVERSION; BOROSILICATE GLASS; CELL CULTURE; STEM CELLS;

BIOREACTOR SYSTEM; CELL-BE; CELL/B.E; FIXED-BED BIOREACTOR; GLASS CARRIER; HUMAN MESENCHYMAL STEM CELL-TERT; HUMAN MESENCHYMAL STEM CELLS; MESENCHYMAL STEM CELL; POROUS GLASS; SCALE-UP;

BIOREACTORS;

ALGINIC ACID; BOROSILICATE; GLASS; GLUCOSE; SILICATE; UNCLASSIFIED DRUG;

ARTICLE; CELL COUNT; CELL CULTURE; CELL EXPANSION; CELL GROWTH; CONCENTRATION (PARAMETERS); ENCAPSULATION; EXPERIMENTAL STUDY; FILTER REACTOR; IMPLANT; LABORATORY TEST; MESENCHYMAL STEM CELL; OXYGEN SATURATION; SCALE UP; SIMULATION; STROKE;

EID: 78650801253     PISSN: 03913988     EISSN: None     Source Type: Journal    
DOI: 10.1177/039139881003301103     Document Type: Article

References (37)

1. Heile AM, Wallrapp C, Klinge PM, et al. Cerebral Q1 transplantation of encapsulated mesenchymal stem cells improves cellular pathology after experimental traumatic brain injury. Neurosci Lett 2009; 463: 176-81.
2. Vijaa L, Fargec D, Gautierb JF, et al. Mesenchymal stem cells: Stem cell therapy perspectives for type 1 diabetes. Diabetes Metab 2009; 35: 85-93.
3. Weber C, Freimark D, Poertner R, Pino-Grace P, Pohl S, Wallrapp C, Geigle P, Czermak P. Expansion of human mesenchymal stem cells in a fixed-bed bioreactors system - Part A: Inoculation, cultivation, and harvesting procedures. Int J Artif Organs 2010; 33; 512-25.
4. Weber C, Pohl S, Poertner R, et al. Production process for stem cell-based therapeutic implants - Expansion of the production cell line and cultivation of encapsulated cells. In: Kasper C, van Griensven M, Poertner R, eds. Advances in Biochemical Engineering/Biotechnology. Berlin: Springer; 2010 (in press).
5. Simonsen JL, Rosada C, Serakinci N, et al. Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells. Nat Biotechnol 2002; 20: 592-6.
6. Weber C, Pohl S, Pörtner R, et al. Cultivation and differentiation of encapsulated hMSC-TERT in a disposable smallscale syringe-like fixed bed reactor. Open Biomed Eng J 2007;1: 64-70.
7. Higuera G, Schop D, Janssen F, van Dijkhuizen-Radersma R, van Boxtel T, van Blitterswijk CA. Quantifying in vitro growth and metabolism kinetics of human mesenchymal stem cells using a mathematical model. Tissue Eng Part A 2009; 15: 2653-63.
8. Karabelas AJ, Wegner TH, Hanratty TJ. Use of asymptotic relations to correlate mass transfer data in packed beds. Chem Eng Sci 1971; 26: 1581-9.
9. Hung SC, Pochampally RR, Hsu SC, et al. Short-term exposure of multipotent stromal cells to low oxygen increases their expression of CX3CR1 and CXCR4 and their engraftment in vivo. PLoS ONE 2007; 2: e416.
10. Fehrer C, Brunauer R, Laschober G, Unterluggauer H, Reitinger S, Kloss F. Reduced oxygen tension attenuates differentiation capacity of human mesenchymal stem cells and prolongs their lifespan. Aging Cell 2007; 6: 745-57.
11. Krinner A, Zscharnack M, Bader A, Drasdo D, Galle J. Impact of oxygen environment on mesenchymal stem cell expansion and chondrogenic differentiation. Cell Prolif 2009; 42: 471-84.
12. Grayson WL, Zhao F, Izadpanah R, Bunnell B, Ma T. Effects of hypoxia on human mesenchymal stem cell expansion and plasticity in 3D constructs. J Cell Physiol 2006; 207: 331-9.
13. Ma T, Grayson WL, Fröhlich M, Vunjak-Novakovic G. Hypoxia and stem cell-based engineering of mesenchymal tissues. Biotechnol Prog 2009; 25: 32-42.
14. Weber C, Gokorsch S, Czermak P. Expansion and chondrogenic differentiation of human mesenchymal stem cells. Int J Artif Organs 2007; 30: 611-8. (Pubitemid 47455591)
15. Weber C, Pohl S, Pörtner R, et al. Expansion and harvesting of hMSC-TERT. Open Biomed Eng J 2007; 1: 38-46.
16. Pörtner R, Koop M. A model for oxygen supply in fixed bed reactors with immobilized hybridoma cells. Bioprocess Biosyst Eng 1997; 17: 269-75.
17. Venâncio A, Teixeira JA. Characterization of sugar diffusion coefficients in alginate membranes. Biotechnol Tech 1997; 11: 183-6.
18. Weast RC. CRC handbook of chemistry and physics. Clermont, Florida: CRC Press, 1978.
19. Croughan MS, Sayre ES, Wang DI. Viscous reduction of turbulent damage in animal cell culture. Biotechnol Bioeng 1988; 33: 862-72.
20. Lonergan T, Brenner C, Bavister B. Differentiation-related changes in mitochondrial properties as indicators of stem cell competence. J Cell Physiol 2006; 208: 149-53.
21. Conget PA, Minguell JJ. Phenotypical and functional properties of human bone marrow mesenchymal progenitor cells. J Cell Physiol 1999; 181: 67-73. (Pubitemid 29400373)
22. Guo Z, Yang J, Liu X, et al. Biological features of mesenchymal stem cells from human bone marrow. Chin Med J (Engl) 2001; 114: 950-3.
23. Liu Y-H, Bi J-X, Zeng A-P, yuan J-Q. A simple kinetic model for myeloma cell culture with consideration of lysine limitation. Bioprocess Biosyst Eng 2008; 31: 569-77.
24. Soukup T, Mokrý J, Karbanová J, Pytlík R, Suchomel P, Kučerová L. Mesenchymal stem cells isolated from human bone marrow: cultivation, phenotypic analysis and changes in proliferation kinetics. Acta Med (Hradec Kralove) 2006; 49: 27-33.
25. Amit M, Carpenter MK, Inokuma MS, Chiu CP, Thomson JA. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of pulture. Dev Biol 2000; 227: 271-8.
26. Abranches E, Bekman E, Henrique D, Cabral JM. Expansion of mouse embryonic stem cells on microcarriers. Biotechnol Bioeng 2006; 96: 1211-21.
27. Breguet V, Gugerli RI, von Stockar U, Marison IW. CHO immobilization in alginate/poly-L-lysine microcapsules: an understanding of potential and limitations. Cytotechnology 2007; 53: 81-93.
28. Shirai Y, Hashimoto K, Takamatsu H. Growth kinetics of Hybridoma cells in high density culture. J Ferment Bioeng 1992; 73: 159-65.
29. Schop D, Janssen FW, Borgart E, de Bruijn JD, van Dijkhuizen- Radersma R. Expansion of mesenchymal stem cells using a microcarrier-based cultivation system: growth and metabolism. J Tissue Eng Regen Med 2008; 2: 126-35.
30. Boren J, Cascante M, Marin S, et al. Gleevec (STI571) influences metabolic enzyme activities and glucose carbon flow toward nucleic acid and fatty acid synthesis in myeloid tumor cells. J Biol Chem 2001; 276: 37747-53.
31. Frahm B, Lane P, Atzert H, et al. Adaptive, model-based control by the open-loop-feedback-optimal (OLFO) controller for the effective fed-batch cultivation of hybridoma cells. Biotechnol Prog 2002; 18: 1095-103.
32. Lubiniecki AS. Large-scale mammalian cell culture technology. New York, NY: Marcel Dekker; 1990.
33. Peng CA, Palson BA. Determination of specific oxygen uptake rates in human hematopoietic cultures and implications for bioreactor design. Ann Biomed Eng 1996; 24: 373-81.
34. Acevedo CA, Weinstein-Oppenheimer C, Brown DI, Huebner H, Buchholz R, young ME. A mathematical model for the design of fibrin microcapsules with skin cells. Bioprocess Biosyst Eng 2008; 32: 341-51.
35. De Leòn A, Mayani H, Ramirez OT. Design, characterization and application of a minibioreactor for the culture of human hematopoietic cells under controlled conditions. Cytotechnology 1998; 28: 127-38.
36. Youn BS, Sen A, Behie LA. Scale-up of breast cancer stem cell aggregate cultures to suspension bioreactors. Biotechnol Prog 2006; 22: 801-10. (Pubitemid 43877448)
37. Shirai Y, Hashimoto K, Yamaji H, Kawahara H. Oxygen uptake rate of immobilized growing hybridoma cells. Appl Microbiol Biotechnol 1988; 29: 113-118.