Analysis of Rheb in the cellular slime mold Dictyostelium discoideum: Cellular localization, spatial expression and overexpression

Journal of Biosciences

Volumn 39, Issue 1, 2014, Pages 75-84

  Swer, Pynskhem Bok ^a   Bhadoriya, Pooja ^a   Saran, Shweta ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

Dictyostelium; Rheb; TOR signalling

Indexed keywords

BETA GALACTOSIDASE; COMPLEMENTARY DNA; GENOMIC DNA; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE KINASE; INITIATION FACTOR 4E BINDING PROTEIN 1; RAPAMYCIN; RHEB PROTEIN; TARGET OF RAPAMYCIN KINASE;

CYTOPLASM; FLUORESCENCE; HYBRIDIZATION; POLYMERASE CHAIN REACTION; PROTEIN; SLIME MOULD; SPATIAL ANALYSIS;

AMINO ACID SUBSTITUTION; ARTICLE; AUTOPHAGY; CELL SIZE; CELL TYPE; CELLULAR DISTRIBUTION; COMPUTER MODEL; CONTROLLED STUDY; DICTYOSTELIUM DISCOIDEUM; ENZYME ACTIVITY; FRUITING BODY; FUNGAL CELL; FUNGAL DEVELOPMENT; GENE OVEREXPRESSION; HUMAN; IN SITU HYBRIDIZATION; INTRACELLULAR SIGNALING; MOLECULAR DYNAMICS; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENETIC TREE; PRESTALK CELL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHEB GENE; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

ACRASIDA; DICTYOSTELIUM; DICTYOSTELIUM DISCOIDEUM;

BACTERIAL PROTEINS; BASE SEQUENCE; CLUSTER ANALYSIS; DICTYOSTELIUM; DNA PRIMERS; GENE EXPRESSION PROFILING; IMMUNOBLOTTING; IN SITU HYBRIDIZATION; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; MONOMERIC GTP-BINDING PROTEINS; NEUROPEPTIDES; PHYLOGENY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SIGNAL TRANSDUCTION; TOR SERINE-THREONINE KINASES;

EID: 84896050902     PISSN: 02505991     EISSN: 09737138     Source Type: Journal    
DOI: 10.1007/s12038-013-9405-9     Document Type: Article

References (29)

1. Aspuria PJ and Tamanoi F 2004 The Rheb family of GTP-binding proteins. Cell. Signal. 16 1105-11012
2. Buerger C, DeVries B and Stambolic V 2006 Localization of Rheb to the endomembrane is critical for its signaling function. Biochem. Biophys. Res. Commun. 344 869-880
3. Clark GJ, Kinch MS, Rogers-Graham K, Sebti SM, Hamilton AD and Der CJ 1997 The Ras-related protein Rheb is farnesylated and antagonizes Ras signaling and transformation. J. Biol. Chem. 272 10608-10615 (Pubitemid 27181069)
4. Early AE, Gaskell MJ, Traynor D and Williams JG 1993 Two distinct populations of prestalk cells within the tip of the migratory Dictyostelium slug with differing fates at culmination. Development 118 353-362 (Pubitemid 23214024)
5. Gaskell M, Watts D, Treffry T, Jermyn KA and Williams JG 1992 Immunolocalization and separation of multiple prestalk cell types in Dictyostelium. Differentiation 57 171-176
6. Gosain A, Lohia R, Shrivastava A and Saran S 2012 Identification and characterization of peptide: N-glycanase from Dictyostelium discoideum. BMC Biochem. 8 13-19
7. Hay N and Sonenberg N 2004 Upstream and downstream of mTOR. Genes Dev. 18 1926-1945 (Pubitemid 39071573)
8. Im E, von Lintig FC, Chen J, Zhuang S, Qui W, Chowdhury S, Worley PF, Boss GR and Pilz RB 2002 Rheb is in a high activation state and inhibits B-Raf kinase in mammalian cells. Oncogene 21 6356-6365
9. Inoki K, Li Y, Xu T and Guan K L 2003 Rheb GTPase is a direct target of TSC2 GAP activity and regulates mTOR signaling. Genes Dev. 17 1829-1834 (Pubitemid 36944560)
10. Inoki K, Kim J and Guan KL 2012 AMPK and mTOR in Cellular Energy Homeostasis and Drug Targets. Annu. Rev. Pharmacol. Toxicol. 52 381-400
11. Jermyn KA and Williams JG 1991 An analysis of culmination in Dictyostelium using prestalk and stalk-specific cell autonomous markers. Development 111 779-787 (Pubitemid 21900235)
12. Jermyn K, Traynor D and Williams J 1996 The initiation of basal disc formation in Dictyostelium discoideum is an early event in culmination. Development 122 753-760 (Pubitemid 26095981)
13. Jiang H and Vogt PK 2008 Constitutively active Rheb induces oncogenic transformation. Oncogene 27 5729-5740
14. Kim J, Kundu M, Viollet B and Guan KL 2011 AMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1. Nat. Cell Biol. 13 132-143
15. Ma D, Bai X, Guo S and Jiang Y 2008 The switch I region of Rheb is critical for its interaction with FKBP38. J. Biol. Chem. 283 25963-25970
16. Patel PH and Tamanoi F 2006 Using Drosophila and yeast genetics to investigate a role for the Rheb GTPase in cell growth. Methods Enzymol. 407 443-454 (Pubitemid 43815968)
17. Patel PH, Thapar N, Guo L, Martinez M, Maris J, Gau CL, Lengyel JA and Tamanoi F 2003 Drosophila Rheb GTPase is required for cell cycle progression and cell growth. J. Cell Sci. 116 3601-3610 (Pubitemid 37063403)
18. Ratner D and Borth W 1983 Comparison of differentiating Dictyostelium discoideum cell types separated by an improved method of density gradient centrifugation. Exp. Cell Res. 143 1-13 (Pubitemid 13145158)
19. Rojas AM, Fuentes G, Rausell A and Valencia A 2012 The Ras protein superfamily: Evolutionary tree and role of conserved amino acids. J. Cell Biol. 196 189-201
20. Rosel D, Khurana T, Majithia A, Huang X, Bhandari R and Kimmel AR 2012 TOR complex 2 (TORC2) in Dictyostelium suppresses phagocytic nutrient capture independently of TORC1mediated nutrient sensing. J. Cell Sci. 125 37-48
21. Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled L and Sabatini DM 2008 The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. Science 320 1496-1501 (Pubitemid 351929429)
22. Saucedo LJ, Gao X, Chiarelli DA, Li L, Pan D and Edgar BA 2003 Rheb promotes cell growth as a component of the insulin/TOR signaling network. Nat. Cell Biol. 5 566-571 (Pubitemid 36781092)
23. Stocker H, Radimerski T, Schindelholz B, Wittwer F, Belawat P, Daram P, Breuer S, Thomas G and Hafen E 2003 Rheb is an essential regulator of S6K in controlling cell growth in Drosophila. Nat. Cell Biol. l5 559-565 (Pubitemid 36781091)
24. Tamanoi F 2011 Ras signaling in yeast. Genes Cancer 2 210-215
25. Urano J, Ellis C, Clark GJ and Tamanoi F 2001 Characterization of Rheb functions using yeast and mammalian systems. Methods Enzymol. 333 217-231
26. van Dam TJ, Bos JL and Snel B 2011 Evolution of the Ras-like small GTPases and their regulators. Small GTPases 2 4-16
27. Wang X and Proud CG 2009 Nutrient control of TORC1, a cellcycle regulator. Trends Cell Biol. 19 260-267
28. Williams JG, Duffy KT, Lane DP, McRobbie SJ, Harwood AJ, Traynor D, Kay RR and Jermyn KA 1989 Origins of the prestalk-prespore pattern in Dictyostelium development. Cell 59 1157-1163 (Pubitemid 20024626)
29. Yadav RB, Burgos P, Parker AW, Iadevaia V, Proud CG, Allen RA, O'Connell JP, Jeshtadi A, Stubbs CD and Botchway SW 2013 mTOR direct interactions with Rheb-GTPase and raptor: sub-cellular localization using fluorescence lifetime imaging. BMC Cell Biol. 12 14-23