Surface engineering of a cutinase from Thermobifida Cellulosilytica for improved polyester hydrolysis

Biotechnology and Bioengineering

Volumn 110, Issue 10, 2013, Pages 2581-2590

  Herrero Acero, Enrique ^a   Ribitsch, Doris ^a   Dellacher, Anita ^a   Zitzenbacher, Sabine ^a   Marold, Annemarie ^a   Steinkellner, Georg ^a   Gruber, Karl ^a,c   Schwab, Helmut ^a,b   Guebitz, Georg M ^a,d  

^a AUSTRIAN CENTRE OF INDUSTRIAL BIOTECHNOLOGY   (Austria)

^b GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^c UNIVERSITY OF GRAZ   (Austria)

^d UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

PET degradation; Polyesterase; Surface engineering

Indexed keywords

ESCHERICHIA COLI; HYDROLYSIS; SUBSTRATES; SURFACE CHEMISTRY; SURFACE PROPERTIES; SURFACES;

HYDROLYSIS ACTIVITY; HYDROPHOBIC SURFACES; NEGATIVELY CHARGED; POLYESTERASE; POSITIVELY CHARGED; SITE DIRECTED MUTAGENESIS; SOLUBLE SUBSTRATES; SURFACE ENGINEERING;

AMINO ACIDS;

ARGININE; ASPARAGINE; CUTINASE; GLUTAMIC ACID; GLUTAMINE; POLYETHYLENE TEREPHTHALATE; BACTERIAL PROTEIN; CARBOXYLESTERASE; POLYESTER;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIUM MUTANT; BIOENGINEERING; CONTROLLED STUDY; ENZYME KINETICS; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; HYDROLYSIS; MOLECULAR MODEL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; SURFACE PROPERTY; THERMOBIFIDA; THERMOBIFIDA CELLULOSILYTICA; WILD TYPE; ACTINOMYCETALES; BIOTECHNOLOGY; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; METHODOLOGY; MUTATION; PET DEGRADATION; POLYESTERASE; PROTEIN TERTIARY STRUCTURE; SURFACE ENGINEERING; PROCEDURES;

THERMOBIFIDA; THERMOBIFIDA CELLULOSILYTICA;

PET DEGRADATION; POLYESTERASE; SURFACE ENGINEERING;

ACTINOMYCETALES; BACTERIAL PROTEINS; BIOTECHNOLOGY; CARBOXYLIC ESTER HYDROLASES; HYDROLYSIS; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; POLYESTERS; PROTEIN STRUCTURE, TERTIARY; SURFACE PROPERTIES;

EID: 84896419055     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24930     Document Type: Article

References (35)

1. Anonymous. 1999. Chapter 1. Kinds of fibres. In: Karmakar SR, editor. Textile science and technology chemical techonology in the pre-treatment processes of textiles. Amsterdam, The Netherlands: Elsevier. p 1-48.
2. Akovali G. 2005. Handbook of polymers in construction. Shrewsbury: Rapra Technology Limited.
3. Alisch-Mark M, Herrmann A, Zimmermann W. 2006. Increase of the hydrophilicity of polyethylene terephthalate fibers by hydrolases from Thermomonospora fusca and Fusarium solani f. sp pisi. Biotechnol Lett 28:681-685.
4. Araujo R, Silva C, O'Neill A, Micaelo N, Guebitz G, Soares CM, Casal M, Cavaco-Paulo A. 2007. Tailoring cutinase activity towards polyethylene terephthalate and polyamide 6, 6 fibers. J Biotechnol 128:849-857.
5. Brueckner T, Eberl A, Heumann S, Rabe M, Guebitz GM. 2008. Enzymatic and chemical hydrolysis of poly(ethylene terephthalate) fabrics. J Polym Sci [A1] 46:6435-6443.
6. Canutescu AA, Shelenkov AA, Dunbrack RL, Jr. 2003. A graph-theory algorithm for rapid protein side-chain prediction. Protein Sci 12:2001-2014.
7. Correa A, Oppezzo P. 2011. Tuning different expression parameters to achieve soluble recombinant proteins in E.coli: Advantage of high-throughput screening. Biotechnol J 6:715-730.
8. Donelli I, Freddi G, Nierstrasz VA, Taddei P. 2010. Surface structure and properties of poly-(ethylene terephthalate) hydrolyzed by alkali and cutinase. Polym Degrad Stab 95:1542-1550.
9. Duan Y, Wu C, Chowdhury S, Lee MC, Xiong G, Zhang W, Yang R, Cieplak P, Luo R, Lee T, Caldwell J, Wang J, Kollman P. 2003. A point-charge force field for molecular mechanics simulations of proteins based on condensed-phase quantum mechanical calculations. J Comput Chem 24:1999-2012.
10. Dunbrack RL, Jr., Cohen FE. 1997. Bayesian statistical analysis of protein side-chain rotamer preferences. Protein Sci 6:1661-1681.
11. Eberl A, Heumann S, Brueckner T, Araújo R, Cavaco-Paulo A, Kaufmann F. 2009. Enzymatic surface hydrolysis of poly(ethylene terephthalate) and bis(benzoyloxyethyl) terephthalate by lipase and cutinase in the presence of surface active molecules. J. Biotechnol 143:207-212.
12. Guebitz GM, Cavaco-Paulo A. 2008. Enzymes go big: Surface hydrolysis and functionalisation of synthetic polymers. Trends Biotechnol 26:32-38.
13. Gusakov AV, Sinitsyn AP, Berlin AG, Markov AV, Ankudimova NV. 2000. Surface hydrophobic amino acid residues in cellulase molecules as a structural factor responsible for their high denim-washing performance. Enzyme Microb Technol 27:664-671.
14. Herrero Acero E, Ribitsch D, Steinkellner G, Gruber K, Greimel K, Eiteljoerg I, Trotscha E, Wei R, Zimmermann W, Zinn M, Cavaco-Paulo A, Freddi G, Schwab H, Guebitz G. 2011. Enzymatic surface hydrolysis of PET: Effect of structural diversity on kinetic properties of cutinases from Thermobifida cellulosilytica. Macromol 44:4640.
15. Heumann S, Eberl A, Pobeheim H, Liebminger S, Fischer-Colbrie G, Almansa E, Cavaco-Paulo A, Guebitz GM. 2006. New model substrates for enzymes hydrolysing polyethyleneterephthalate and polyamide fibers. J Biochem Bioph Methods 69:89-8999.
16. Honig B, Nicholls A. 1995. Classical electrostatics in biology and chemistry. Science 268:1144-1149.
17. Huang CJ, Lin H, Yang X. 2012. Industrial production of recombinant therapeutics on Escherichia coli and its recent advancements. J Ind Microbiol Biotechnol 39:383-399.
18. Kellis J, Estell D, Cascão-Pereira L. 2012. Conference Book, EMBO conference catalytic mechanism by biological systems, 7-10 October 2012, page 45.
19. Kitadokoro K, Thumarat U, Nakamura R, Nishimura K, Karatani H, Suzuki H, Kawai F. 2012. Crystal structure of cutinase Est119 from Thermobifida alba AHK119 that can degrade modified polyethylene terephthalate at 1.76 Å resolution. Polym Degradation Stab 97:771-775.
20. Krieger E, Darden T, Nabuurs SB, Finkelstein A, Vriend G. 2004. Making optimal use of empirical energy functions: Force-field parameterization in crystal space. Proteins 57:678-683.
21. Krieger E, Koraimann G, Vriend G. 2002. Increasing the precision of comparative models with YASARA NOVA - a self-parameterizing force field. Proteins 47:393-402.
22. Nimchua T, Punnapayak H, Zimmermann W. 2007. Comparison of the hydrolysis of polyethylene terephthalate fibers by a hydrolase from Fusarium oxysporum LCH I and Fusarium solani f. sp. pisi. Biotechnol J 2:361-364.
23. Oeser T, Wei R, Baumgarten T, Billig S, Foellner C, Zimmermann W. 2010. High level expression of a hydrophobic poly(ethylene terephthalate) hydrolyzing carboxylesterase from Thermobifida fusca KW3 in Escherichia coli BL21(DE3). J Biotechnol 146:100-104.
24. Ribitsch D, Herrero Acero E, Greimel K, Dellacher A, Zitzenbacher S, Marold A, Diaz Rodriguez R, Schwab H, Guebitz GM. 2012a. A new esterase from Thermobifida halotolerans hydrolyses PET and PLA. Polymers 4(1):617-629.
25. Ribitsch D, Herrero Acero E, Greimel K, Eiteljoerg I, Trotscha E, Freddi G, Schwab H, Guebitz GM. 2012b. Characterization of a new cutinase from Thermobifida alba regarding PET-surface hydrolysis. Biocatal Biotransform 30:2-9.
26. Ribitsch D, Heumann S, Trotscha E, Herrero Acero E, Greimel K, Leber R, Birner-Gruenberger R, Deller S, Eiteljoerg I, Remler P, Weber T, Siegert P, Maurer K, Donelli I, Freddi G, Schwab H, Guebitz GM. 2011. Hydrolysis of polyethyleneterephthalate by para-nitrobenzylesterase from Bacillus subtilis. Biotechnol Prog 27:951-960.
27. Ricco J, Assadin A, Schneider F, Assadian O. 2012. In vitro evaluation of the antimicrobial efficacy of a new silver-triclosan vs a silver collagen-coated polyester vascular graft against methicillin-resistant Staphylococcus aureus. J Vasc Surg 55:823-829.
28. Sambrook JE, Fritsch EF, Maniatis T. 1989. Molecular cloning: A laboratory manual. Cold Spring Harbor, New York: Cold Spring Harbor Press.
29. Sanner MF, Olson AJ, Spehner JC. 1996. Reduced surface: An efficient way to compute molecular surfaces. Biopolymers 38:305-320.
30. Schrödinger L. 2011. The PyMOL Molecular Graphics System, Version~1.41.
31. Schroeder M, Aichernig N, Guebitz GM, Kokol V. 2007. Enzymatic coating of lignocellulosic surfaces polyphenols. Biotechnol J 2:334-341.
32. Silva C, Da S, Silva N, Matamá T, Araújo R, Martins M, Chen S, Chen J, Wu J, Casal M, Cavaco-Paulo A. 2011. Engineered Thermobifida fusca cutinase with increased activity on polyester substrates. Biotechnol J 6:1230-1239.
33. Steinkellner G, Rader R, Thallinger GG, Kratky C, Gruber K. 2009. VASCo: Computation and visualization of annotated protein surface contacts. BMC Bioinformatics 10:1-11.
34. Wei Y, Swenson L, Castro C, Derewenda U, Minor W, Arai H, Aoki J, Inoue K, Servin-Gonzalez L, Derewenda ZS. 1998. Structure of a microbial homologue of mammalian platelet-activating factor acetylhydrolases: Streptomyces exfoliatus lipase at 1.9 Å resolution. Structure 6:511-519.
35. Zeronian SH, Collins MJ. 1989. Surface modification of polyester by alkaline treatments. Textile Prog 20:1-34.