Capillary electrophoresis-based assessment of nanobody affinity and purity

Analytica Chimica Acta

Volumn 818, Issue , 2014, Pages 1-6

  Haselberg, Rob ^a,b   Oliveira, Sabrina ^a   Van der Meel, Roy ^a   Somsen, Govert W ^a,b   De Jong, Gerhardus J ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

Affinity capillary electrophoresis; Capillary coatings; Capillary electrophoresis mass spectrometry; Epidermal growth factor receptor; Llama antibody

Indexed keywords

ANTIBODIES; BIODEGRADATION; COATINGS; DISSOCIATION; ELECTROPHORETIC MOBILITY; MASS SPECTROMETRY; SODIUM COMPOUNDS;

AFFINITY CAPILLARY ELECTROPHORESIS; BACKGROUND ELECTROLYTES; CAPILLARY COATINGS; CAPILLARY ELECTROPHORESIS-MASS SPECTROMETRIES; DISSOCIATION CONSTANT; EFFECTIVE ELECTROPHORETIC MOBILITY; ELECTROPHORETIC MOBILITY SHIFT; EPIDERMAL GROWTH FACTOR RECEPTORS;

CAPILLARY ELECTROPHORESIS;

ACETIC ACID; BACKGROUND ELECTROLYTE; CAPILLARY COATING; EGA 1 ANTIBODY; ELECTROLYTE; EPIDERMAL GROWTH FACTOR RECEPTOR; EPIDERMAL GROWTH FACTOR RECEPTOR ANTIBODY; HEXADIMETHRINE BROMIDE; NANOBODY; NANOCOATING; PYROGLUTAMIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

AFFINITY CAPILLARY ELECTROPHORESIS; ANTIBODY AFFINITY; ANTIBODY STRUCTURE; ARTICLE; BINDING AFFINITY; CAPILLARY ELECTROPHORESIS; CHEMICAL COMPOSITION; CONTROLLED STUDY; ELECTROPHORETIC MOBILITY; HUMAN; HUMAN CELL; LIGAND BINDING; MASS SPECTROMETRY; NANOBODY PURITY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONTENT; PROTEIN DOMAIN; PROTEIN PROCESSING; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; SEPARATION TECHNIQUE;

LAMA (MAMMAL);

AFFINITY CAPILLARY ELECTROPHORESIS; CAPILLARY COATINGS; CAPILLARY ELECTROPHORESIS-MASS SPECTROMETRY; EPIDERMAL GROWTH FACTOR RECEPTOR; LLAMA ANTIBODY;

ACETATES; ACETIC ACID; AMINO ACID SEQUENCE; CHEMISTRY TECHNIQUES, ANALYTICAL; DEXTRAN SULFATE; ELECTROPHORESIS, CAPILLARY; HEXADIMETHRINE BROMIDE; KINETICS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PHOSPHATES; POLYVINYLS; RECEPTOR, EPIDERMAL GROWTH FACTOR; SINGLE-DOMAIN ANTIBODIES; SULFONIC ACIDS;

EID: 84895895547     PISSN: 00032670     EISSN: 18734324     Source Type: Journal    
DOI: 10.1016/j.aca.2014.01.048     Document Type: Article

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