Noninvasive imaging of Staphylococcus aureus infections with a nuclease-activated probe

Nature Medicine

Volumn 20, Issue 3, 2014, Pages 301-306

  Hernandez, Frank J ^a   Huang, Lingyan ^b   Olson, Michael E ^a   Powers, Kristy M ^b   Hernandez, Luiza I ^a   Meyerholz, David K ^a   Thedens, Daniel R ^a   Behlke, Mark A ^b   Horswill, Alexander R ^a   Mcnamara, James O ^a  

^a UNIVERSITY OF IOWA   (United States)

^b INTEGRATED DNA TECHNOLOGIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MICROCOCCAL NUCLEASE; NUCLEASE; OLIGONUCLEOTIDE; THYMIDINE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIUM CULTURE; CHEMICAL MODIFICATION; CONTROLLED STUDY; FLUORESCENCE; MAMMAL; MOLECULAR IMAGING; MOUSE; MYOSITIS; NON INVASIVE PROCEDURE; NONHUMAN; NUCLEIC ACID PROBE; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS INFECTION; SUPERNATANT;

ANIMALIA; BACTERIA (MICROORGANISMS); MAMMALIA; MUS; STAPHYLOCOCCUS AUREUS;

ANIMALS; BACTERIOPHAGES; FEMALE; FLUORESCENCE; FLUORESCENT DYES; KINETICS; MAGNETIC RESONANCE IMAGING; MICE; MICE, INBRED C57BL; MICROCOCCAL NUCLEASE; OLIGONUCLEOTIDE PROBES; OLIGONUCLEOTIDES; PYOMYOSITIS; STAPHYLOCOCCAL INFECTIONS;

EID: 84896135619     PISSN: 10788956     EISSN: 1546170X     Source Type: Journal    
DOI: 10.1038/nm.3460     Document Type: Article

References (41)

1. Agarwal, V., Chauhan, S. & Gupta, R.K. Pyomyositis. Neuroimaging Clin. N. Am. 21, 975-983 (2011
2. Carek, P.J., Dickerson, L.M. & Sack, J.L. Diagnosis and management of osteomyelitis. Am. Fam. Physician 63, 2413-2420 (2001
3. Clerc, O., Prod'hom, G., Greub, G., Zanetti, G. & Senn, L. Adult native septic arthritis: A review of 10 years of experience and lessons for empirical antibiotic therapy. J. Antimicrob. Chemother. 66, 1168-1173 (2011
4. Hatzenbuehler, J. & Pulling, T.J. Diagnosis and management of osteomyelitis. Am. Fam. Physician 84, 1027-1033 (2011
5. Hope-Roberts, M., Wainwright, M. & Horobin, R.W. Real-Time imaging of bacteria in living mice using a fluorescent dye. Biotech. Histochem. 86, 104-107 (2011
6. Leevy, W.M. et al. Optical imaging of bacterial infection in living mice using a fluorescent near-infrared molecular probe. J. Am. Chem. Soc. 128, 16476-16477 (2006
7. Leevy, W.M. et al. Noninvasive optical imaging of Staphylococcus aureus bacterial infection in living mice using a bis-dipicolylamine-Zinc(ii) affinity group conjugated to a near-infrared fluorophore. Bioconjug. Chem. 19, 686-692 (2008
8. Ning, X. et al. Maltodextrin-based imaging probes detect bacteria in vivo with high sensitivity and specificity. Nat. Mater. 10, 602-607 (2011
9. Panizzi, P. et al. In vivo detection of Staphylococcus aureus endocarditis by targeting pathogen-specific prothrombin activation. Nat. Med. 17, 1142-1146 (2011
10. White, A.G. et al. Optical imaging of bacterial infection in living mice using deep-red fluorescent squaraine rotaxane probes. Bioconjug. Chem. 21, 1297-1304 (2010
11. Kong, Y. et al. Imaging tuberculosis with endogenous β-lactamase reporter enzyme fluorescence in live mice. Proc. Natl. Acad. Sci. USA 107, 12239-12244 (2010
12. Yang, W. Nucleases: Diversity of structure, function and mechanism. Q. Rev. Biophys. 44, 1-93 (2011
13. Kelemen, B.R. et al. Hypersensitive substrate for ribonucleases. Nucleic Acids Res. 27, 3696-3701 (1999
14. Elias, D.R., Thorek, D.L., Chen, A.K., Czupryna, J. & Tsourkas, A. In vivo imaging of cancer biomarkers using activatable molecular probes. Cancer Biomark. 4, 287-305 (2008
15. Hernandez, F.J. et al. Degradation of nuclease-stabilized RNA oligonucleotides in Mycoplasma-contaminated cell culture media. Nucleic Acid Ther. 22, 58-68 (2012
16. Arnone, A. et al. The extracellular nuclease of Staphylococcus aureus: Structures of the native enzyme and an enzyme-inhibitor complex at 4 A resolution. Proc. Natl. Acad. Sci. USA 64, 420-427 (1969
17. Cuatrecasas, P., Fuchs, S. & Anfinsen, C.B. Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus. J. Biol. Chem. 242, 1541-1547 (1967
18. Cunningham, L., Catlin, B.W. & Privatdegarilhe, M. A deoxyribonuclease of Micrococcus pyogenes. J. Am. Chem. Soc. 78, 4642-4645 (1956
19. Boerlin, P., Kuhnert, P., Hussy, D. & Schaellibaum, M. Methods for identification of Staphylococcus aureus isolates in cases of bovine mastitis. J. Clin. Microbiol. 41, 767-771 (2003
20. Behlke, M.A. Chemical modification of siRNAs for in vivo use. Oligonucleotides 18, 305-319 (2008
21. Vester, B. & Wengel, J. LNA (locked nucleic acid): High-Affinity targeting of complementary RNA and DNA. Biochemistry 43, 13233-13241 (2004
22. Pochon, F. & Privat De Garilhe, M. Bull. Soc. Chim. Biol. (Paris) [Structure of oligonucleotides liberated by the action of desoxyribonuclease on Staphylococcus pyogenes] 42, 795-815 (1960
23. Kiedrowski, M.R. et al. Nuclease modulates biofilm formation in community-Associated methicillin-resistant Staphylococcus aureus. PLoS ONE 6, e26714 (2011
24. Beenken, K.E. et al. Epistatic relationships between sarA and agr in Staphylococcus aureus biofilm formation. PLoS ONE 5, e10790 (2010
25. Weissleder, R. & Ntziachristos, V. Shedding light onto live molecular targets. Nat. Med. 9, 123-128 (2003
26. Contag, C.H. et al. Photonic detection of bacterial pathogens in living hosts. Mol. Microbiol. 18, 593-603 (1995
27. Francis, K.P. et al. Monitoring bioluminescent Staphylococcus aureus infections in living mice using a novel luxABCDE construct. Infect. Immun. 68, 3594-3600 (2000
28. Xiong, Y.Q. et al. Real-Time in vivo bioluminescent imaging for evaluating the efficacy of antibiotics in a rat Staphylococcus aureus endocarditis model. Antimicrob. Agents Chemother. 49, 380-387 (2005
29. Beenken, K.E., Spencer, H., Griffin, L.M. & Smeltzer, M.S. Impact of extracellular nuclease production on the biofilm phenotype of Staphylococcus aureus under in vitro and in vivo conditions. Infect. Immun. 80, 1634-1638 (2012
30. De Marie, S. Difficult-To-Treat infections. Intensive Care Med. 16 (suppl. 3), S239-S242 (1990
31. Wu, P.I. & Edelman, E.R. Structural biomechanics modulate intramuscular distribution of locally delivered drugs. J. Biomech. 41, 2884-2891 (2008
32. Ferreira, B.T., Benchetrit, L.C., De Castro, A.C., Batista, T.G. & Barrucand, L. Extracellular deoxyribonucleases of streptococci: A comparison of their occurrence and levels of production among β-hemolytic strains of various serological groups. Zentralbl. Bakteriol. 277, 493-503 (1992
33. Ferrieri, P., Gray, E.D. & Wannamaker, L.W. Biochemical and immunological characterization of the extracellular nucleases of group B streptococci. J. Exp. Med. 151, 56-68 (1980
34. Moon, A.F. et al. Structural insights into catalytic and substrate binding mechanisms of the strategic EndA nuclease from Streptococcus pneumoniae. Nucleic Acids Res. 39, 2943-2953 (2011
35. Marshall, M.V., Draney, D., Sevick-Muraca, E.M. & Olive, D.M. Single-dose intravenous toxicity study of IRDye 800CW in Sprague-Dawley rats. Mol. Imaging Biol. 12, 583-594 (2010
36. Kobayashi, H., Longmire, M.R., Ogawa, M. & Choyke, P.L. Rational chemical design of the next generation of molecular imaging probes based on physics and biology: Mixing modalities, colors and signals. Chem. Soc. Rev. 40, 4626-4648 (2011
37. Matsui, A. et al. Real-Time, near-infrared, fluorescence-guided identification of the ureters using methylene blue. Surgery 148, 78-86 (2010
38. Rasmussen, J.C. et al. Human lymphatic architecture and dynamic transport imaged using near-infrared fluorescence. Transl. Oncol. 3, 362-372 (2010
39. Sevick-Muraca, E.M. Translation of near-infrared fluorescence imaging technologies: Emerging clinical applications. Annu. Rev. Med. 63, 217-231 (2012
40. Novick, R.P. Genetic systems in staphylococci. Methods Enzymol. 204, 587-636 (1991
41. Stoltz, D.A. et al. Cystic fibrosis pigs develop lung disease and exhibit defective bacterial eradication at birth. Sci. Transl. Med. 2, 29ra31 (2010