Label-free imaging of lipid depositions in C. elegans using third-harmonic generation microscopy

PLoS ONE

Volumn 9, Issue 1, 2014, Pages

  Tserevelakis, George J ^a,b   Megalou, Evgenia V ^c   Filippidis, George ^a   Petanidou, Barbara ^a,b   Fotakis, Costas ^a,b   Tavernarakis, Nektarios ^b,c  

^a INSTITUTE OF ELECTRONIC STRUCTURE AND LASER   (Greece)

^b UNIVERSITY OF CRETE   (Greece)

^c INSTITUTE OF COMPUTER SCIENCE   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALS; CAENORHABDITIS ELEGANS; LIPID METABOLISM; LIPIDS; MICROSCOPY; MOLECULAR IMAGING; STAINING AND LABELING;

EID: 84894280973     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0084431     Document Type: Article

References (20)

1. Soukas AA, Kane EA, Carr CE, Melo JA, Ruvkun G (2009) Rictor/TORC2 regulates fat metabolism, feeding, growth, and life span in Caenorhabditis elegans. Genes Dev 23: 496-511.
2. Yen K, Le TT, Bansal A, Narasimhan SD, Cheng JX, et al. (2010) A comparative study of fat storage quantitation in nematode Caenorhabditis elegans using label and label-free methods. PLoS One 5: e12810.
3. Ranall MV, Gabrielli BG, Gonda TJ (2011) High-content imaging of neutral lipid droplets with 1,6-diphenylhexatriene. Biotechniques 51: 35-36, 38-42.
4. Min W, Freudiger CW, Lu S, Xie XS (2011) Coherent nonlinear optical imaging: beyond fluorescence microscopy. Annu Rev Phys Chem 62: 507-530.
5. Folick A, Min W, Wang MC (2011) Label-free imaging of lipid dynamics using Coherent Anti-stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) microscopy. Curr Opin Genet Dev 21: 585-590.
6. Barad Y, Eisenberg H, Horowitz M, Silberberg Y (1997) Nonlinear scanning laser microscopy by third harmonic generation. Applied Physics Letters 70: 922-924. (Pubitemid 127638748)
7. Debarre D, Beaurepaire E (2007) Quantitative characterization of biological liquids for third-harmonic generation microscopy. Biophys J 92: 603-612. (Pubitemid 46145793)
8. Debarre D, Supatto W, Beaurepaire E (2005) Structure sensitivity in third-harmonic generation microscopy. Opt Lett 30: 2134-2136. (Pubitemid 41251419)
9. Debarre D, Olivier N, Beaurepaire E (2007) Signal epidetection in third-harmonic generation microscopy of turbid media. Opt Express 15: 8913-8924. (Pubitemid 47112504)
10. Squier J, Muller M (2001) High resolution nonlinear microscopy: A review of sources and methods for achieving optimal imaging. Review of Scientific Instruments 72: 2855-2867. (Pubitemid 33606682)
11. Debarre D, Supatto W, Pena AM, Fabre A, Tordjmann T, et al. (2006) Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy. Nat Methods 3: 47-53. (Pubitemid 43135349)
12. Barros AG, Liu J, Lemieux GA, Mullaney BC, Ashrafi K (2012) Analyses of C. elegans fat metabolic pathways. Methods Cell Biol 107: 383-407.
13. Jones KT, Greer ER, Pearce D, Ashrafi K (2009) Rictor/TORC2 regulates Caenorhabditis elegans fat storage, body size, and development through sgk-1. PLoS Biol 7: e60.
14. McKay RM, McKay JP, Avery L, Graff JM (2003) C elegans: a model for exploring the genetics of fat storage. Dev Cell 4: 131-142. (Pubitemid 36105339)
15. Ashrafi K, Chang FY, Watts JL, Fraser AG, Kamath RS, et al. (2003) Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes. Nature 421: 268-272. (Pubitemid 36139130)
16. Wang MC, Min W, Freudiger CW, Ruvkun G, Xie XS (2011) RNAi screening for fat regulatory genes with SRS microscopy. Nat Methods 8: 135-138.
17. O'Rourke EJ, Soukas AA, Carr CE, Ruvkun G (2009) C. elegans major fats are stored in vesicles distinct from lysosome-related organelles. Cell Metab 10: 430-435.
18. Watts JL (2009) Fat synthesis and adiposity regulation in Caenorhabditis elegans. Trends Endocrinol Metab 20: 58-65.
19. Brock TJ, Browse J, Watts JL (2006) Genetic regulation of unsaturated fatty acid composition in C. elegans. PLoS Genet 2: e108.
20. Hermann GJ, Schroeder LK, Hieb CA, Kershner AM, Rabbitts BM, et al. (2005) Genetic analysis of lysosomal trafficking in Caenorhabditis elegans. Mol Biol Cell 16: 3273-3288. (Pubitemid 40965552)