Cell sheet detachment affects the extracellular matrix: A surface science study comparing thermal liftoff, enzymatic, and mechanical methods

Journal of Biomedical Materials Research - Part A

Volumn 75, Issue 1, 2005, Pages 1-13

  Canavan, Heather E ^a,b   Cheng, Xuanhong ^a,b   Graham, Daniel J ^a,b   Ratner, Buddy D ^a,b,c   Castner, David G ^a,b,c  

^a UNIVERSITY OF WASHINGTON   (United States)

^b University of Washington   (United States)

^c University of Washington   (United States)

Author keywords

Cell removal; ECM; N isopropyl acrylamide; ToF SIMS; XPS

Indexed keywords

ADHESION; AMINO ACIDS; CELLS; MATRIX ALGEBRA; PRINCIPAL COMPONENT ANALYSIS; SECONDARY ION MASS SPECTROMETRY; X RAY PHOTOELECTRON SPECTROSCOPY;

CELL REMOVAL; ENZYMATIC DIGESTION; EXTRACELLULAR MATRIX (ECM); TOF-SIMS;

BIOMEDICAL ENGINEERING;

AMINO ACID; POLY(N ISOPROPYLACRYLAMIDE); POLYMER;

ANIMAL CELL; AORTA; ARTICLE; BOVIDS; CELL ADHESION; DEGRADATION; DISSOCIATION; ENDOTHELIUM CELL; EXTRACELLULAR MATRIX; IMMUNOASSAY; IMMUNOHISTOCHEMISTRY; LOW TEMPERATURE; MASS SPECTROMETRY; MONOLAYER CULTURE; NONHUMAN; PRINCIPAL COMPONENT ANALYSIS; X RAY PHOTOELECTRON SPECTROSCOPY;

ACRYLAMIDES; ANIMALS; AORTA; BIOCOMPATIBLE MATERIALS; BIOPHYSICS; CATTLE; CELL ADHESION; CELLS, CULTURED; ENDOTHELIAL CELLS; ENZYMES; EXTRACELLULAR MATRIX; IMMUNOASSAY; POLYMERS; PRINCIPAL COMPONENT ANALYSIS; SPECTROMETRY, X-RAY EMISSION; STRESS, MECHANICAL; SURFACE PROPERTIES; TEMPERATURE; TIME FACTORS;

EID: 24744466948     PISSN: 00219304     EISSN: None     Source Type: Journal    
DOI: 10.1002/jbm.a.30297     Document Type: Article

References (61)

1. Chelobanov BP, Laktionov PP, Khar'kova MV, Rykova EY, Pyshnyi DV, Pyshnaya IA, Silnikov VN, Vlassov VV. Investigation of surface oligonucleotide-binding proteins of eucaryotic cells by affinity modification with reactive oligonucleotide derivatives. Russ Chem Bull 2002;51:1204-1211.
2. Voegele TJ, Voegele-Kadletz M, Esposito V, Macfelda K, Oberndorfer U, Vecsei V, Schabus R. The effect of different isolation techniques on human osteoblast-like cell growth. Anticancer Res 2000;20:3575-3581.
3. Hybbinette S, Bostrom M, Lindberg K. Enzymatic dissociation of keratinocytes from human skin biopsies for in vitro cell propagation. Exp Dermatol 1999;8:30-38.
4. Peakman M, McNab GL, Heaton ND, Tan KC, Vergani D. Development of techniques for obtaining monodispersed human islet cells. Transplantation 1994;57:384-393.
5. Bayad J, Sabolovic N, Bagrel D, Magdalou J, Siest G. Influence of the isolation method on the stability of differentiated phenotype in cultured rat hepatocytes. J Pharmacol Methods 1991; 25:85-94.
6. Vielkind U, Crawford BJ. Evaluation of different procedures for the dissociation of retinal pigmented epithelium into single viable cells. Pigment Cell Res 1988;1:419-433.
7. Tokiwa T, Hoshika T, Shiraishi M, Sato J. Mechanism of cell-dissociation with trypsin and edta. Acta Med Okayama 1979; 33:1-4.
8. Anghileri LJ, Dermietzel. Cell coat in tumor-cells-effects of trypsin and edta: biochemical and morphological study. Oncology 1976;33:17-23.
9. Valkina ES, Kiseleva NS. Possible effect of some dispersing agents (trypsin, edta) on cell composition of zajdelas hepatoma. Bull Exp Biol Med 1974;78:1313-1316.
10. Fujioka N, Morimoto Y, Takeuchi K, Yoshioka M, Kikuchi M. Difference in infrared spectra from cultured cells dependent on cell-harvesting method. Appl Spectrosc 2003;57:241-243.
11. Jung K, Hampel G, Scholz M, Henke W. Culture of human kidney proximal tubular cells-the effect of various detachment procedures on viability and degree of cell detachment. Cell Physiol Biochem 1995;5:353-360.
12. Gospodarowicz D, Ill C. Extracellular-matrix and control of proliferation of vascular endothelial-cells. J Clin Invest 1980; 65:1351-1364.
13. Gospodarowicz D, Greenburg G, Birdwell CR. Determination of cellular shape by extracellular matrix and its correlation with control of cellular growth. Cancer Res 1978;38:4155-4171.
14. Okano T, Yamada N, Sakai H, Sakurai Y. A novel recovery system for cultured cells using plasma-treated polystyrene dishes grafted with poly(N-isopropyl acrylamide). J. Biomed Mater Res 1993:1243-1251.
15. Cheng XH, Wang YB, Hanein Y, Bohringer KF, Ratner BD. Novel cell patterning using microheater-controlled thermoresponsive plasma films. J Biomed Mater Res Part A 2004;70A: 159-168.
16. Okano T, Yamada N, Okuhara M, Sakai H, Sakurai Y. Mechanism of cell detachment from temperature-modulated, hydrophilic-hydrophobic polymer surfaces. Biomaterials 1995:297-303.
17. Doorty KB, Golubeva TA, Gorelov AV, Rochev YA, Allen LT, Dawson KA, Gallagher WM, Keenan AK. Poly(N-isopropyl acrylamide) co-polymer films as potential vehicles for delivery of an antimitotic agent to vascular smooth muscle cells. Cardiovasc Pathol 2003;12:105-110.
18. Shiroyanagi Y, Yamato M, Yamazaki Y, Toma H, Okano T. Transplantable urothelial cell sheets harvested noninvasively from temperature-responsive culture surfaces by reducing temperature. Tissue Eng 2003;9:1005-1012.
19. Nandkumar MA, Yamato M, Kushida A, Konno C, Hirose M, Kikuchi A, Okano T. Two-dimensional cell sheet manipulation of heterotypically co-cultured lung cells utilizing temperature-responsive culture dishes results in long-term maintenance of differentiated epithelial cell functions. Biomaterials 2002;23: 1121-1130.
20. Taillefer J, Brasseur N, van Lier JE, Lenaerts V, Le Garrec D, Leroux JC. In-vitro and in-vivo evaluation of pH-responsive polymeric micelles in a photodynamic cancer therapy model. J Pharm Pharmacol 2001;53:155-166.
21. von Recum HA, Kim SW, Kikuchi A, Okuhara M, Sakurai Y, Okano T. Novel thermally reversible hydrogel as detachable cell culture substrate. J Biomed Mater Res 1998;40:631-639.
22. Zhuang YF, Yang H, Wang GW, Zhu ZQ, Song WQ, Zha HD. Radiation polymerization and controlled drug release of polymer hydrogels with NIPA and NVP. J Appl Polym Sci 2003; 88:724-729.
23. Chen GP, Imanishi Y, Ito Y. Effect of protein and cell behavior on pattern-grafted thermoresponsive polymer. J Biomed Mater Res 1998;42:38-44.
24. Ista LK, Lopez GP. Lower critical solubility temperature Materials as biofouling release agents. J Ind Microbiol Biot 1998; 20:121-125.
25. Bohanon T, Elender G, Knoll W, Koberle P, Lee JS, Offenhausser A, Ringsdorf H, Sackmann E, Simon J, Tovar G, et al. Neural cell pattern formation on glass and oxidized silicon surfaces modified with poly(N-isopropyl acrylamide). J Biomater Sci Polym Ed 1996;8:19-39.
26. Malmstadt N, Yager P, Hoffman AS, Stayton PS. A smart microfluidic affinity chromatography matrix composed of poly(N-isopropyl acrylamide)-coated beads. Anal Chem 2003; 75:2943-2949.
27. Shimizu T, Yamato M, Kikuchi A, Okano T. Cell sheet engineering for myocardial tissue reconstruction. Biomaterials 2003;24:2309-2316.
28. Canavan HE, Cheng X, Ratner BD, Castner DG. Surface characterization of the extracellular matrix remaining after cell detachment from a thermoresponsive polymer. Langmuir 2005;21(5):1949-1955.
29. Fujishige S, Kubota K, Ando I. Phase transition of aqueous solutions of poly(N-isopropyl acrylamide) and poly(N-isopropylmethacrylamide). J Phys Chem 1989;93:3311-3313.
30. Hirotsu S. Critical points of the volume phase transition in N-isopropyl acrylamide gels. J Chem Phys 1988;88:427-431.
31. Otake K, Inomata H, Konno M, Saito S. Thermal analysis of the volume phase transition with N-isopropyl acrylamide gels. Macromolecules 1989;23:283-289.
32. Park TG, Hoffman AS. Synthesis and characterization of Ph- and or temperature-sensitive hydrogels. J Appl Polym Sci 1992; 46:659-671.
33. Kwon OH, Kikuchi A, Yamato M, Okano T. Accelerated cell sheet recovery by co-grafting of PEG with PIPAAm onto porous cell culture membranes. Biomaterials 2003;24:1223-1232.
34. Ito Y, Chen GP, Guan YQ, Imanishi Y. Patterned immobilization of thermoresponsive polymer. Langmuir 1997;13:2756-2759.
35. Ista LK, Mendez S, Perez-Luna VH, Lopez GP. Synthesis of poly(N-isopropyl acrylamide) on initiator-modified self-assembled monolayers. Langmuir 2001;17:2552-2555.
36. Pan YV, Wesley RA, Luginbuhl R, Denton DD, Ratner BD. Plasma polymerized N-isopropyl acrylamide: synthesis and characterization of a smart thermally responsive coating. Biomacromolecules 2001;2:32-36.
37. Gospodarowitz D, Vlodavsky I, Savion N. The extracellular matrix and the control of proliferation of vascular endothelial and vascular smooth muscle cells. J Supramol Str Cell 1980;13: 339-372.
38. Wagner MS, Castner DG. Characterization of adsorbed protein films by time-of-flight secondary ion mass spectrometry with principal component analysis. Langmuir 2001;17:4649-4660.
39. Jackson JE. Principal components and factor-analysis. 1. Principle components. J Qual Technol 1980;12:201-213.
40. Wold S, Esbensen K, Geladi P. Principle component analysis. Chemometrics Intelligent Lab 1987;2:37-52.
41. Wagner MS, Tyler BJ, Castner DG. Interpretation of ToF-SIMS spectra of adsorbed protein films by multivariate pattern recognition. Anal Chem 2002:1824-1835.
42. Ryan MC, Christiano AM, Engvall E, Wewer UM, Burgeson RE. The functions of laminins: lessons from in vivo studies. Matrix Biol 1996;15:369-381.
43. Clark EA, Brugge JS. Integrins and signal-transduction pathways-the road taken. Science 1995;268:233-239.
44. Hynes RO. Integrins-versatility, modulation, and signaling in cell-adhesion. Cell 1992;69:11-25.
45. Akiyama SK, Yamada KM. Fibronectin and fibronectin fragments. In: Haralson MA, Hassel JR, editors. Extracellular matrix: a practical approach. New York: Oxford University Press; 1995. p 175-185.
46. Haralson MA, Hassel JR. The extracellular matrix-an overview. In: Haralson MA, Hassel JR, editors. Extracellular matrix: a practical approach. New York: Oxford University Press; 1995. p 1-30.
47. Hudson BG, Gunwar S, Chung AE, Burgeson RE. Matrix components (types IV and VII collagen, entactin, and laminin) found in basement membranes. In: Haralson MA, Hassel JR, editors. Extracellular matrix: a practical approach. New York: Oxford University Press; 1995. p 404.
48. Hynes R. Fibronectins. In: Kreis T, Vale R, editors. Guidebook to the extracellular matrix, anchor, and adhesion proteins. Oxford, UK: Sambrook & Tooze at Oxford University Press; 1999. p 422-425.
49. Olsen BR, Ninomiya Y. Basement membrane collagens. In: Kreis T, Vale R, editors. Guidebook to the extracellular matrix, anchor, and adhesion proteins. Oxford, UK: Sambrook & &Tooze at Oxford University Press; 1999. p 395-398.
50. Oh SP, Kamagata Y, Muragaki Y, Timmons S, Ooshima A, Olsen BR. Proc Natl Acad Sci USA 1994;91(10):4229-4233.
51. Aumailley M, Timpl R. Attachment of cells to basement-membrane collagen type-IV. J Cell Biol 1986;103:1569-1575.
52. Canfield AE, Wren FE, Schor SL, Grant ME, Schor AM. Aortic endothelial cell heterogeneity in vitro. J Cell Sci 1992;102:804-814.
53. Canfield AE, Schor AM. Heterogeneity in collagen biosynthesis by sprouting retinal endothelial cells. J Cell Physiol 1994; 159:19-28.
54. Kushida A, Yamato M, Konno C, Kikuchi A, Sakurai Y, Okano T. Decrease in culture temperature releases monolayer endothelial cell sheets together with deposited fibronectin matrix from temperature-responsive culture surfaces. J Biomed Mater Res 1999;45:355-362.
55. Cheng X, Canavan HE, Wenzel MJ, Hull J, Kweskin SJ, Talib RA, Wagner MS, McArthur SL, Somorjai GA, Castner DG, et al. Characterization of the surface chemical and mechanical properties of plasma polymerized N-isopropyl acrylamide. Langmuir. Forthcoming.
56. Michel R, Luginbuhl R, Graham DJ, Ratner BD. Self-assembled monolayers for polymer and protein cationization with time-of-flight secondary ion mass spectrometry. J Vacuum Sci Technol A 2000;18:1114-1118.
57. Pettit DK, Horbett TA, Hoffman AS. Influence of the substrate binding characteristics of fibronectin on corneal epithelial-cell outgrowth. J Biomed Mater Res 1992;26:1259-1275.
58. Voet D, Voet JG. Biochemistry. New York: John Wiley & Sons; 1990. 1223 p.
59. Horbett TA. Principles underlying the role of adsorbed plasma-proteins in blood interactions with foreign materials. Cardiovasc Pathol 1993;2:S137-148.
60. Cheng X, Canavan HE, Castner DG, Ratner BD. Protein adsorption on plasma polymerized NIPAM coating. Manuscript in preparation.
61. Yamato M, Konno C, Kushida A, Hirose M, Utsumi M, Kikuchi A, Okano T. Release of adsorbed fibronectin from temperature-responsive culture surfaces requires cellular activity. Biomaterials 2000;21:981-986.