Chemical ablation of tumor-initiating human pluripotent stem cells

Nature Protocols

Volumn 9, Issue 3, 2014, Pages 729-740

  Ben David, Uri ^a   Benvenisty, Nissim ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

CLAUDIN 6; OLEIC ACID;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CELL DEATH; CELL DIFFERENTIATION; CELL VIABILITY; CELLULAR STRESS RESPONSE; CYTOTOXICITY; ENDOPLASMIC RETICULUM STRESS; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; MOUSE; NONHUMAN; PHENOTYPE; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; TERATOMA; TUMOR ABLATION;

ABLATION TECHNIQUES; CELL SEPARATION; CLAUDINS; ENTEROTOXINS; HUMANS; NEOPLASTIC STEM CELLS; PLURIPOTENT STEM CELLS; REGENERATIVE MEDICINE; STEAROYL-COA DESATURASE;

EID: 84895484925     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.050     Document Type: Article

References (37)

1. Ben-David, U., Kopper, O. & Benvenisty, N. Expanding the boundaries of embryonic stem cells. Cell Stem Cell 10, 666-677 (2012
2. Ben-David, U. & Benvenisty, N. The tumorigenicity of human embryonic and induced pluripotent stem cells. Nat. Rev. Cancer 11, 268-277 (2011
3. Narsinh, K.H. et al. Single cell transcriptional profiling reveals heterogeneity of human induced pluripotent stem cells. J. Clin. Invest. 121, 1217-1221 (2011
4. Ghosh, Z. et al. Dissecting the oncogenic and tumorigenic potential of differentiated human induced pluripotent stem cells and human embryonic stem cells. Cancer Res. 71, 5030-5039 (2011
5. Lee, A.S. et al. Effects of cell number on teratoma formation by human embryonic stem cells. Cell Cycle 8, 2608-2612 (2009
6. Hentze, H. et al. Teratoma formation by human embryonic stem cells: Evaluation of essential parameters for future safety studies. Stem Cell Res. 2, 198-210 (2009
7. Ben-David, U., Nudel, N. & Benvenisty, N. Immunologic and chemical targeting of the tight-junction protein Claudin-6 eliminates tumorigenic human pluripotent stem cells. Nat. Commun. 4, 1992 (2013
8. Fong, C.Y., Peh, G.S., Gauthaman, K. & Bongso, A. Separation of SSEA-4 and TRA-1-60 labelled undifferentiated human embryonic stem cells from a heterogeneous cell population using magnetic-Activated cell sorting (MACS) and fluorescence-Activated cell sorting (FACS). Stem Cell Rev. 5, 72-80 (2009
9. Tang, C. et al. An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells. Nat. Biotechnol. 29, 829-834 (2011
10. Wang, Y.C. et al. Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis. Cell Res. 21, 1551-1563 (2011
11. Choo, A.B. et al. Selection against undifferentiated human embryonic stem cells by a cytotoxic antibody recognizing podocalyxin-like protein-1. Stem Cells 26, 1454-1463 (2008
12. Tan, H.L., Fong, W.J., Lee, E.H., Yap, M. & Choo, A. mAb 84, a cytotoxic antibody that kills undifferentiated human embryonic stem cells via oncosis. Stem Cells 27, 1792-1801 (2009
13. Blum, B., Bar-Nur, O., Golan-Lev, T. & Benvenisty, N. The anti-Apoptotic gene survivin contributes to teratoma formation by human embryonic stem cells. Nat. Biotechnol. 27, 281-287 (2009
14. Menendez, S. et al. Increased dosage of tumor suppressors limits the tumorigenicity of iPS cells without affecting their pluripotency. Aging Cell 11, 41-50 (2012
15. Schuldiner, M., Itskovitz-Eldor, J. & Benvenisty, N. Selective ablation of human embryonic stem cells expressing a 'suicide' gene. Stem Cells 21, 257-265 (2003
16. Rong, Z., Fu, X., Wang, M. & Xu, Y. A scalable approach to prevent teratoma formation of human embryonic stem cells. J. Biol. Chem. 287, 32338-32345 (2012
17. Eiges, R. et al. Establishment of human embryonic stem cell-Transfected clones carrying a marker for undifferentiated cells. Curr. Biol. 11, 514-518 (2001
18. Chung, S. et al. Genetic selection of sox1GFP-expressing neural precursors removes residual tumorigenic pluripotent stem cells and attenuates tumor formation after transplantation. J. Neurochem. 97, 1467-1480 (2006
19. Huber, I. et al. Identification and selection of cardiomyocytes during human embryonic stem cell differentiation. FASEB J. 21, 2551-2563 (2007
20. Ben-David, U. et al. Selective elimination of human pluripotent stem cells by an oleate synthesis inhibitor discovered in a high-Throughput screen. Cell Stem Cell 12 (2013
21. Dabir, D.V. et al. A small molecule inhibitor of redox-regulated protein translocation into mitochondria. Dev. Cell 25, 81-92 (2013
22. Tohyama, S. et al. Distinct metabolic flow enables large-scale purification of mouse and human pluripotent stem cell-derived cardiomyocytes. Cell Stem Cell 12, 127-137 (2013
23. Lee, M.O. et al. Inhibition of pluripotent stem cell-derived teratoma formation by small molecules. Proc. Natl. Acad. Sci. USA 110, E3281-E3290 (2013
24. Wang, L. et al. Claudin 6: A novel surface marker for characterizing mouse pluripotent stem cells. Cell Res. 22, 1082-1085 (2012
25. Dubois, N.C. et al. SIRPA is a specific cell-surface marker for isolating cardiomyocytes derived from human pluripotent stem cells. Nat. Biotechnol. 29, 1011-1018 (2011
26. Hattori, F. et al. Nongenetic method for purifying stem cell-derived cardiomyocytes. Nat. Methods 7, 61-66 (2010
27. Terstegge, S. et al. Laser-Assisted photoablation of human pluripotent stem cells from differentiating cultures. Stem Cell Rev. 6, 260-269 (2010
28. Schriebl, K. et al. Selective removal of undifferentiated human embryonic stem cells using magnetic activated cell sorting followed by a cytotoxic antibody. Tissue Eng. Part A 18, 899-909 (2012
29. Bieberich, E., Silva, J., Wang, G., Krishnamurthy, K. & Condie, B.G. Selective apoptosis of pluripotent mouse and human stem cells by novel ceramide analogues prevents teratoma formation and enriches for neural precursors in ES cell-derived neural transplants. J. Cell Biol. 167, 723-734 (2004
30. Tomizawa, M. et al. Survival of primary human hepatocytes and death of induced pluripotent stem cells in media lacking glucose and arginine. PLoS ONE 8, e71897 (2013
31. Cohen, D.E. & Melton, D. Turning straw into gold: Directing cell fate for regenerative medicine. Nat. Rev. Genet. 12, 243-252 (2011
32. Hannan, N.R., Segeritz, C.P., Touboul, T. & Vallier, L. Production of hepatocyte-like cells from human pluripotent stem cells. Nat. Protoc. 8, 430-437 (2013
33. Menendez, L. et al. Directed differentiation of human pluripotent cells to neural crest stem cells. Nat. Protoc. 8, 203-212 (2013
34. Shi, Y., Kirwan, P. & Livesey, F.J. Directed differentiation of human pluripotent stem cells to cerebral cortex neurons and neural networks. Nat. Protoc. 7, 1836-1846 (2012
35. Choi, K.D., Vodyanik, M. & Slukvin, II. Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells. Nat. Protoc. 6, 296-313 (2011
36. Ben-David, U., Mayshar, Y. & Benvenisty, N. Virtual karyotyping of pluripotent stem cells on the basis of their global gene expression profiles. Nat. Protoc. 8, 989-997 (2013
37. Ulitsky, I. et al. Expander: From expression microarrays to networks and functions. Nat. Protoc. 5, 303-322 (2010