Reconstitution of microtubule-dependent organelle transport

Methods in Enzymology

Volumn 540, Issue , 2014, Pages 231-248

  Barak, Pradeep ^a   Rai, Ashim ^a   Dubey, Alok Kumar ^a   Rai, Priyanka ^a   Mallik, Roop ^a  

^a TATA INSTITUTE OF FUNDAMENTAL RESEARCH   (India)

Author keywords

In vitro motility; Lipid droplets; Optical trap; Organelle motility; Phagosomes

Indexed keywords

CELL EXTRACT; FAT DROPLET; FATTY ACID;

ARTICLE; CELL ISOLATION; CELL MATURATION; CELL MOTILITY; CELL TRANSPORT; DICTYOSTELIUM; ENDOSOME; FATTY ACID METABOLISM; IN VITRO STUDY; LATEX BEAD PHAGOSOME; LIVER; MICROTUBULE; NONHUMAN; PHAGOSOME; PRIORITY JOURNAL; PURIFICATION;

IN VITRO MOTILITY; LIPID DROPLETS; OPTICAL TRAP; ORGANELLE MOTILITY; PHAGOSOMES;

ANIMALS; BIOLOGICAL TRANSPORT; DICTYOSTELIUM; LIPID METABOLISM; LIPIDS; LIVER; MICROTUBULES; OPTICAL TWEEZERS; ORGANELLES; PHAGOSOMES; RATS;

EID: 84896331175     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-397924-7.00013-3     Document Type: Chapter

References (41)

1. P. Barak, A. Rai, P. Rai, and R. Mallik Quantitative optical trapping on single organelles in cell extract Nature Methods 10 1 2013 68 70
2. K. Barlan, M.J. Rossow, and V.I. Gelfand The journey of the organelle: Teamwork and regulation in intracellular transport Current Opinion in Cell Biology 25 4 2013 483 488
3. T.F. Bartsch, R.A. Longoria, E.L. Florin, and G.T. Shubeita Lipid droplets purified from Drosophila embryos as an endogenous handle for precise motor transport measurements Biophysical Journal 105 5 2013 1182 1191
4. A. Blocker, F.F. Severin, J.K. Burkhardt, J.B. Bingham, H. Yu, and J.C. Olivo et al. Molecular requirements for bi-directional movement of phagosomes along microtubules The Journal of Cell Biology 137 1 1997 113 129
5. S.T. Brady, R.J. Lasek, and R.D. Allen Fast axonal transport in extruded axoplasm from squid giant axon Science 218 4577 1982 1129 1131
6. D.L. Brasaemle, G. Dolios, L. Shapiro, and R. Wang Proteomic analysis of proteins associated with lipid droplets of basal and lipolytically stimulated 3 T3-L1 adipocytes The Journal of Biological Chemistry 279 45 2004 46835 46842
7. D.L. Brasaemle, and N.E. Wolins Isolation of lipid droplets from cells by density gradient centrifugation Current Protocols in Cell Biology 2006 Chapter 3: Unit 3 15
8. M. Castoldi, and A.V. Popov Purification of brain tubulin through two cycles of polymerization-depolymerization in a high-molarity buffer Protein Expression and Purification 32 1 2003 83 88
9. M. Desjardins, and G. Griffiths Phagocytosis: Latex leads the way Current Opinion in Cell Biology 15 4 2003 498 503
10. R.V. Farese Jr., and T.C. Walther Lipid droplets finally get a little R-E-S-P-E-C-T Cell 139 5 2009 855 860
11. J.M. Goodman The gregarious lipid droplet The Journal of Biological Chemistry 283 42 2008 28005 28009
12. S.P. Gross, M. Vershinin, and G.T. Shubeita Cargo transport: Two motors are sometimes better than one Current Biology 17 12 2007 R478 R486
13. E. Herker, and M. Ott Emerging role of lipid droplets in host/pathogen interactions The Journal of Biological Chemistry 287 4 2012 2280 2287
14. T. Kon, T. Oyama, R. Shimo-Kon, K. Imamula, T. Shima, and K. Sutoh et al. The 2.8 a crystal structure of the dynein motor domain Nature 484 7394 2012 345 350
15. M.P. Koonce Dictyostelium, a model organism for microtubule-based transport Protist 151 1 2000 17 25
16. G.E. Lee, J.W. Murray, A.W. Wolkoff, and D.W. Wilson Reconstitution of herpes simplex virus microtubule-dependent trafficking in vitro Journal of Virology 80 9 2006 4264 4275
17. R. Mallik, B.C. Carter, S.A. Lex, S.J. King, and S.P. Gross Cytoplasmic dynein functions as a gear in response to load Nature 427 2004 649 652
18. R. Mallik, and S.P. Gross Molecular motors: Strategies to get along Current Biology 14 2004 R971 R982
19. R. Mallik, A.K. Rai, P. Barak, A. Rai, and A. Kunwar Teamwork in microtubule motors Trends in Cell Biology 23 2013 575 582
20. J.W. Murray, E. Bananis, and A.W. Wolkoff Reconstitution of ATP-dependent movement of endocytic vesicles along microtubules in vitro: An oscillatory bidirectional process Molecular Biology of the Cell 11 2 2000 419 433
21. N. Pollock, E.L. de Hostos, C.W. Turck, and R.D. Vale Reconstitution of membrane transport powered by a novel dimeric kinesin motor of the Unc104/KIF1A family purified from Dictyostelium The Journal of Cell Biology 147 1999 493 506
22. N. Pollock, M.P. Koonce, E.L. de Hostos, and R.D. Vale In vitro microtubule-based organelle transport in wild-type Dictyostelium and cells overexpressing a truncated dynein heavy chain Cell Motility and the Cytoskeleton 40 1998 304 314
23. A.K. Rai, A. Rai, A.J. Ramaiya, R. Jha, and R. Mallik Molecular adaptations allow dynein to generate large collective forces inside cells Cell 152 1-2 2013 172 182
24. S.E. Rice, T.J. Purcell, and J.A. Spudich Building and using optical traps to study properties of molecular motors Methods in Enzymology 361 2003 112 133
25. S.L. Rogers, I.S. Tint, P.C. Fanapour, and V.I. Gelfand Regulated bidirectional motility of melanophore pigment granules along microtubules in vitro Proceedings of the National Academy of Sciences of the United States of America 94 8 1997 3720 3725
26. S. Romagnoli, G. Cai, and M. Cresti In vitro assays demonstrate that pollen tube organelles use kinesin-related motor proteins to move along microtubules Plant Cell 15 1 2003 251 269
27. A. Rupper, B. Grove, and J. Cardelli Rab7 regulates phagosome maturation in Dictyostelium Journal of Cell Science 114 Pt 13 2001 2449 2460
28. G.T. Shubeita, S.L. Tran, J. Xu, M. Vershinin, S. Cermelli, and S.L. Cotton et al. Consequences of motor copy number on the intracellular transport of kinesin-1-driven lipid droplets Cell 135 6 2008 1098 1107
29. T.E. Sladewski, C.S. Bookwalter, M.S. Hong, and K.M. Trybus Single-molecule reconstitution of mRNA transport by a class V myosin Nature Structural & Molecular Biology 20 8 2013 952 957
30. V. Soppina, A. Rai, and R. Mallik Simple non-fluorescent polarity labeling of Microtubules for Molecular Motor assays Biotechniques 46 2009 297 303
31. V. Soppina, A.K. Rai, A.J. Ramaiya, P. Barak, and R. Mallik Tug-of-war between dissimilar teams of microtubule motors regulates transport and fission of endosomes Proceedings of the National Academy of Sciences of the United States of America 106 46 2009 19381 19386
32. K. Svoboda, C.F. Schmidt, B.J. Schnapp, and S.M. Block Direct observation of kinesin stepping by optical trapping interferometry Nature 365 6448 1993 721 727
33. P. Targett-Adams, D. Chambers, S. Gledhill, R.G. Hope, J.F. Coy, and A. Girod et al. Live cell analysis and targeting of the lipid droplet-binding adipocyte differentiation-related protein The Journal of Biological Chemistry 278 18 2003 15998 16007
34. A.R. Thiam, B. Antonny, J. Wang, J. Delacotte, F. Wilfling, and T.C. Walther et al. COPI buds 60-nm lipid droplets from reconstituted water-phospholipid-triacylglyceride interfaces, suggesting a tension clamp function Proceedings of the National Academy of Sciences of the United States of America 110 33 2013 13244 13249
35. R.D. Vale The molecular motor toolbox for intracellular transport Cell 112 2003 467 480
36. R.D. Vale, B.J. Schnapp, T.S. Reese, and M.P. Sheetz Movement of organelles along filaments dissociated from the axoplasm of the squid giant axon Cell 40 2 1985 449 454
37. R.B. Vallee, R.J. McKenney, and K.M. Ori-McKenney Multiple modes of cytoplasmic dynein regulation Nature Cell Biology 14 3 2012 224 230
38. K.J. Verhey, and J.W. Hammond Traffic control: Regulation of kinesin motors Nature Reviews. Molecular Cell Biology 10 11 2009 765 777
39. H. Wang, E. Wei, A.D. Quiroga, X. Sun, N. Touret, and R. Lehner Altered lipid droplet dynamics in hepatocytes lacking triacylglycerol hydrolase expression Molecular Biology of the Cell 21 12 2010 1991 2000
40. M.A. Welte Bidirectional transport along microtubules Current Biology 14 2004 R525 R537
41. M.A. Welte Fat on the move: Intracellular motion of lipid droplets Biochemical Society Transactions 37 Pt 5 2009 991 996