Validation and comparison of different end point and real time RT-PCR assays for detection and genotyping of porcine reproductive and respiratory syndrome virus

Journal of Virological Methods

Volumn 201, Issue , 2014, Pages 79-85

  Drigo, Michele ^a   Franzo, Giovanni ^a   Belfanti, Ilaria ^b   Martini, Marco ^a   Mondin, Alessandra ^a   Ceglie, Letizia ^b  

^a UNIVERSITY OF PADOVA   (Italy)

^b ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELLE VENEZIE   (Italy)

Author keywords

Genotyping; PRRSV; QRT PCR; RT PCR

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTERIVIRUS; ARTICLE; ASSAY; CELL CULTURE; COMPARATIVE STUDY; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; GENOTYPE; INTERNAL CONTROL SYSTEM; MEASUREMENT REPEATABILITY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SERUM; SYBR GREEN ONE STEP REAL TIME POLYMERASE CHAIN REACTION; TAQMAN BASED ONE STEP REAL TIME POLYMERASE CHAIN REACTION; VALIDATION PROCESS; VIREMIA; VIRUS DETECTION; ANIMAL; CLASSIFICATION; EVALUATION STUDY; GENETICS; GENOTYPING TECHNIQUE; ISOLATION AND PURIFICATION; MOLECULAR DIAGNOSIS; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PROCEDURES; REPRODUCIBILITY; SWINE;

ANIMALS; GENOTYPING TECHNIQUES; MOLECULAR DIAGNOSTIC TECHNIQUES; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SWINE;

EID: 84896517107     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2014.02.015     Document Type: Article

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